Chromosome aberration assays in allium

Grant, W. F.

doi:10.1016/0165-1110(82)90046-x

Cited by 333 publications

(68 citation statements)

References 52 publications

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“…The A. cepa assay was performed according to a modified version of Grantʼs protocol (Grant 1982), and 2 treatments were conducted. The positive control was performed with methyl methane sulfonate 4⊗10…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Water Samples from a River by Cytologic Analysis in Allium cepa

2012

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SummaryThe potential induction of the chromosomic aberration of water samples from Santa Maria da Vitória River was evaluated by the Allium cepa test-system. This river supplies approximately 30% of the Grande Vitória region with water. The main environmental impact on this river are from domestic effluents from the municipal districts and agrotoxic contaminations. Water samples were collected in 5 sites in the rainy season (March-high temperatures) and in the dry season (August-low temperatures) of 2006, and 2 treatments-acute and chronic-were performed. The chromosomic aberration index was significantly higher in 2 sites of this river and also in the rainy season compared with the dry season. This genotoxic effect may be related to agricultural influence and to domestic effluents that occur close to these 2 sites and to the presence of fertilizers, pesticides and iron oxide from the basin soils that are leached out by the rain and reach the river.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Water Samples from a River by Cytologic Analysis in Allium cepa

2012

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“…In the A. cepa The cytotoxicity level of environmental assay, inhibition of root growth has been shown to pollutants can be determined by a decreased rate indicate retardation of growth and cytotoxicty of mitotic index (MI); this is an acceptable measure (Odeigah, et al, 1997;Grant, 1982). Also, growth inhibition can be caused by reduction in mitotic lead to aneuploidy and cell death (Kwon et al, 2008).…”

Section: Root Length Growth and Inhibition Farinmade (2013) The Mitomentioning

confidence: 99%

“…incomplete replication of chromosomes by defective or Binucleate formation therefore may be a way of less active replication enzymes (Sinha, 1979). Sticking doubling up the haploid genome, to produce diploid of chromosomes probably occurs due to degradation or cells of androgenetic origin as seen in placental depolymerization of chromosome DNA (Grant, 1982) or mesenchymal dysplasia, a rare condition associated as a result of DNA condensation and stickiness of interwith intrauterine growth restriction, prematurity chromosome fibers (Schneiderman et al, 1971). and intrauterine death.…”

Section: Root Length Growth and Inhibition Farinmade (2013) The Mitomentioning

confidence: 99%

Evaluation of Genotoxic Effect of Photographic Effluent Using Allium cepa Assay

Obidi¹,

Njoku²,

Akinmolayan³

2017

Nig. J. Biotechnol

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“…5, No. 3; disturbances in cell division cycle, including eventual aneuploidy risks (Grant, 1982;Fiskesjö, 1985;Leme & Marin-Morales, 2008). Allium cepa is used to study basic mechanisms as well as the effects of environmental contaminants on cell division and chromosome.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Mutagen-Induced Genotoxicity by two Lesotho Medicinal Plants in Allium cepa L

Asita¹,

Heisi²,

Tjale³

2015

ENRR

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Methanolic extracts (mg mL -1 ) of Dicoma anomala (0.0625, 0.125, 0.25), Chenopodium album (0.078, 0.156, 0.313), used in traditional medicine in Lesotho, and Camellia sinensis (0.1094, 0.2085, 0.44) were assessed for cytotoxicity, genotoxicity and modulation of Cyclophosphamide (CP 1.25 mg mL -1 ) -and EMS (0.25 mg mL -1 )-induced genotoxicity using the Allium cepa assay following 24 hours treatment. Cytotoxicity was measured by the mitotic index (MI). Genotoxicity (GT) was expressed as the number of aberrant mitotic cells per 100 mitotic cells. The MI and GT of test groups (triplicates), were compared with the negative (water) control group using t-test. Modulatory effect (ME) was calculated as, ME(%) = (1-(A-B)/(A-C) × 100) -1. A positive ME indicated an increase (synergism or potentiation) while a negative ME indicated a reduction (anti-genotoxicity) of mutagen-induced genotoxicity. The concentrations of D. anomala extract were cytotoxic and genotoxic. Mixtures of CP or EMS with the lower concentrations of D. anomala extract were more genotoxic than CP (250.50% increase) or EMS (149.74 and 157.37 % increase) or extracts alone. Only 0.313 mg mL -1 of C. album extract was cytotoxic but none of the three concentrations was genotoxic. Mixtures of CP with extracts of C.album were cytotoxic. CP-induced genotoxicity was reduced (55.18, 68.36, 57.40 %) and EMS-induced genotoxicity was also reduced by low concentrations (50.72, 61.13 %) of C.album extract. However, 0.313 mg mL -1 C. album extract increased (236.75%) EMS-induced genotoxicity. C. sinensis extracts and their mixtures with CP were not cytotoxic or genotoxic. CP-induced genotoxicity was reduced (63.61, 66.62, 78.64 %) but EMS-induced genotoxicity was increased (124.97, 4.48, 110.52 %) by C. sinensis extract.

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Chromosome aberration assays in allium

Cited by 333 publications

References 52 publications

Evaluation of Water Samples from a River by Cytologic Analysis in <i>Allium</i> cepa

Evaluation of Water Samples from a River by Cytologic Analysis in <i>Allium</i> cepa

Evaluation of Genotoxic Effect of Photographic Effluent Using<i> Allium cepa</i> Assay

Modulation of Mutagen-Induced Genotoxicity by two Lesotho Medicinal Plants in Allium cepa L

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