Chromosome 1 open reading frame 10 (C1orf10) gene is frequently down-regulated and inhibits cell proliferation in oral squamous cell carcinoma

Imai, Fabiana Lica; Uzawa, Katsuhiro; Nimura, Yuji; Moriya, Tsuneo; Imai, Massao A.; Shiiba, Masashi; Bukawa, Hiroki; Yokoe, Hidetaka; Tanzawa, Hideki

doi:10.1016/j.biocel.2005.02.005

Cited by 29 publications

(24 citation statements)

References 25 publications

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“…The percentage of CRNN-30 in G0/G1 phases was significantly increased ( P <0.01), whereas the percentage in S-phase was significantly decreased ( P <0.05), compared with that in control cells, suggesting that CRNN was able to arrest cell cycle at G1/S phase (Figure 3D). Similar results were also observed in CRNN-180 cells (Figure 3D), which is consistent with the previous report [17]. To further reveal the potential molecular mechanism of CRNN in cell cycle arrest, the effects of CRNN on key cell cycle regulators P53, P21 WAF1/CIP1 and Rb were tested.…”

Section: Resultssupporting

confidence: 91%

Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma

Chen

Dai

et al. 2013

PLoS ONE

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By using cDNA microarray analysis, we identified cornulin (CRNN) gene was frequently downregulated in esophageal squamous cell carcinoma (ESCC). In the present study, we investigated the role of CRNN in ESCC development. The results showed that CRNN was frequently downregulated in primary ESCCs in both mRNA level (26/56, 46.4%) and protein level (137/249, 55%), which was significantly associated with lymph node metastases (P=0.027), advanced clinical stage (P=0.039), and overall survival rate (P<0.001). Multivariate analysis indicated that the CRNN downregulation was an independent prognostic factor for ESCC. Functional studies with both in vitro and in vivo assays demonstrated that CRNN had strong tumor suppressive ability in ESCC cells. The tumor-suppressive mechanism of CRNN was associated with its role in cell cycle arrest at G1/S checkpoint by upregulating expressions of P21WAF1/CIP1 and Rb. Silencing CRNN expression by RNA interference could effectively inhibit its tumor suppressive effect. In conclusion, our findings demonstrate that CRNN is a tumor suppressor gene that plays a critical tumor suppressive role in ESCC.

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Section: Resultssupporting

confidence: 91%

Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma

Chen

Dai

et al. 2013

PLoS ONE

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“…Although it is known that the expression of these proteins, like many others, changes during the carcinogenic process (33,(41)(42)(43)(44)(45)(46)(47), no studies have been published that specifically elaborate on the prognostic value of these proteins to predict local relapses when analyzed on the resection margins of surgically treated HNSCC patients. Cornulin and small proline-rich protein 3 belong to the proteins forming the cornified envelope, an important protective barrier of the mucosa and skin.…”

Section: Discussionmentioning

confidence: 99%

Differential Proteomics Identifies Protein Biomarkers That Predict Local Relapse of Head and Neck Squamous Cell Carcinomas

Schaaij-Visser

Graveland

Gauci

et al. 2009

Clinical Cancer Research

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Purpose: The 5-year survival rates of head and neck squamous cell carcinomas (HNSCC) remain disappointing. HNSCCs develop in precursor fields of genetically altered cells that are often not completely resected when the tumor is excised, causing local relapse. These precursor fields are mostly recognized as dysplasia, but histologic grading cannot reliably predict malignant transformation. Our aim was to discover and validate protein biomarkers that can detect precursor fields and predict local relapse in HNSCC using immunostaining of surgical margins. Experimental Design: We compared paired and genetically characterized normal, precursor, and tumor tissues of eight patients by proteome analysis to identify differentially expressed proteins. The prognostic value of candidate protein biomarkers was evaluated by immunohistochemical analysis of 222 surgical margins of 46 HNSCC patients who developed local relapse or remained disease free. Significant associations were determined by Kaplan-Meier survival analysis and Cox-proportional hazards models. Results: Forty proteins showed significant differential expression (false discovery ratecorrected P < 0.05). Most discriminative markers suited for immunostaining were keratin 4 and cornulin. Low expression in the surgical margins of keratin 4 (hazard ratio, 3.8; P = 0.002), cornulin (hazard ratio, 2.7; P = 0.025), and their combination (hazard ratio, 8.8; P = 0.0005) showed a highly significant association with the development of local relapse. Dysplasia grading had no prognostic relevance. Conclusions: Immunohistochemical assessment of keratin 4 and cornulin expression in surgical margins of HNSCC patients outperforms histopathologic grading in predicting the risk for local relapse. These markers can be used to initiate more frequent and lifelong surveillance of patients at high risk of local relapse, and enable selection for adjuvant treatment or tertiary prevention trials. (Clin Cancer Res 2009;15(24):7666-75) Head and neck squamous cell carcinoma (HNSCC) develops in the mucosal linings of the upper aerodigestive tract and is the sixth most common cancer worldwide (1). The 5-year survival rates of HNSCC are approximately 60% (1) and have only moderately improved the last decades (2) mainly because 20% to 40% of all patients develop a local relapse in the same or adjacent anatomic region even when the surgical margins are histologically tumor free (3, 4). Clinically these relapses at the primary and adjacent anatomic sites are assigned as local recurrences when they develop within three years and at <2 cm distance of the primary tumor. Relapses not fulfilling these criteria are clinically classified as second primary tumors. Despite the difference in clinical assignment, many local relapses have in fact the same pathobiological origin (3-8).It has been well established that HNSCC is the result of a multistep process characterized by the accumulation of genetic and epigenetic alterations (9). Genetic analysis of surgical margins has shown that HNSCC frequent...

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“…Ex vivo organ culture in conjunction with specific stresses, including ethanol and heat shock, identified a novel class of stress proteins in normal squamous epithelium; these include SEP70, SEP53, and glutamine-glutamyl transferase (Hopwood et al 1997;Yagui-Beltran et al 2001). The SEP53 gene is located on chromosome 1q21 within a group of proteins named the epidermal differentiation complex fused-gene complex that is silenced as part of a general mechanism that suppresses genes from this locus in cancer cells Imai et al 2005). Molecular characterisation of SEP53 has demonstrated that its role as a stressresponsive protein is linked to its activity as an anti-apoptotic factor; death induced by exposure of cells to normally lethal levels of deoxycholic acid can be attenuated by SEP53 (Darragh et al 2006).…”

Section: Introductionmentioning

confidence: 99%

An animal model to evaluate the function and regulation of the adaptively evolving stress protein SEP53 in oesophageal bile damage responses

Nelson

Anderson

Archibald

et al. 2008

Cell Stress and Chaperones

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Squamous epithelium in mammals has evolved an atypical stress response involving down-regulation of the classic HSP70 protein and induction of sets of proteins including one named SEP53. This atypical stress response might be due to the unusual environmental pressures placed on squamous tissue. In fact, SEP53 plays a role as an anti-apoptotic factor in response to DNA damage induced by deoxycholic acid stresses implicated in oesophageal reflux disease. SEP53 also has a genetic signature characteristic of an adaptively and rapidly evolving gene, and this observation has been used to imply a role for SEP53 in immunity. Physiological models of squamous tissue are required to further define the regulation and function of SEP53. We examined whether porcine squamous epithelium would be a good model to study SEP53, since this animal suffers from a bile-reflux disease in squamous oesophageal tissue. We have (1) cloned and sequenced the porcine SEP53 locus from porcine bacterial artificial chromosome genomic DNA, (2) confirmed the strikingly divergent nature of the C-terminal portion of the SEP53 gene amongst mammals, (3) discovered that a function of the conserved N-terminal domain of the gene is to maintain cytoplasmic localisation, and (4) examined SEP53 expression in normal and diseased porcine pars oesophagea. SEP53 expression in porcine tissue was relatively confined to gastric squamous epithelium, consistent with its expression in normal human squamous epithelium. Immunohistochemical staining for SEP53 protein in normal and damaged pars oesophagea demonstrated significant stabilisation of SEP53 protein in the injured tissue. These results suggest that porcine squamous epithelium would be a robust physiological model to examine the evolution and function of the SEP53 stress pathway in modulating stress-induced responses in squamous tissue.

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Chromosome 1 open reading frame 10 (C1orf10) gene is frequently down-regulated and inhibits cell proliferation in oral squamous cell carcinoma

Cited by 29 publications

References 25 publications

Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma

Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma

Differential Proteomics Identifies Protein Biomarkers That Predict Local Relapse of Head and Neck Squamous Cell Carcinomas

An animal model to evaluate the function and regulation of the adaptively evolving stress protein SEP53 in oesophageal bile damage responses

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