Chromosomal location and distribution of As51 satellite DNA in five species of the genus Astyanax (Teleostei, Characidae, Incertae sedis)

Kantek, Daniel Luis Zanella; Vicari, Marcelo Ricardo; Peres, Wellington Adriano Moreira; Cestari, Marta Margarete; Artoni, Roberto Ferreira; Bertollo, Luiz Antônio Carlos; Moreira‐Filho, Orlando

doi:10.1111/j.1095-8649.2009.02333.x

Cited by 29 publications

(31 citation statements)

References 39 publications

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“…Satellite DNAs mobility, amplification, and potential to accumulate mutations play an important role in karyotype evolution, especially when these sequences are species-specific and/or chromosome-specific [Ugarkovic and Plohl, 2002] serving as potential tools for evolutionary and phylogenetic studies [Pons et al, 2002;Pons and Gillespie, 2003;Kantek et al, 2009;Vicari et al, 2010]. FISH with Cot-1 DNA probes showed that these repetitive fractions of the genome present a preferential centromeric location on the chromosomes of both species.…”

Section: Discussionmentioning

confidence: 99%

Comparative Cytogenetics of Giant Trahiras Hoplias aimara and H. intermedius (Characiformes, Erythrinidae): Chromosomal Characteristics of Minor and Major Ribosomal DNA and Cross-Species Repetitive Centromeric Sequences Mapping Differ among Morphologically Identical Karyotypes

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et al. 2010

Cytogenet Genome Res

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Karyotype and cytogenetic characteristics of 2 species of giant trahiras, Hopliasintermedius, São Francisco river basin, and Hopliasaimara, Arinos river (Amazon basin), were examined by conventional (C-banding, Ag-NOR, DAPI/CMA₃ double-staining) and fluorescent in situ hybridization (FISH) with 5S, 18S rDNA probes and cross-species Cot-1 DNA probing. Both species invariably had diploid chromosome number 2n = 50 and identical karyotypes composed of 10 pairs of metacentric and 15 pairs of submetacentric chromosomes. On the other hand, staining with base-specific fluorochromes (CMA₃, DAPI) and FISH mapping of repetitive DNA sequences showed extensive interspecific differences: while the genome of H. aimara had one submetacentric pair bearing CMA₃-positive (DAPI-negative) sites, that of H. intermedius had 4 such pairs; while FISH with a 5S rDNA probe showed one (likely homologous) signal-bearing pair, that with 18S rDNA displayed one signal-bearing pair in H. intermedius and 2 such pairs in H. aimara. Cross-species FISH probing with Cot-1 DNA prepared from total DNA of both species showed no signals of Cot-1 DNA from H. aimara on chromosomes of H. intermedius but reciprocally (Cot-1 DNA from H. intermedius on chromosomes of H. aimara) displayed signals on at least 4 chromosome pairs. Present findings indicate (i) different composition of repetitive sequences around centromeres, (ii) different NOR phenotypes and (iii) distinct taxonomic status of both giant trahira species.

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Section: Discussionmentioning

confidence: 99%

Comparative Cytogenetics of Giant Trahiras Hoplias aimara and H. intermedius (Characiformes, Erythrinidae): Chromosomal Characteristics of Minor and Major Ribosomal DNA and Cross-Species Repetitive Centromeric Sequences Mapping Differ among Morphologically Identical Karyotypes

Blanco

Lui

Vicari

et al. 2010

Cytogenet Genome Res

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“…However, reassociation kinetics based on C o t-1 DNA and the microdissection of chromosomes submitted to C-banding and the subsequent amplification of heterochromatic sequences using degenerate oligonucleotide-primed polymerase chain reaction (PCR) have been used successfully. [9][10][11][12][13][14] The Parodontidae is a Neotropical fish group that is divided into three genera: Parodon, Apareiodon, and Saccodon. 15 Cytogenetic studies reported a conserved diploid number of 54 chromosomes in Parodontidae with distinctive karyotype formulae and numerical/position variation of 18S and 5S rDNA sites.…”

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Construction and Characterization of a Repetitive DNA Library in Parodontidae (Actinopterygii: Characiformes): A Genomic and Evolutionary Approach to the Degeneration of the W Sex Chromosome

et al. 2014

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Repetitive DNA sequences, including tandem and dispersed repeats, comprise a large portion of eukaryotic genomes and are important for gene regulation, sex chromosome differentiation, and karyotype evolution. In Parodontidae, only the repetitive DNAs WAp and pPh2004 and rDNAs were previously studied using fluorescence in situ hybridization. This study aimed to build a library of repetitive DNA in Parodontidae. We isolated 40 clones using C o t-1; 17 of these clones exhibited similarity to repetitive DNA sequences, including satellites, minisatellites, microsatellites, and class I and class II transposable elements (TEs), from Danio rerio and other organisms. The physical mapping of the clones to chromosomes revealed the presence of a satellite DNA, a Helitron element, and degenerate short interspersed element (SINE), long interspersed element (LINE), and tc1-mariner elements on the sex chromosomes. Some clones exhibited dispersed signals; other sequences were not detected. The 5S rDNA was detected on an autosomal pair. These elements likely function in the molecular degeneration of the W chromosome in Parodontidae. Thus, the location of these elements on the chromosomes is important for understanding the function of these repetitive DNAs and for integrative studies with genome sequencing. The presented data demonstrate that an intensive invasion of TEs occurred during W sex chromosome differentiation in the Parodontidae.

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“…1a). In Teleostei, chromosomespecific satellite DNA may be a useful cytogenetic marker (Saito et al 2007), and sometimes used to in resolving cytotaxonomic issues in species complexes such as that involving the Astyanax scabripinnis species group (Vicari et al 2008;Kantek et al 2009). Among belonids, the heterochromatin can be considered an important component in the karyotype diversification of the group.…”

Section: Discussionmentioning

confidence: 99%

Comparative cytogenetic analysis of marine needlefishes (Beloniformes) from southern Brazil

et al. 2014

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Cytogenetic studies have assisted in the taxonomic classification of organisms, especially those involving species with highly similar morphologic characteristics, or so-called cryptic species. Strongylura marina and Strongylura timucu collected from Paranaguá Bay, Paraná Coast in Southern Brazil are considered cryptic species, and the identification of interspecific variations based on the number and/or morphology of its chromosomes may serve as differentiating cytotaxonomic markers. Chromosomes of the two species were subjected to different banding and staining methods (C-, Ag-, and DAPI-CMA 3 ), as well as chromosomal mapping of major rDNA (45S), revealed with an 18S probe by fluorescence in situ hybridization (FISH). The pattern of distribution of constitutive heterochromatin showed distinct features involving the pericentromeric and telomeric bands in both species. In S. marina, chromosome 1 represents the main species-specific marker, appearing almost entirely heterochromatic. In both species, the 45S rDNA is located at terminal region of the short arm of the chromosome 6, as detected by silver nitrate staining and FISH. Despite the apparent conserved diploid number of 48 chromosomes, data on the karyotype microstructure characterize the cytogenetic profile of the genus and may allow the establishment of cytotaxonomic and evolutionary inferences for these fishes.

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Chromosomal location and distribution of As51 satellite DNA in five species of the genus Astyanax (Teleostei, Characidae, Incertae sedis)

Cited by 29 publications

References 39 publications

Comparative Cytogenetics of Giant Trahiras <i>Hoplias aimara </i>and <i>H. intermedius </i>(Characiformes, Erythrinidae): Chromosomal Characteristics of Minor and Major Ribosomal DNA and Cross-Species Repetitive Centromeric Sequences Mapping Differ among Morphologically Identical Karyotypes

Comparative Cytogenetics of Giant Trahiras <i>Hoplias aimara </i>and <i>H. intermedius </i>(Characiformes, Erythrinidae): Chromosomal Characteristics of Minor and Major Ribosomal DNA and Cross-Species Repetitive Centromeric Sequences Mapping Differ among Morphologically Identical Karyotypes

Construction and Characterization of a Repetitive DNA Library in Parodontidae (Actinopterygii: Characiformes): A Genomic and Evolutionary Approach to the Degeneration of the W Sex Chromosome

Comparative cytogenetic analysis of marine needlefishes (Beloniformes) from southern Brazil

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