Chromosomal aberrations induced by chemotherapy and radiotherapy in lymphocytes from patients with breast carcinoma

Crevoisier, R. de; Lartigau, É.; Morsli, Karim; Dossou, Joseph; Chavaudra, N.; Sanfilippo, Nicolas; Bourhis, Jean; Eschwège, F.; Parmentier, C

doi:10.1016/s0360-3016(01)02749-3

Cited by 17 publications

(6 citation statements)

References 40 publications

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“…Our findings, that the CAs increase in the lymphocytes of HNSCC patients following RT, complements the above report. Further, Legal et al also observed significant increase of CAs in breast cancer patients after external irradiation [24]. In addition to this, the increased CAs in the lymphocytes of cancer patient's was observed by Martin et al following RT also supports our findings.…”

Section: Discussionsupporting

confidence: 91%

Cytogenetic Analysis on the Yields of Chromosomal Aberrations Induced by the Scattered Doses of γ-Radiation

Kadam¹,

Shyama²

2016

J Nucl Med Radiat Ther

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The present study is undertaken to know the radiation dose-response of chromosomes in the non-target cells, viz. peripheral blood lymphocytes of cancer patients in a context of partial body irradiation. The genotoxic effect of γ-radiation was studied in Head and Neck Squamous Cell Carcinoma (HNSCC) patients exposed to various cumulative doses of 60 Co gamma rays during radiotherapy (RT). These patients (P 1 to P 10 ) were irradiated for a period of six weeks with a daily fraction of 2 Gy, consecutively for 5 days in every week. The clastogenic effects of radiation in these patients were analysed on every weekend employing chromosomal aberration (CA) assay. Radiosensitivity of these patients were analysed by employing linear regression analysis of the CAs induced by irradiation. Genetic damage observed in all patients on a weekly basis were recorded and analysed at the individual level in comparison with their own pre-therapy baseline data, employing student's t-test. Dicentrics, centric rings and chromatid breaks were observed as the major kinds of CAs. The total CAs observed were analyzed using two way ANOVA, showed significant (P<0.001) intra and inter-individual variations of the genotoxic effects. Further, a dose dependent increase of cytogenetic damage was observed in the non-target cells viz. lymphocytes. Further, cytogenetic studies in peripheral lymphocytes following gamma radiotherapy of tumors may help to understand the optimum/precise dose of radiation to be employed for RT, may also be useful to predict dosimetry and the possible secondary tumors in irradiated HNSCC patients.

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Section: Discussionsupporting

confidence: 91%

Cytogenetic Analysis on the Yields of Chromosomal Aberrations Induced by the Scattered Doses of γ-Radiation

Kadam¹,

Shyama²

2016

J Nucl Med Radiat Ther

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“…Thus, the common denominator of radiation damage to many tissues is related to vascular effects. We therefore may point to the nature of the irradiated volume containing large blood vessels as the major determinant of the observed biologic effects (Legal et al 2002, Sindelar & Kinsella 2003. Hematopoietic tissues exposed to ionizing radiation are known to exhibit macrophage activation, enhanced respiratory burst activities and neutrophil infiltration (Lorimore et al 2001), and a heritable, genomewide process of instability in the descendants of hematopoietic stem cells in vivo (Mcilrath et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Peripheral leukocyte response to oncological radiotherapy: Expression of heat shock proteins

Guisasola¹,

Marcos

Şimon³

et al. 2006

International Journal of Radiation Biology

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“…Several studies have been performed on inducing CAs in human lymphocytes by radiation. Legal et al (2002) (40) reported increased CAs frequency after RT and chemotherapy in lymphocytes of patients with breast carcinoma. A similar study compared CAs in human sperm and lymphocytes before and after in vivo radiation treatment of 13 cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the types and frequency of unstable chromosomal aberrations induced in lymphocytes of breast cancer patients before and after radiotherapy

Mozdarani

Parvaneh²,

Mozdarani³

et al. 2022

Int J Radiat Res

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Background: There is not yet an appropriate biomarker to predict or follow radiosensitivity of Breast cancer (BC) patients during or after radiotherapy. The aim of this study was to monitor chromosomal aberrations (CA) induced before and during radiotherapy in peripheral blood lymphocytes of BC patients. Materials and Methods: Age-matched twenty normal healthy individuals and 20 invasive ductal BC patients were enrolled in this study. A blood sample was obtained from normal healthy women and BC patients before and after the first, two and four weeks after radiotherapy. Lymphocyte microculture was initiated in 4.5ml complete RPMI-1640 medium. Cells were harvested 50 hours after culture initiation. Cells were harvested based on standard protocols. Hundreds of well-spread mitoses were scored under a light microscope with a magnification of x1000 for various types of CA. Data were statistically analyzed and p<0.05 was considered a significant difference. Results: Results indicated a higher frequency of CA in lymphocytes of un-irradiated BC patients compared to healthy normal individuals, although not statistically significant (p>0.05). High frequencies of CA were observed in lymphocytes of BC patients after radiotherapy, significantly different from the un-irradiated group (p<0.01). The increase in the frequency of CA was increased with increasing radiation dose. Conclusion: Genome instability may contribute to high background and radiationinduced CA in lymphocytes of BC patients. However, there is also the possibility of a radio-adaptation of cells during the course of radiotherapy. Results imply that dicentric chromosomes might be valuable cytogenetic bioindicators to monitor the response of BC patients to radiotherapy.

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Chromosomal aberrations induced by chemotherapy and radiotherapy in lymphocytes from patients with breast carcinoma

Cited by 17 publications

References 40 publications

Cytogenetic Analysis on the Yields of Chromosomal Aberrations Induced by the Scattered Doses of γ-Radiation

Cytogenetic Analysis on the Yields of Chromosomal Aberrations Induced by the Scattered Doses of γ-Radiation

Peripheral leukocyte response to oncological radiotherapy: Expression of heat shock proteins

Evaluation of the types and frequency of unstable chromosomal aberrations induced in lymphocytes of breast cancer patients before and after radiotherapy

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