Chromosomal aberrations in human lymphocytes exposed in vitro to enrofloxacin and ciprofloxacin

Gorla, Nora; Ovando, Hugo García; Larripa, Irene

doi:10.1016/s0378-4274(98)00230-6

Cited by 33 publications

(18 citation statements)

References 14 publications

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“…These compounds target the A subunit of DNA gyrase and are effective inhibitors of this enzyme (Curry et al, 1996). The most active compounds with the broad antibacterial spectrum are the C-6 fluorinated quinolones and 1-8-naphthyridines that have a cyclic amino group at position 7 (Radl, 1990) as ENR and its principal metabolite CIP (Gorla et al, 1999). However, quinolones have exhibited varying degrees of cross-reactivity with mammalian topoisomerase II, which is a gyrase-like enzyme, and certainly with other replication enzymes (Bredberg et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, NA was reported to stimulate unscheduled DNA synthesis in rat splenic cells and thymic cells in culture, but not in primary rat hepatocyte. ENR and CIP have been described to induce chromosomal aberrations in cultures of human lymphocytes at concentrations varying between 5 and 50 mg/L for ENR and 5 and 25 mg/L for CIP (Gorla et al, 1999). A reduction in the mitotic index and fuzzy metaphases were observed at 50 mg/L of ciprofloxacin, indicating a cytotoxic effect of this compound.…”

Section: Discussionmentioning

confidence: 99%

“…For example, exposure of Chinese hamster ovary cells or Syrian hamster embryo cells to nalidixic acid (NA) and ciprofloxacin (CIP) induced cytotoxic effects and micronucleus induction (Gibson et al, 1998). In vitro studies with human lymphocytes exposed to enrofloxacin (ENR) and ciprofloxacin revealed an increase in the chromosomal aberrations, detected as chromatid and chromosome breaks and gaps (Gorla et al, 1999). FQs have been reported to cause neonatal alterations in articulation cartilages, bone growth and tendons, both in humans and animals (Patterson, 1991;Gough et al, 1992;Hildebrand et al, 1993;Forster et al, 1996;Simonin et al, 1999;Stahlmann, 2003;Lemus et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Khadra¹,

Pinelli²,

Lacroix³

et al. 2012

Ecotoxicology and Environmental Safety

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, et al.. Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test. Ecotoxicology and Environmental Safety, Elsevier, 2012, 76, pp.187-192. <10.1016/j.ecoenv.2011 a b s t r a c tThe genotoxicity of quinolone and fluroquinolones was assessed using the micronucleus (MN) test on Vicia faba roots by direct contact exposure to a solid matrix. Plants were exposed to quinolones (nalidixic acid) and fluoroquinolones (ciprofloxacin and enrofloxacin) alone or mixed with artificially contaminated soils. Four different concentrations of each of these antibiotics were tested (0.01, 0.1, 1 and 10 mg/Kg) for nalidixic acid and (0.005, 0.05, 0.5 and 5 mg/Kg) for ciprofloxacin and enrofloxacin. These antibiotics were also used in mixture. Exposure of Vicia faba plants to each antibiotic at the highest two concentrations showed significant MN induction. The lowest two concentrations had no significant genotoxic effect. The mixture of the three compounds induced a significant MN induction whatever the mixture tested, from 0.02 to 20 mg/Kg. The results indicated that a similar genotoxic effect was obtained with the mixture at 0.2 mg/Kg in comparison with each molecule alone at 5-10 mg/Kg. Data revealed a clear synergism of these molecules on Vicia faba genotoxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Khadra¹,

Pinelli²,

Lacroix³

et al. 2012

Ecotoxicology and Environmental Safety

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show abstract

“…Fluoroquinolone antibiotic residues of enrofloxacin (ENR) and ciprofloxacin (CIP) in muscle and tissue have proved to be potential risk for health [1]. Several methods had been proposed for determination of ENR and CIP in biological samples [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous extraction of enrofloxacin and ciprofloxacin from chicken tissue by molecularly imprinted matrix solid-phase dispersion

Qiao

Sun

2010

Journal of Pharmaceutical and Biomedical Analysis

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“…In several animal species, ENR is de-ethylated to its primary metabolite, ciprofloxacin (CPX), and both ENR and CPX are found in the edible products of animals receiving ENR [4,5]. In recent years, the use of these compounds in veterinary application has been subject to regulation, because their residues may persist in edible animal products and may result in the development of drug-resistant bacterial strains or allergies [6]. Many countries have introduced Maximum Residue Limits of ENR and CPX in food products, such as 100 lg/kg established by the European Union for the sum of enrofloxacin and its metabolite ciprofloxacin in milk [7,8].…”

Section: Introductionmentioning

confidence: 99%

Determination of enrofloxacin and ciprofloxacin in milk using molecularly imprinted solid‐phase extraction

Yan

Tian

Row

2008

J of Separation Science

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A molecularly imprinted solid-phase extraction procedure was developed for the simultaneous identification of enrofloxacin and its active metabolite, ciprofloxacin, in milk samples. Water-compatible molecularly imprinted polymers synthesized in a water-methanol system show a high degree of cross-reactivity for enrofloxacin and ciprofloxacin in aqueous environments. The imprinted particles were applied as selective sorbents in a solid-phase extraction process focusing upon complex milk matrices, which allowed the matrix compounds present in milk samples to be removed effectively. The extracts were sufficiently clean for further chromatographic analysis, and no interference originating from the biological matrix was observed. The mean recoveries of enrofloxacin and ciprofloxacin from milk sample were 82.6-93.5% and 81.2-94.8%, respectively, with the RSD less than 7.5%. This method is simple and sensitive, and is therefore an alternative tool to the existing HPLC methods for analyzing residual enrofloxacin and ciprofloxacin in biological samples.

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Chromosomal aberrations in human lymphocytes exposed in vitro to enrofloxacin and ciprofloxacin

Cited by 33 publications

References 14 publications

Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Simultaneous extraction of enrofloxacin and ciprofloxacin from chicken tissue by molecularly imprinted matrix solid-phase dispersion

Determination of enrofloxacin and ciprofloxacin in milk using molecularly imprinted solid‐phase extraction

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