“…The log 2 ratio Cy5/Cy3 value of lost chromosomes is negative (−1), whereas the value of log 2 ratio Cy5/Cy3 of chromosomes that are neither gained nor deleted (no CNV) is 0. B-D, genomic profiles of cases 1 B, 2 C, and 3 D, showing gains of chromosomes 4,7,8,9,11,12,14,15,16,18,19,20, and 22 B; gains of chromosomes 4,7,8,11,12,14,15,16,18,19,20,22, and X, C; gains of chromosomes 4, 5, 7, 8q, 11, 12, 14, 15, 16, 18p, 19, 20p, and 22, D. The unexpected presence of gains instead of classical losses of chromosomes 1, 2, 6, 10, 13, 17, and 21 raised the hypothesis of an inappropriate threshold of normalization in the context of a polyploid genome: the positive log 2 ratio (+1) might in fact be "0" while log 2 ratio "0" might be negative (−1). The modification of the threshold (recentring) provided a result in accordance to expected losses of chromosomes [Color figure can be viewed at wileyonlinelibrary.com] T A B L E 1 Clinical and genetic characteristics of our series of ChRCC 1/173 365 73/F CChRCC pT3aNxMx RN +4, +7, +8, +9, +11, +12, +14, +15, +16, +18, +19, +20, +22 (Ag)/−1,-2,-3,-5,-6, −10, −13, − >0.2 and genomic loss by a log2 ratio <0.2.…”