Chromophobe Cell Renal Carcinoma: DNA Flow Cytometry and Proliferation Status in 9 Cases

Abstract

Nine cases of chromophobe cell renal carcinoma are reported, 2 in pregnant women. One neoplasm progressed and the patient died of disease, and this tumor presented the highest mitotic index and focal positivity to Ki-67 antibody. DNA flow-cytometric study discovered 89% of aneuploidy, with 3 tetraploid cases. We conclude that chromophobe cell renal carcinoma is a relatively indolent but clearly malignant neoplasm. We suggest that the mitotic index could be the best method to predict malignant behavior. The high incidence of aneuploid cell lines may be due to structural anomalies; it is not indicative of malignant behavior.

Detection of tetraploidization in chromophobe renal cell carcinoma: Insights and pitfalls

“…Tetraploidization in ChRCC has been also described in some studies using flow cytometry. [11][12][13][14][15] In the present study, we associated with a poor prognosis in many types of solid cancer, [28][29][30] including renal cancer. 31 On the one hand, according to Casuscelli et al, 16 it may directly be related, in association with TP53 and PTEN mutations, to metastatic evolution in ChRCC, while according to Brunelli et al, 32 it might be related to the presence of a sarcomatoid component rather than metastatic evolution.…”

“…The log 2 ratio Cy5/Cy3 value of lost chromosomes is negative (−1), whereas the value of log 2 ratio Cy5/Cy3 of chromosomes that are neither gained nor deleted (no CNV) is 0. B-D, genomic profiles of cases 1 B, 2 C, and 3 D, showing gains of chromosomes 4,7,8,9,11,12,14,15,16,18,19,20, and 22 B; gains of chromosomes 4,7,8,11,12,14,15,16,18,19,20,22, and X, C; gains of chromosomes 4, 5, 7, 8q, 11, 12, 14, 15, 16, 18p, 19, 20p, and 22, D. The unexpected presence of gains instead of classical losses of chromosomes 1, 2, 6, 10, 13, 17, and 21 raised the hypothesis of an inappropriate threshold of normalization in the context of a polyploid genome: the positive log 2 ratio (+1) might in fact be "0" while log 2 ratio "0" might be negative (−1). The modification of the threshold (recentring) provided a result in accordance to expected losses of chromosomes [Color figure can be viewed at wileyonlinelibrary.com] T A B L E 1 Clinical and genetic characteristics of our series of ChRCC 1/173 365 73/F CChRCC pT3aNxMx RN +4, +7, +8, +9, +11, +12, +14, +15, +16, +18, +19, +20, +22 (Ag)/−1,-2,-3,-5,-6, −10, −13, − >0.2 and genomic loss by a log2 ratio <0.2.…”

“…Notably, information on polyploidized ChRCC is scarce. [7][8][9][10][11][12][13][14][15][16] Here, we report a comprehensive clinical, histological and molecular genetic study of five novel cases of tetraploidized ChRCC. We present the methodological pitfalls of detection of this tetraploidy and we discuss the potential mechanisms and possible diagnostic and prognostic consequences of this underestimated phenomenon.…”

“…and inFigures 1, 4, 5 and Supplementary respectively. Agilent-aCGH showed gains of chromosomes4,7,8,11,12,14,15,16,18,19,20,22, and X (Figure 1C), whereas Affymetrix-aCGH showed losses of chromosomes 1, 2, 3, 5, 6, 9, 10, 13, 17, and 21 (Figure 4C). The BAF and allele difference profiles indicated a monoallelic origin of chromosomes 1, 2, 3, 5, 6, 9, 10, 13, 17, and 21 (Figure 5C).…”

Spontanruptur einer Niere post partum aufgrund eines urothelialen Karzinoms

“…Walker u. Knight [36] berichteten über 35 Fälle zwischen 1890 und 1986. Seit 1986 wurden 18 weitere Fälle berichtet [2,8,9,15,18, 20±23, 26±27, 30±33]. Unter der Entität der, in der Schwangerschaft diagnostizierten Neoplasien der Niere haben Nierenzellkarzinome mit bis zu 50 % einen hohen Anteil [36].…”