Chromolaena laevigata (Asteraceae) as a source of endophytic non-aflatoxigenic Aspergillus flavus: chemical profile in different culture conditions and biological applications

Balbinot, Rodolfo Bento; Oliveira, Josiane A Monteiro de; Bernardi, Darlon I.; Polli, Andressa Domingos; Polônio, Julio Cesar; Cabral, Márcia Regina Pereira; Zanqueta, Érica Benassi; Endo, Eliana Harue; Meneguello, Jean Eduardo; Cardoso, Rosilene Fressatti; Azevedo, João Lúcio de; Ueda-Nakamura, Tânia; Carmo, Marta Regina Barrotto do; Sarragiotto, María Helena; Pamphile, João Alencar; Baldoqui, Débora Cristina

doi:10.1007/s42770-021-00502-6

Cited by 4 publications

(5 citation statements)

References 72 publications

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“…HPLC analysis showed absence of aflatoxins B1, B2, G1, and G2 in the culture filtrate of A. flavus YRB2, confirming that it is non-aflatoxigenic. This result is in agreement with that obtained by Balbinot et al [46] who isolated the nonaflatoxigenic, endophytic A. flavus CL7 from the wild shrub Chromolaena laevigata. Isolation of non-aflatoxigenic strains of A. flavus has been widely reported in the last years, where used as a promising strategy for biocontrol of aflatoxigenic strains, and prevention/ reduction of aflatoxin contamination under field conditions [47].…”

Section: Discussionsupporting

confidence: 93%

Aspergillus flavus YRB2 from Thymelaea hirsuta (L.) Endl., a non-aflatoxigenic endophyte with ability to overexpress defense-related genes against Fusarium root rot of maize

2022

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Fusarium root rot, caused by Fusarium solani (Mart.) Sacc., represents one of the most damaging diseases of maize affecting plant growth and yield. In this study, the antagonistic potential of a non-aflatoxigenic endophytic Aspergillus flavus YRB2, isolated from Thymelaea hirsuta (L.) Endl., was tested against F. solani in vitro. In addition, its biocontrol activity against Fusarium root rot of maize was evaluated under greenhouse conditions. Its impacts on plant molecular, pathological, physiological, and growth levels were also studied. Results obtained revealed a potent antagonistic behavior for A. flavus YRB2 against F. solani in vitro, recording 80% growth inhibition. Seventeen secondary metabolites were detected in the n-hexane extract of A. flavus YRB2 filtered culture broth using GC-MS analysis. Among them, various antifungal secondary metabolites were produced, namely palmitic acid, α-linolenic acid, stearic acid, 2, 4-di-tert-butylphenol, diisobutyl phthalate, and heneicosane. In contrast, HPLC analysis showed that no aflatoxins (B1, B2, G1, and G2) were detected. Under greenhouse conditions, colonization of maize plants with A. flavus YRB2 exhibited a potential biocontrol activity against Fusarium root rot, recording 73.4% reduction in the disease severity. Triggering of transcriptional expression level of the defense-related genes JERF3 (7.2-fold), CHI II (8-fold), and POD (9.1-fold) was reported, indicating the inducing effect on the plant immunity. In addition, an increment in the antioxidant enzymes POD and PPO, and the total phenolic content in maize roots was also observed in response to this treatment. Moreover, a growth-promoting effect was also observed for colonization of maize plants with A. flavus YRB2. Based on the obtained data, we can conclude that A. flavus YRB2 may represent a promising biocontrol and growth-promoting agent for maize plants against Fusarium root rot. Nevertheless, field evaluation is highly requested before the use recommendation.

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Section: Discussionsupporting

confidence: 93%

Aspergillus flavus YRB2 from Thymelaea hirsuta (L.) Endl., a non-aflatoxigenic endophyte with ability to overexpress defense-related genes against Fusarium root rot of maize

2022

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“…It has a straightforward biochemical structure with the nucleic acid surrounded by a protein coat, though some viruses have a lipoprotein membrane or envelope. This envelope consists of a lipid bilayer that generally contains exposed glycoproteins on the surface, including the main viral antigens [46] …”

Section: Viral Activitymentioning

confidence: 99%

“…In previous studies, we reported the potential of endophytes against several types of microorganisms, including protozoa [7] and bacteria, [8] and described the main compounds produced, such as linoleic acid, [7] mellein and β-orcinaldehyde, [44,45] kojic acid, α-cyclopiazonic acid, and 20,25-dihydroxyaflavinine. [46] Thus, the study of the endophyte-host relationship can establish alternative sources of substances of biotechnological interest. This review discusses the possible antiviral qualities of endophytic organisms.…”

Section: Endophytic Fungimentioning

confidence: 99%

“…This envelope consists of a lipid bilayer that generally contains exposed glycoproteins on the surface, including the main viral antigens. [46] The lipids present in enveloped viruses are sensitive to solvents, such as chemical disinfectants and ethers. When lipids are dissolved because of this sensitivity, enveloped viruses lose their ability to infect, as their infectious mechanisms are based on protein fusion on the surface of the viral envelope with host cell receptors.…”

Section: Viral Activitymentioning

confidence: 99%

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Endophytic Fungi as a Source of Antiviral Compounds – A Review

Lacerda

Polônio

Golias

2022

Chemistry & Biodiversity

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Endophytic fungi are a rich source of secondary metabolites. The interactions between endophytes and their hosts lead to the production of several bioactive substances grouped into different classes, each having a wide variety of effects against various pathogens. The metabolites obtained from these organisms include steroids, alkaloids, phenols, isocoumarins, xanthones, quinones, and terpenoids, among others. These substances are known to have antibiotic, antiparasitic, antifungal, and antiviral effects. This review summarizes secondary metabolites with antiviral effects produced by endophytic fungi and highlights the importance of research in developing novel antiviral substances. We demonstrate that endophytic fungi are a rich source of secondary metabolites that combat pathologies caused by viruses. Optimizing practical and biotechnological screening tools for the research of these metabolites will provide promising drugs to combat these infections.

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“…Após a obtenção do caldo fermentado, realiza-se a separação das células microbianas do caldo fermentado por centrifugação e/ou filtração e, do caldo fermentado resultante ocorre a extração dos metabólitos secundários empregando solventes orgânico adequados. O principal solvente empregado neste processo de extração é o Acetato de Etila (POLONIO et al 2016;SANTOS et al 2017;GOLIAS et al 2019;POLLI et al 2020;BALBINOT et al 2021;OLIVEIRA et al 2020;SILVA et al 2021). O solvente resultante da extração contendo os metabólitos secundários passa pelo processo de evaporação para a concentração dos metabólitos.…”

Section: Introductionunclassified

Técnicas De Identificação Química De Metabólitos Produzidos Por Microrganismos Endofíticos

Oliveira¹,

Misturini²

2022

arqmudi

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Os microrganismos endofíticos colonizam o interior dos tecidos das plantas sem causar danos ao seu hospedeiro. A interação endófito-planta pode originar vias de síntese de metabólitos de interesse biotecnológico, contudo, para sua utilização é necessário que esses compostos sejam identificados quimicamente. Dentre as técnicas instrumentais mais utilizadas estão a cromatografia líquida de alta eficiência (CLAE), cromatografia em camada delgada (CCD), cromatografia a gás capilar (CG), cromatografia a gás capilar acoplada a espectrometria de massas (CG/MS), cromatografia de fluído supercrítico (CFS) e eletroforese capilar (EC). Desta forma, realizamos uma revisão da literatura abordando estas técnicas de identificação de metabólitos secundários de interesse biotecnológico produzidos por microrganismos endofíticos. A pesquisa revelou que estas técnicas instrumentais permitiram a identificação de coclioquinonas, isocloquinonas, dicetopiperazinas, ácido tartárico, ácido cítrico, butirilneosolaniol e outros compostos, produzidos por microrganismos endofíticos. Estas técnicas instrumentais para identificação e quantificação de metabólitos são amplamente utilizadas e aperfeiçoadas. É possível destacar que para cada característica de amostra e sua finalidade, alguns dos métodos se tornam mais eficientes e precisos. Esses métodos para análise de metabólitos devem continuar evoluindo a fim de descobrir novas moléculas e também gerar uma maior robustez para a identificação e quantificação destes compostos de origem microbiana, contribuindo para o avanço dos produtos naturais.

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Chromolaena laevigata (Asteraceae) as a source of endophytic non-aflatoxigenic Aspergillus flavus: chemical profile in different culture conditions and biological applications

Cited by 4 publications

References 72 publications

Aspergillus flavus YRB2 from Thymelaea hirsuta (L.) Endl., a non-aflatoxigenic endophyte with ability to overexpress defense-related genes against Fusarium root rot of maize

Aspergillus flavus YRB2 from Thymelaea hirsuta (L.) Endl., a non-aflatoxigenic endophyte with ability to overexpress defense-related genes against Fusarium root rot of maize

Endophytic Fungi as a Source of Antiviral Compounds – A Review

Técnicas De Identificação Química De Metabólitos Produzidos Por Microrganismos Endofíticos

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