Chromobacterium violaceum and Pseudomonas aeruginosa PAO1: Models for Evaluating Anti-Quorum Sensing Activity of Melaleuca alternifolia Essential Oil and Its Main Component Terpinen-4-ol

Noumi, Emira; Merghni, Abderrahmen; Alreshidi, Mousa; Haddad, Ons; Akmadar, Gültekin; Martino, Laura De; Mastouri, Maha; Ceylan, Özgür; Snoussi, Mejdi; Al‐Sieni, Abdulbasit I.; Feo, Vincenzo De

doi:10.3390/molecules23102672

Cited by 60 publications

(45 citation statements)

References 51 publications

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“…In earlier studies, inhibition zones of 10 mm and 13 mm of violacein production were reported for two phenethylamide metabolites isolated from marine Halobacillus salinus bacteria (Teasdale et al, 2009). In a more recent study, Noumi et al (2018) reported 69.3% of violacein inhibition by tea tree oil. Our study achieved a stronger inhibition of violacein production by 2,4-DBP that we isolated from D. eschscholtzii.…”

Section: 4-di-tert-butylphenol Was Identified As the Bioactive Antimentioning

confidence: 95%

2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

et al. 2020

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Pseudomonas aeruginosa is among the top three gram-negative bacteria according to the WHO's critical priority list of pathogens against which newer antibiotics are urgently needed and considered a global threat due to multiple drug resistance. This situation demands unconventional antimicrobial strategies such as the inhibition of quorum sensing to alleviate the manifestation of classical resistance mechanisms. Here, we report that 2,4-di-tert-butylphenol (2,4-DBP), isolated from an endophytic fungus, Daldinia eschscholtzii, inhibits the quorum-sensing properties of P. aeruginosa. We have found that treating P. aeruginosa with 2,4-DBP substantially reduced the secretion of virulence factors as well as biofilm, and its associated factors that are controlled by quorum sensing, in a dose-dependent manner. Concomitantly, 2,4-DBP also significantly reduced the expression of quorum sensing-related genes, i.e., lasI, lasR, rhlI, and rhlR significantly. Importantly, 2,4-DBP restricted the adhesion and invasion of P. aeruginosa to the A549 lung alveolar carcinoma cells. In addition, bactericidal assay with 2,4-DBP exhibited synergism with ampicillin to kill P. aeruginosa. Furthermore, our computational studies predicted that 2,4-DBP could bind to the P. aeruginosa quorumsensing receptors LasR and RhlR. Collectively, these data suggest that 2,4-DBP can be exploited as a standalone drug or in combination with antibiotic(s) as an anti-virulence and anti-biofilm agent to combat the multidrug resistant P. aeruginosa infection.

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Section: 4-di-tert-butylphenol Was Identified As the Bioactive Antimentioning

confidence: 95%

2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

et al. 2020

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“…The use of 60 μΜ cinnamaldehyde can decrease down to 55% the bioluminescence of V. harveyi BB886, which is induced by 3-hydroxy-C4-HSL, and from 60 to 100% that of V. harvevi BB170 (mediated by AI-2). This indicates, once again, that the activity of EOs, like all other phytochemicals, can be dependent even on the strain used within the same species [30,65,79,80], further than on the QS molecule involved. Another study showed that cinnamaldehyde particularly directs its action toward the short-chain AHL synthase (RhlI) and inhibits AHL production by RhlI [81].…”

Section: Effect Of Essential Oils On Microbial Cells (Modified Frommentioning

confidence: 98%

Essential Oils and Microbial Communication

Nazzaro¹,

Fratianni²,

d’Acierno³

et al. 2020

Essential Oils - Oils of Nature

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The World Health Organization highlighted the increase in the resistance to conventional antibiotics for most pathogens and observed also a decrease of the threshold for all mechanisms of cell-cell microbial communication, leading to the formation of biofilms and to the increase of microbial pathogenicity. Scientific community is therefore oriented to the identification and study of alternative substances to antibiotics. In such context, substances of vegetal source, such as essential oils (EOs), always used in traditional medicine, stimulated-particularly in recent decades-the scientific world to discover and identify substances, intended as a mixture or single components capable to fight pathogenic microorganisms. From this point of view, the study of plants is very interesting and offers many interesting ideas and results. This brief chapter describes the basis of the microbial communication, until the formation of biofilm, and some mechanisms through which essential oils, or some of their main components, may decrease or inactivate the complex mechanisms that lead to pathogenicity, both of prokaryotes and eukaryotes.

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“…For anti-QS experiments, several bacterial reporter assays with QS-regulated phenotypes (e.g., color pigments, bioluminescence) have been developed to identify chemical compounds that are able to interfere with or inhibit different bacterial autoinducer QS systems (e.g., AHL, AI-2, or AIP QS systems) [35][36][37][38][39][40][41][42]. In CVtype strains ATCC 12472 and ATCC 31532, the production of the purple pigment violacein is driven by different AHL autoinducers, whereas in the type strain CV026, deficient in AHL synthase, the production of violacein requires the exogenous addition of C6-AHL [42].…”

Section: Evaluation and Limitation Of Literaturementioning

confidence: 99%

Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Reichling

2020

Planta Med

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Pathogenic biofilm-associated bacteria that adhere to biological or nonbiological surfaces are a big challenge to the healthcare and food industries. Antibiotics or disinfectants often fail in an attempt to eliminate biofilms from those surfaces. Based on selected experimental research, this review deals with the potential biofilm-inhibiting, virulence factor-reducing, and biofilm-eradicating activities of essential oils and single essential oil compounds using Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Chromobacterium violaceum as model organisms. In addition, for the bacteria reviewed in this overview, different essential oils and essential oil compounds were reported to be able to modulate the expression of genes that are involved in the formation of autoinducer molecules, biofilms, and virulence factors. The anti-quorum sensing activity of some essential oils and single essential oil compounds was demonstrated using the gram-negative bacterium C. violaceum. Reporter strains of this bacterium produce the violet-colored compound violacein whose synthesis is regulated by quorum sensing autoinducer molecules called acylhomeserinlactones. Of great interest was the discovery that enantiomeric monoterpenes affected the quorum sensing regulation system in different ways. While the (+)-enantiomers of carvone, limonene, and borneol increased violacein formation, their (−)-analogues inhibited violacein production.For the successful eradication of biofilms and the bacteria living inside them, it is absolutely necessary that the lipophilic volatile substances can penetrate into the aqueous channels of biofilms. As shown in recent work, hydrophilic nano-delivery systems encapsulating essential oils/essential oil compounds with antibacterial effects may contribute to overcome this problem.

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Chromobacterium violaceum and Pseudomonas aeruginosa PAO1: Models for Evaluating Anti-Quorum Sensing Activity of Melaleuca alternifolia Essential Oil and Its Main Component Terpinen-4-ol

Cited by 60 publications

References 51 publications

2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

2,4-Di-Tert-Butylphenol Isolated From an Endophytic Fungus, Daldinia eschscholtzii, Reduces Virulence and Quorum Sensing in Pseudomonas aeruginosa

Essential Oils and Microbial Communication

Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

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