Chromatography Paper Strip Method for Collection, Transportation, and Storage of Rotavirus RNA in Stool Samples

Rahman, Mohammad Anisur; Goegebuer, Truus; Leener, Karolien De; Maes, Piet; Matthijnssens, Jelle; Podder, Goutam; Azim, Tasnim; Ranst, Marc Van

doi:10.1128/jcm.42.4.1605-1608.2004

Cited by 22 publications

(18 citation statements)

References 21 publications

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“…Previously we showed that pathogenic bacteria such as Vibrio cholerae , enterotoxigenic E. coli , enteropathogenic E. coli , Salmonelle typhimurium and Shigella dysenteriae , were not able to survive on the SDS/EDTA strips [16]. The biosafety test performed in this study demonstrated that adenoviruses also lost infectivity upon contact with the SDS/EDTA strips (Figure 2).…”

Section: Resultsmentioning

confidence: 55%

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Zlateva

Maes

Rahman

et al. 2005

Virol J

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Background: The enteric subgroup F adenoviruses type 40 (Ad40) and 41 (Ad41) are the second most important cause of acute infantile gastroenteritis after rotaviruses. Repeated community outbreaks have been associated with antigenic changes among the Ad40 and Ad41 strains due to host immune pressure. Therefore large field epidemiological surveys and studies on the genetic variations in different isolates of Ad40 and Ad41 are important for disease control programs, the design of efficient diagnostic kits and vaccines against subgroup F adenoviruses. A novel method using sodium dodecyl sulphate SDS/EDTA-pretreated chromatography paper strips was evaluated for the collection, storage and shipping of Ad40/41 contaminated stool samples.

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Section: Resultsmentioning

confidence: 55%

“…Filter paper sampling has been successfully used for screening studies of rotaviruses [16], noroviruses [17], human herpesviruses 6 and 7 [14], human immunodeficiency virus [13,18], hepatitis C virus [19], measles virus [20] and others viruses.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Zlateva

Maes

Rahman

et al. 2005

Virol J

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“…Fecal samples containing RVA were collected and shipped to the Rega Institute for Medical Research using chromatography paper strips as previously described [39].…”

Section: Methodsmentioning

confidence: 99%

Rotavirus Surveillance in Kisangani, the Democratic Republic of the Congo, Reveals a High Number of Unusual Genotypes and Gene Segments of Animal Origin in Non-Vaccinated Symptomatic Children

et al. 2014

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Group A rotavirus (RVA) infections form a major public health problem, especially in low-income countries like the Democratic Republic of the Congo (COD). However, limited data on RVA diversity is available from sub-Saharan Africa in general and the COD in particular. Therefore, the first aim of this study was to determine the genetic diversity of 99 RVAs detected during 2007–2010 in Kisangani, COD. The predominant G-type was G1 (39%) and the most predominant P-type was P[6] (53%). A total of eight different G/P-combinations were found: G1P[8] (28%), G8P[6] (26%), G2P[4] (14%), G12P[6] (13%), G1P[6] (11%), G9P[8] (4%), G4P[6] (2%) and G8P[4] (1%). The second aim of this study was to gain insight into the diversity of P[6] RVA strains in the COD. Therefore, we selected five P[6] RVA strains in combination with the G1, G4, G8 (2x) or G12 genotype for complete genome analysis. Complete genome analysis showed that the genetic background of the G1P[6] and G12P[6] strains was entirely composed of genotype 1 (Wa-like), while the segments of the two G8P[6] strains were identified as genotype 2 (DS-1-like). Interestingly, all four strains possessed a NSP4 gene of animal origin. The analyzed G4P[6] RVA strain was found to possess the unusual G4-P[6]-I1-R1-C1-M1-A1-N1-T7-E1-H1 constellation. Although the majority of its genes (if not all), were presumably of porcine origin, this strain was able to cause gastro-enteritis in humans. The high prevalence of unusual RVA strains in the COD highlights the need for continued surveillance of RVA diversity in the COD. These results also underline the importance of complete genetic characterization of RVA strains and indicate that reassortments and interspecies transmission among human and animal RVAs strains occur regularly. Based on these data, RVA vaccines will be challenged with a wide variety of different RVA strain types in the COD.

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“…RV strains DRC88 and DRC86 were collected from patients in the Democratic Republic of Congo in 2003, during an outbreak of acute diarrhea among young children under 5 years of age, at the Kalembe-Lembe Pediatric hospital in Kinshasa, DRC. Fecal samples containing these RV isolates were collected at the Institut National de Recherche Bio-Medicale and were transported to the Rega Institute, University of Leuven, Belgium, using chromatography paper strips (54).…”

Section: Methodsmentioning

confidence: 99%