Abstract:The fractionation of nucleic acids by chromatography on hydroxyapatite has been studied. The products, formed by annealing T2 DNA with complementary RNA, were separated in a linear gradient of phosphate buffer into three fractions containing, respectively, in their order of elution, single-stranded DNA, RNA complexed mainly with single-stranded DNA, and renatured DNA complexed with smaller amounts of RNA. By using annealing conditions which favour hybrid formation the complementary strand of DNA can be separat… Show more
“…(Log Me, is the appropriate quantity to use when comparing elution molarities, Martinson, 1973b. ) The chromatographic behaviors of some types of DNA-RNA hybrid molecules on hydroxylapatite have been reported (see in particular Becker et al, 1970, andSiebke andEkren, 1970). However the hybrids of Becker et al (1970) contained only 25 % or less RNA and the studies of Siebke and Ekren (1970) involved T2 phage DNA which chromatograms atypically on hydroxylapatite (Oishi, 1971).…”
Section: Resultsmentioning
confidence: 99%
“…) The chromatographic behaviors of some types of DNA-RNA hybrid molecules on hydroxylapatite have been reported (see in particular Becker et al, 1970, andSiebke andEkren, 1970). However the hybrids of Becker et al (1970) contained only 25 % or less RNA and the studies of Siebke and Ekren (1970) involved T2 phage DNA which chromatograms atypically on hydroxylapatite (Oishi, 1971). Therefore the chromatographic characteristics of their structures cannot be taken as representative of hybrid helices in general.…”
Section: Resultsmentioning
confidence: 99%
“…The buoyant density of this RNA in Cs2S04 (5-ml gradients were centrifuged in a Spinco 40.3 rotor at 40,000 rpm for 3 days at 20°) was found to be identical with that of CPV ds-RNA when the two were run together and is about 1.605 g/cm3. The base composition of both RNAs is 43% G 1 C (Sinsheimer, 1959;Miura et al, 1968).…”
“…(Log Me, is the appropriate quantity to use when comparing elution molarities, Martinson, 1973b. ) The chromatographic behaviors of some types of DNA-RNA hybrid molecules on hydroxylapatite have been reported (see in particular Becker et al, 1970, andSiebke andEkren, 1970). However the hybrids of Becker et al (1970) contained only 25 % or less RNA and the studies of Siebke and Ekren (1970) involved T2 phage DNA which chromatograms atypically on hydroxylapatite (Oishi, 1971).…”
Section: Resultsmentioning
confidence: 99%
“…) The chromatographic behaviors of some types of DNA-RNA hybrid molecules on hydroxylapatite have been reported (see in particular Becker et al, 1970, andSiebke andEkren, 1970). However the hybrids of Becker et al (1970) contained only 25 % or less RNA and the studies of Siebke and Ekren (1970) involved T2 phage DNA which chromatograms atypically on hydroxylapatite (Oishi, 1971). Therefore the chromatographic characteristics of their structures cannot be taken as representative of hybrid helices in general.…”
Section: Resultsmentioning
confidence: 99%
“…The buoyant density of this RNA in Cs2S04 (5-ml gradients were centrifuged in a Spinco 40.3 rotor at 40,000 rpm for 3 days at 20°) was found to be identical with that of CPV ds-RNA when the two were run together and is about 1.605 g/cm3. The base composition of both RNAs is 43% G 1 C (Sinsheimer, 1959;Miura et al, 1968).…”
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