Role of double-stranded nucleic acid backbone configuration in adsorption interactions

Martinson, Harold G.

doi:10.1021/bi00738a029

Cited by 30 publications

(8 citation statements)

References 72 publications

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“…Attempts to extract DNA from several smooth interpore regions on the outer surface consistently failed to yield amplifiable DNA, in direct contrast to extractions that included pore-rich regions. We suggest that cells sloughed from the laying or incubating bird are collected in the pores and are effectively held there as a result of the known tight interaction of calcium from the eggshell with the DNA backbone (27).…”

Section: Reference Sequencementioning

confidence: 98%

Ancient DNA reveals extreme egg morphology and nesting behavior in New Zealand’s extinct moa

Huynen

Gill²,

Millar

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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New Zealand's extinct flightless moa radiated rapidly into a large number of morphologically diverse species, which produced an equally large range of egg morphologies. The exact number of moa species, as well as the characteristics of the eggs they laid, remains contentious. Moreover, like most extinct species, we understand little about their nesting and incubation habits. We used a modified ancient DNA extraction procedure to recover exogenous mitochondrial and nuclear DNA from the inside and outside surfaces of moa eggs. We used sequences from the inside of 69 eggshells to directly assign these remains to seven of the 10 currently recognized moa species. In addition we were able to assign, to the species level, six of the rare reconstructed "whole" eggs. These molecular results enabled us to identify two distinct lineages within the genus Euryapteryx. Members of these lineages differed in eggshell thickness, with one lineage being characterized by a relatively thin eggshell. Unexpectedly, several thin-shelled eggs were also shown to belong to the heaviest moa of the genera Dinornis, Euryapteryx and Emeus, making these, to our knowledge, the most fragile of all avian eggs measured to date. Moreover, sex-specific DNA recovered from the outer surfaces of eggshells belonging to species of Dinornis and Euryapteryx suggest that these very thin eggs were likely to have been incubated by the lighter males. The thin nature of the eggshells of these larger species of moa, even if incubated by the male, suggests that egg breakage in these species would have been common if the typical contact method of avian egg incubation was used.avian eggs | exogenous DNA | endogenous DNA | ratite | evolution N ew Zealand's extinct moa (Aves: Dinornithiformes) were ratite birds. Living species of ratites include the emu and cassowary of Australia and New Guinea, the kiwi of New Zealand, and the rhea of South America. Extinct members include the elephant bird of Madagascar as well as approximately 10 species of moa (1-4). Moa were flightless and were particularly diverse in both size and in morphology (1, 2, 4-8). For example, members of the genus Dinornis were characterized by large body size, weighing as much as 250 kg, with extreme sexual dimorphism in which females were approximately twice the size of males (2, 5). In contrast, individuals of the coastal moa Euryapteryx curtus weighed as little as 9 kg (4) and females were approximately 20% larger than males. As a group, moa represented one of the most dramatic examples of morphological radiation in the history of vertebrates (1, 2, 6) and have been of international interest since originally described by Richard Owen in 1839 (9). This remarkable ratite group vanished soon after the settlement of New Zealand by Polynesians late in the 13th century (8, 10).Many questions about moa biology and evolution remain, despite more than 100 y of scientific investigation. Moa eggs ranged from 10 times the volume of a standard chicken egg (approximately 120 mm × 95 mm; ∼0.06 kg) to more than 8...

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Section: Reference Sequencementioning

confidence: 98%

Ancient DNA reveals extreme egg morphology and nesting behavior in New Zealand’s extinct moa

Huynen

Gill²,

Millar

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…DNA size has an intense effect on its adsorption as size may affect the conformational restriction and ability to nd available binding sites on surfaces. 34,35 Electrostatic binding normally appears in cationic molecules that are not capable to bind to the internal pockets of DNA. Therefore, our modied Hoechst MNPs interact with DNA along the exterior of the helix, and the complex is dominated by coulomb interactions between cationic species and the positively charged phosphate backbone.…”

Section: Plasmid and Ssdna Adsorption Behaviorsmentioning

confidence: 99%

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

et al. 2017

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“…Hydroxyapatite (HTP) has been used for many years as a high-performance liquid chromatography column matrix to bind and separate nucleic acids and proteins (1,6,7). Different types of DNA molecules as well as different types of RNA can be separated with HTP (2, 7).…”

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confidence: 99%

Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method

Purdy¹,

Embley²,

Takii³

et al. 1996

Appl Environ Microbiol

158

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Role of double-stranded nucleic acid backbone configuration in adsorption interactions

Cited by 30 publications

References 72 publications

Ancient DNA reveals extreme egg morphology and nesting behavior in New Zealand’s extinct moa

Ancient DNA reveals extreme egg morphology and nesting behavior in New Zealand’s extinct moa

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method

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