Chromatography of Biological Materials on Polyethylene Glycol Treated Controlled-Pore Glass

Hawk, Gerald L.; Cameron, J. A.; Baldwin, Larry

doi:10.1080/00327487208061469

Cited by 58 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The coelution of the virus at the column Vo was expected, since the mean pore diameter of the CPG was seleeted to exclude particles with diameters greater than about 750 A_ (5,7,9). The slight inclusion of bovine serum albumin into the column was unexpected and may reflect the erratic etution properties of CPG eotumn packings when soluble proteins are sieved through this type of chromatographic medium (9).…”

Section: Column E//iciency Evaluationmentioning

confidence: 96%

See 1 more Smart Citation

The rapid concentration and purification of influenza virus from allantoic fluid

Heyward¹,

Klimas²,

Stapp³

et al. 1977

Archives of Virology

View full text Add to dashboard Cite

Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.

show abstract

Section: Column E//iciency Evaluationmentioning

confidence: 96%

“…The slight inclusion of bovine serum albumin into the column was unexpected and may reflect the erratic etution properties of CPG eotumn packings when soluble proteins are sieved through this type of chromatographic medium (9).…”

Section: Column E//iciency Evaluationmentioning

confidence: 99%

The rapid concentration and purification of influenza virus from allantoic fluid

Heyward¹,

Klimas²,

Stapp³

et al. 1977

Archives of Virology

View full text Add to dashboard Cite

show abstract

“…PEO surfaces have but for other areas as well, such as membranes for been prepared by physical adsorption of PEO onto exseparation processes, biosensors, chromatographic isting substrates. [18][19][20][21] Only high-molecular-weight PEO supports, contact lenses, immunoassay, blood, and (mol. wt.…”

Section: Introductionmentioning

confidence: 99%

Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces

Lee¹,

Jeong

Lee

1997

J. Biomed. Mater. Res.

146

View full text Add to dashboard Cite

Comb-like polyethylene oxide (PEO) surfaces were prepared on low-density polyethylene (PE). The comb-like PEO chain density was changed gradually along the sample lengths by corona discharge treatment with gradually increasing power and the following graft copolymerization of poly(ethylene glycol) monomethacrylate macromers (PEO-MA). The macromers with different PEO repeat unit, 1, 5, and 10, were used. The prepared comb-like PEO gradient surfaces were characterized by water contact angle, Fourier transform infrared spectroscopy in the attenuated total reflectance mode, and electron spectroscopy for chemical analysis. All these measurements indicated that the PEO chains are grafted on the PE surface with gradually increasing density of PEO. Plasma protein adsorption and platelet adhesion on the PEO gradient surfaces decreased with increasing PEO chain length and surface density. As observed by scanning electron microscopy, PEO10-MA-grafted surface with high PEO density was very effective in preventing protein adsorption and platelet adhesion and did not activate the platelets.

show abstract

“…A series of 25, 50, and 250 ml beakers (Pyrex™ glass and Teflon®) were examined as well as 20 ml plastic high-density polyethylene (HDPE) vials and treated and non-treated borosilicate glass scintillation vials. One set of glass scintillation vials was treated with an aqueous 5% polyethylene glycol (PEG; 20,000 MW; Avocado Research Chemicals Ltd.; Heysham, UK) solution according to Hawk et al (1972). Vials were filled with the PEG solution for 5 min, drained, and then dried at 120°C overnight under vacuum.…”

mentioning

confidence: 99%

Influence of container adsorption upon observed pyrethroid toxicity to Ceriodaphnia dubia and Hyalella azteca

Wheelock

Miller²,

Miller³

et al. 2005

Aquatic Toxicology

View full text Add to dashboard Cite

Pyrethroid insecticides are known for their potential toxicity to aquatic invertebrates and many fish species. A significant problem in the study of pyrethroid toxicity is their extreme hydrophobicity. They can adsorb to test container surfaces and many studies, therefore, report pyrethroid levels as nominal water concentrations. In this study, pyrethroid adsorption to sampling and test containers was measured and several container treatments were examined for their ability to decrease pyrethroid adsorption. None of the chemical treatments were successful at preventing pyrethroid loss from aqueous samples, but vortexing of containers served to resuspend pyrethroids. The effects of the observed adsorption on Ceriodaphnia dubia and Hyalella azteca permethrin toxicity were examined. Species-specific results showed a time-dependent decrease in toxicity following pyrethroid adsorption to test containers for C. dubia, but not for H. azteca. These results demonstrate that pyrethroid adsorption to containers can significantly affect the observed outcome in toxicity-testing and serves as a caution for researchers and testing laboratories.

show abstract

Chromatography of Biological Materials on Polyethylene Glycol Treated Controlled-Pore Glass

Cited by 58 publications

References 12 publications

The rapid concentration and purification of influenza virus from allantoic fluid

The rapid concentration and purification of influenza virus from allantoic fluid

Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces

Influence of container adsorption upon observed pyrethroid toxicity to Ceriodaphnia dubia and Hyalella azteca

Contact Info

Product

Resources

About