Chromatographic study of the structural properties of mesoporous silica layers deposited on radially elongated pillars

On the potential use of two-photon polymerization to 3D print chromatographic packed bed supports

“…The presently printed material furthermore lacks any mesoporosity, such that an additional cladding step with a meso-porous layer via e.g., sol-gel deposition, is mandatory. In a first approach, one could start from an 80% TSM with ds,CAD=1.5 µm and clad it with a 250 nm mesoporous shell layer to obtain a 65% TSM [76]. Given the need to flush the structure with a very viscous sol, the cladding process can however be expected to be very cumbersome and prone to column-to-column variations.…”

Chromatographic separation of peptides and proteins for characterization of proteomes

“…To increase the retentive retention capability and sample loadability, Desmet and co-workers prepared organic-silica hybrid mesopore layers on pillars. 127,128 In a gradient mode, peak capacities of 160 (for the digests of cytochrome c, t G = 150 min) were obtained. 129 Nowadays, mPACs columns are commercially available as PharmaFluidics mPACt columns and have been applied to the sensitive profiling of low-input proteomics.…”

Challenges in Obtaining Relevant Information from One- and Two-Dimensional LC Experiments

“…The current limitation to adapting OT-LC and pillar array columns to portable LC is the decrease in sample loadability due to reduced stationary phase surface area. Although this area can be increased by adding porous monolithic structures onto fused silica walls (70) or etching the surfaces of pillar structures to increase porosity (71), detection modes that are most amenable to lower sample concentrations are still the main choice when using these types of columns. Mass spectrometry (MS) is often utilized, but there are a significant number of challenges when trying to reduce these large MS instruments to hand-portable formats (72).…”