Chromatographic separation of enantiomers

Abstract

In this paper a review is presented on the chromatographic analysis of enantiomers with special attention to high pressure liquid chromatography. Also, some examples of resolution of racemates by thin layer chromatography and gas chromatography are given. The various procedures in the surveyed literature have been divided into three main classes: procedures with formation of diastereomeric compounds prior to the chromatographic separation, procedures in which a chiral mobile phase is used, and procedures with the use of a chiral stationary phase. These methods are subdivided and some examples of their application to drugs and related compounds are presented.

Stereoselectivity in clinical pharmacokinetics and drug development

“…There are many books (19)(20)(21)(22)(23) and reviews (24)(25)(26)(27)(28)(29)(30) written on the subject Analysis of separate enantiomers requires there to be a chiral environment which in some way recognises or can separate enantiomers which are normally physicochemically identical. There are a number of techniques which can achieve this: A) chiral reagents; B) chiral stationary phases (CSP); C) chiraI mobile phases; D) capillary zone electrophoresis (CZE); E) chiral detectors; F) chiraI immuno or radio receptor assays.…”