Resolution of enantiomers of amino acids by HPLC

Abstract

Application of HPLC as a prime tool in the area of enantiomeric resolution has opened doors of success and varied interest. Use of chiral reagents either indirectly (as derivatization reagent) or directly (added to stationary or mobile phase) has led to achieve resolution of a wide range of compounds. Amino acids, being important molecules with simple structure and easy availability, have been extensively studied. A bibliographic survey on HPLC resolution of amino acids and derivatives along with a brief discussion on general methods of enantiomeric separation has been presented.

Chiral separation by chromatographic and electromigration techniques. A Review

“…Specialized reviews report on the use of chiral derivatization reagents for amino acids [18], drug enantiomers bearing hydroxy groups [19], neurotransmitters [20], amphetamine analogues [21] and for biomedical chromatography in general [22]. An excellent overview of fluorescent chiral derivatization reagents has been given by Toyo'oka [23].…”

Direct enantiomeric separations by high performance liquid chromatography using cyclodextrins

“…Thus, they may have very different interactions with a given pair of enantiomers. Several review articles on enantiomeric separation by HPLC have been published (Mehta, 1988;Krstulovic, 1989a, b;Ahuja, 1991;Pasutto, 1992;Taylor and Maher, 1992;Bhushan and Joshi, 1993;Witte, 1993;Subert, 1994).…”

Chiral Analysis of Amino Acids Using Electrochemical Composite Bienzyme Biosensors

“…Regarding chiral analysis of amino acids, a chiral derivatizing reagent can be used to form diastereomeric derivatives which can be separated by chromatography on a stationary phase (7). Furthermore, chiral stationary phases or chiral mobil phases have been also used to separate L-and D-amino acids (13). Another approach for the enantiomeric analysis of amino acids relies on the enzymatic reactions of L-and D-AAOD.…”