Chromatographic Profiles Analysis of Fruits ofCrataegusL. Genus by High-Performance Thin-Layer Chromatography

Abstract

It was known that hawthorn - Crataegus L. is a polymorphic genus. Two hawthorn species and their hybrids are included in the European Pharmacopoeia, twelve – in Ukrainian pharmacopoeia. Determination of chromatographic profiles of hawthorn fruits species native to Ukraine and other countries that are non-pharmacopoeial, but have sufficient plant raw material base, is essential for quality control of drugs. Aim. To analyze and compare the chromatographic profiles of fruits of 23 Crataegus L. species on phenolic compounds, evaluated by means of high-performance thin-layer chromatography procedure (HPTLC), and determine the specific features of chromatographic fingerprints. Materials and Methods. A total of 39 samples of fruits of 23 hawthorn species that are native to Europe, Asia and North America, such as Crataegus monogyna, C. laevigata/C. oxyacantha, C. leiomonogyna, C. curvisepala, C. pseudokyrtostyla, C. fallacina, C. subrotunda, C. ambigua, C. pentagyna, C. sanguinea, C. chlorosarca, C. almaatensis, C.pseudoheterophylla subsp. turkestanica, C. pinnatifida, C. pentagyna subsp. pseudomelanocarpa, C. punctata, C. pringlei, C. festiva, C. douglasii, C. holmesiana, C. submollis, C. flabellata, C. canadensis were investigated. The analysis has been done following the TLC method from European Pharmacopeia modified into HPTLC, using automated HPTLC herbal system (CAMAG, Switzerland). The results have shown that chromatographic profiles of phenolic constituents of nine Crataegus L. species of Europe, both pharmacopoeial and non-pharmacopoeial, were quite similar, despite the significant morphological distinctions. The chromatographical profiles of three species of Asia were similar to the pharmacopoeial species; three other species looked different and had specific marker zones. In addition, eight Crataegus L. species of North America had specific markers helping for discriminative analysis from pharmacopoeial species. Conclusion. The findings could help to identify the possible adulterations and prevent the falsification of finished products. The results will be taken into consideration during revision of the Ukrainian national pharmacopoeial monograph for hawthorn fruits.

Comprehensive Analysis of the Chemical and Bioactivity Profiles of Endemic Crataegus turcicus Dönmez in Comparison with Other Crataegus Species

“…On the other hand, in the current study, hydroalcoholic extracts of C. monogyna leaves and flower-bearing branches were found to contain hyperoside, chlorogenic acid, and rutin. In addition, Khokhlova et al [ 29 ] analyzed methanolic fruit extracts of different Crataegus species by HPTLC. As in the present study, they detected rutin, hyperoside, and chlorogenic acid both in C. monogyna and C. pentagyna.…”

Comparative study the essential oil composition of flowers and leaves of Crataegus monogyna L.

“…Preparations based on hawthorn herbal drugs are used both alone and in combination with other drugs and more pronounced antihypertensive activity is characteristic for hawthorn fruit extracts [5,6]. Alcoholic extracts of flowers are used in tachycardia and as coronary agents [7]. Known antioxidant properties of phenolic extracts of fruits and leaves of Crataegus monogyna L. [8,9].…”