Chromatographic performance of monodisperse macroporous particles produced by modified seeded polymerization. II. The effect of the diluent/seed‐latex ratio

Abstract

Poly(styrene-co-divinylbenzene)-based monodisperse macroporous particles were obtained by a modified seeded polymerization technique. The monodisperse polystyrene particles, obtained by dispersion polymerization in sufficiently large sizes and with suitable average molecular weights, were directly used as the seed latex in the production of macroporous particles. Therefore, the number of swelling and polymerization stages in the multistage production was reduced. In the first stage, the seed particles were swollen with a diluent, dibutyl phthalate (DBP), and then with a monomer phase including styrene, divinylbenzene, and, as an initiator, benzoyl peroxide. The monodisperse macroporous particles were obtained by the repolymerization of the monomer mixture in the seed particles. The particles, having different porosity characteristics, were synthesized through variations in the dibutyl phthalate/ seed-latex (DBP/SL) ratio. Selected macroporous particle samples were slurry-packed into stainless steel high-performance liquid chromatography columns (300 mm long ϫ 7.8-mm i.d.). The separation of the protein mixture by these columns in the reversed-phase chromatography (RPC) mode was investigated. Liquid chromatograms with high resolutions were obtained under an acetonitrile/water gradient over a wide range of flow rates (i.e., 0.5-3 mL/min), especially for the particles produced with a monomer/seedlatex (M/SL) ratio of 3.0 mL/g. In the RPC experiments, the particles produced with an M/SL ratio of 3.0 mL/g and DBP/SL ratios of 1.0 and 1.5 mL/g exhibited better chromatographic performance than the other samples. The maximum theoretical plate number was 3500 for the particles produced with the M/SL ratio of 3.0 mL/g and DBP/SL ratio of 1.5 mL/g with albumin as the analyte. The size exclusion chromatography (SEC) calibration curves and the back-pressure/flow-rate relationships of the produced columns were also determined. The particles obtained with an M/SL ratio of 3.0 mL/g and a DBP/SL ratio of 1.5 mL/g exhibited the best performance in SEC applications. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 92: [3685][3686][3687][3688][3689][3690][3691][3692][3693][3694][3695][3696] 2004

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

“…Poly(VAc-DVB) particles were slurry packed into 100 mm64.6 mm id stainless steel HPLC columns according to a protocol described elsewhere [24,25]. Chromatography was performed using a Shimadzu gradient liquid chromatograph (LC-10 ADVP) equipped with a SPD-10 AVVP UV detector.…”

Hydroxyl Functionalized Uniform‐Porous Beads, Synthesis and Chromatographic Use

“…The properties and suppliers of other chemical used in this study were given elsewhere (28,29). Corp. and used without further purification.…”

“…The isolated particles were washed with ethanol several times and extracted with tetrahydrofuran (THF) according to the procedure given elsewhere (28,29). The hydrolysis of poly(GMA-co-EDM) particles was performed in 0.5 M H 2 SO 4 solution at 608C for 3 h (13).…”

“…We tried a multistage polymerization protocol, the so called "modified seeded polymerization" in our laboratory. The uniform and porous poly(styrene-co-divinylbenzene) particles produced by "modified seeded polymerization" exhibited a good performance both in the gel permeation and reversed phase chromatography (28,29). In the next stage, the uniform porous particles carrying polar functional groups like hydroxyl, carboxyl, epoxypropyl and boronic acid were obtained (23 -27).…”

Synthese and characterization of boronic acid functionalized macroporous uniform poly(4-chloromethylstyrene-co-divinylbenzene) particles and its use in the isolation of antioxidant compounds from plant extracts

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