Chromatographic fingerprinting and quantitative analysis for the quality evaluation of Xinkeshu tablet

Abstract

A simple, sensitive and accurate method based on high performance liquid chromatography (HPLC) with diode array detector (DAD) was developed and validated for systematic quality evaluation of one type of traditional Chinese medicine preparations named Xinkeshu (XKS) tablet. In this study, the chromatographic fingerprints of XKS tablet were developed first, 23 peaks were selected as the common peaks to evaluate the similarities among different batches of XKS samples, which were manufactured in a long time span of three years. Additionally, simultaneous quantification of six markers in XKS tablet, including Danshensu, Protocatechuic aldehyde, Puerarin, Daidzin, Salvianolic acid B and Daidzein, was performed. The validation results showed that the developed method was specific, accurate, precise and robust. The preliminary explanation on why a close similarity between fingerprints did not exactly mean similar contents of chemical components in samples was given. The contribution of each chromatographic peak to similarity was also evaluated. The developed method offers an efficient, reliable and practical approach for systematic quality evaluation of XKS tablet.

Leaves of Invasive Plants—Japanese, Bohemian and Giant Knotweed—The Promising New Source of Flavan-3-ols and Proanthocyanidins

“…In Guidelines for the assessment of herbal medicines from 1991 WHO suggests chromatographic fingerprint for identification of a characteristic substance or mixture of substances when ensuring the consistent quality of plant preparations [20]. In addition, since 2004 the State Food and Drug Administration of China (SFDA) requires standardization by chromatographic fingerprints for all the injections made from herbal medicines or their raw materials [21]. Therefore, chromatographic fingerprinting of plant materials is often used as one of the key approaches of quality control of medicinal plants and plant materials used in the production of food supplements and different herbal (medicinal plants) preparations.…”

Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

“…Therefore, combination of the quantification of multicompounds and fingerprint analysis should be taken into consideration in the development of method for identifying and authenticating species. This combination has been used as a method of quality control for herbal products, from the raw materials to their related products (Wang et al 2012;Liu et al 2013;Liang et al 2014;Lv et al 2014). …”

Rapid determination of major bioactive isoflavonoid compounds during the extraction process of kudzu (Pueraria lobata) by near-infrared transmission spectroscopy

“…After appropriate dilution with 70% ethanol, the TIF was determined using UV spectroscopy reported by Qingxin Kong [25], and the validated reversed-phase HPLC assay [26] with a slight modification was applied to the quantitative determination of puerarin, daidzin and daidzein. Chromatographic analysis was performed on an Agilent 1260 HPLC system (Agilent Corp., Santa Clara, CA, USA).…”