Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns containing polar embedded groups/amino endcappings using principal component analysis

Euerby, Melvin R.; Petersson, Patrik

doi:10.1016/j.chroma.2004.10.027

Cited by 124 publications

(93 citation statements)

References 31 publications

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“…Euerby and Petersson [30] first proposed the hydrophilicity determination for characterization of stationary phases in HPLC, and used the value of the retention factor ratio between two sorbates that are differentiated by an OH group. They used for the hydrophilicity estimation the separation factor between benzyl alcohol and toluene.…”

Section: Hydrophilicitymentioning

confidence: 99%

“…The selectivity factor of these compounds is a measure for the steric selectivity. The selectivity factor of triphenylene/ortho-terphenyl [40], benzo[a]pyrene/tetrabenzonaphthalene [41] and benzoic acid/sorbic acid [30] is applied to characterize steric selectivity in RP HPLC.…”

Section: Steric Selectivitymentioning

confidence: 99%

“…Determination of the silanol activity of RP is a more difficult topic [42,43] in RP HPLC. The hydrogen bonding capacity in RPLC can be evaluated as the relative retention factors between a base and a neutral or a slightly acidic compound: caffeine and phenol, caffeine and o-terphenyl (Tanaka [3,40]); N,N-diethyl-m-toluamide and anthracene (Walters [4]); phenol and dimethylphthalate (Euerby and Petersson [30]); naphthalene and nitronaphthalene (Verzele and Dewaele [28]). …”

Section: Hydrogen Bonding Capacitymentioning

confidence: 99%

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Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography

Kulikov

Galat

2009

J of Separation Science

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Original PaperComparison of C18 silica bonded phases selectivity in micellar liquid chromatographyThe paper describes a new test designed in micellar LC (MLC) to compare the commercial C18 stationary phase properties. This test provides the total hydrophobicity, hydrophilicity, steric selectivity, hydrogen bonding, and ion-exchange capacity properties calculation of the ODS stationary phases. Both the test compounds and chromatographic separation conditions choice for column characterization in MLC are detailed. The chromatographic performance of several stationary phases that are used in MLC was evaluated with specific chromatographic test comprising nine test compounds, possessing different physico-chemical properties, which were injected on different supports with two micellar mobile phases: one at pH 7.0 (0.075 mol/L SDS and 1.5% v/v 1-pentanol), and other at pH 2.7 (0.075 mol/L SDS and 1.5% v/v 1-pentanol adjusted to pH by TFA). Fundamental column chromatographic properties were obtained under these conditions and were treated by hierarchical cluster analysis. From the results of cluster analysis, two closely related groups of columns are distinguished, and it was shown that the chosen column characteristic parameters allow characterizing both sorbent and micellar chromatographic system properties. Eleven columns were analyzed by this test, which allows a comparison of columns with the aim of the selection of suitable and analogous column for the analysis with MLC.

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Section: Hydrophilicitymentioning

confidence: 99%

Section: Steric Selectivitymentioning

confidence: 99%

Section: Hydrogen Bonding Capacitymentioning

confidence: 99%

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Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography

Kulikov

Galat

2009

J of Separation Science

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“…These groups provide hydrogen bonding interaction and thus can alter the selectivity for polar analytes, compared to pure hydrocarbon phases. More details about EPG phases can be found in the papers by Euerby and Petersson (2005) and Wilson et al (2004). 3.…”

Section: Reversed Phasementioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

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“…A recent trend in RPLC has been the introduction of stationary phases containing a polar functional group in addition to the nonpolar RP ligand [1][2][3][4][5][6][7][8]. In general, two types of RP stationary phases have been developed: polar endcapped phases, in which a polar group is used as an endcapping reagent after derivatization with a nonpolar ligand, and polar embedded group phases, in which a polar functional group is located within the alkyl chain itself.…”

Section: Introductionmentioning

confidence: 99%

Comparison of retention on traditional alkyl, polar endcapped, and polar embedded group stationary phases

Coym¹

2008

J of Separation Science

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Comparison of retention on traditional alkyl, polar endcapped, and polar embedded group stationary phasesThe development of new RP stationary phases containing polar groups has provided the chromatographer with a variety of stationary phase choices with differing selectivities. Polar endcapped and polar embedded group stationary phases have found use in solving a wide variety of separation problems, especially for the efficient separation of organic bases as well as separations necessitating the use of highly aqueous mobile phases. In this report, the retention thermodynamics of small, nonpolar solutes on traditional alkyl, polar endcapped, and polar embedded group stationary phases are compared. It is found that the nonpolar (methylene) transfer enthalpy is less favorable when polar embedded group phases are used, when compared to traditional or polar endcapped phases. In contrast, the transfer enthalpy of a phenyl group is found to be more favorable when a polar endcapped phase is used. In addition, the retention characteristics of these phases are compared using a set of solutes with differing solvatochromic parameters. Hydrogen-bond acids appear to have enhanced retention on polar embedded group phases, while hydrogen-bond bases have enhanced retention on polar endcapped phases.

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Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns containing polar embedded groups/amino endcappings using principal component analysis

Cited by 124 publications

References 31 publications

Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography

Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography

Theory and Practice of UHPLC and UHPLC–MS

Comparison of retention on traditional alkyl, polar endcapped, and polar embedded group stationary phases

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