Chromatographic Analysis of Acetylsalicylic Acid in Rhodococcus Cultural Fluids

Khrenkov, A. N.; Vikhareva, E. V.; Tumilovich, E.Y.; Karpenko, Yu. N.; Selyaninov, A. A.; Tyumina, Elena A.

doi:10.3103/s0027131420050053

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…On the seventh day of the experiment, a complete removal was observed only for meloxicam. Previously, we have shown the ability of rhodococci to completely biodegrade acetylsalicylic acid as the sole carbon and energy source and to co-metabolize diclofenac (with glucose), ketoprofen and ibuprofen (with n-hexadecane) [25][26][27][28]. Here, we confirmed for the first time the biodegradability of naproxen and meloxicam using Rhodococcus spp.…”

Section: Biodegradation Of Individual Nsaids and Induced Morphologica...supporting

confidence: 81%

“…Bacteria were cultivated in mineral salt medium, as described earlier [25]. The initial concentrations (100 mg/L) of medicinal substances (ibuprofen, meloxicam, and naproxen) were chosen based on their actual environmental concentrations detected in water and soil.…”

Section: Culture Conditionsmentioning

confidence: 99%

“…Earlier, we have first shown the ability of individual members of the genus Rhodococcus for complete biodegradation of analgesic and spasmolytic agents, including paracetamol [22], drotaverine [23,24], and acetylsalicylic acid [25], as well as highly toxic nonsteroidal antiinflammatory drugs (NSAIDs) diclofenac [26], ketoprofen [27], and ibuprofen [28].…”

Section: Introductionmentioning

confidence: 99%

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Cellular Modifications of Rhodococci Exposed to Separate and Combined Effects of Pharmaceutical Pollutants

et al. 2022

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Actinomycetes of the genus Rhodococcus (class Actinomycetia) are dominant dwellers of biotopes with anthropogenic load. They serve as a natural system of primary response to xenobiotics in open ecosystems, initiate defensive responses in the presence of pollutants, and are regarded as ideal agents capable of transforming and degrading pharmaceuticals. Here, the ability of selected Rhodococcus strains to co-metabolize nonsteroidal anti-inflammatory drugs (ibuprofen, meloxicam, and naproxen) and information on the protective mechanisms of rhodococci against toxic effects of pharmaceuticals, individually or in a mixture, have been demonstrated. For the first time, R. ruber IEGM 439 provided complete decomposition of 100 mg/L meloxicam after seven days. It was shown that versatile cellular modifications occurring at the early development stages of nonspecific reactions of Rhodococcus spp. in response to separate and combined effects of the tested pharmaceuticals included changes in electrokinetic characteristics and catalase activity; transition from unicellular to multicellular life forms accompanied by pronounced morphological abnormalities; changes in the average size of vegetative cells and surface area-to-volume ratio; and the formation of linked cell assemblages. The obtained data are considered as adaptation mechanisms in rhodococci, and consequently their increased resistance to separate and combined effects of ibuprofen, meloxicam, and naproxen.

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Section: Biodegradation Of Individual Nsaids and Induced Morphologica...supporting

confidence: 81%

Section: Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellular Modifications of Rhodococci Exposed to Separate and Combined Effects of Pharmaceutical Pollutants

et al. 2022

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“…It is worth noting, there is ever mounting number of the basic studies of stress-tolerant plant-associated microorganisms and plant-microbe interaction as well as applied aspects, particularly rhizoremediation, as an effective strategy for xenobiotic biodegradation (Vergani et al, 2019;González-Benítez Ivshina et al 10.3389/fmicb.2022.967127 Frontiers in Microbiology 19 frontiersin.org et al, Kuhl et al, 2021;Singha and Pandey, 2021;Zeng et al, 2022). R. josii IEGM 60 was isolated from oil-polluted soil and degraded acetylsalicylic acid (250 mg/L) in the form of a pharmaceutical substance and tablets within 11 and 6 days, respectively (Khrenkov et al, 2020). Recently, R. erythropolis IEGM 746 and R. rhodochrous IEGM 63 were selected as being capable of partial cometabolization of 100 mg/L ketoprofen and naproxen, respectively (Bazhutin et al, 2022;Ivshina et al, 2022).…”

Section: Nonsteroidal Anti-inflammatory Drugsmentioning

confidence: 99%

“…R . josii IEGM 60 was isolated from oil-polluted soil and degraded acetylsalicylic acid (250 mg/L) in the form of a pharmaceutical substance and tablets within 11 and 6 days, respectively ( Khrenkov et al, 2020 ). Recently, R .…”

Section: Biodegradation Of Pharmaceutical Pollutantsmentioning

confidence: 99%

Rhodococcus strains as a good biotool for neutralizing pharmaceutical pollutants and obtaining therapeutically valuable products: Through the past into the future

2022

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Active pharmaceutical ingredients present a substantial risk when they reach the environment and drinking water sources. As a new type of dangerous pollutants with high chemical resistance and pronounced biological effects, they accumulate everywhere, often in significant concentrations (μg/L) in ecological environments, food chains, organs of farm animals and humans, and cause an intense response from the aquatic and soil microbiota. Rhodococcus spp. (Actinomycetia class), which occupy a dominant position in polluted ecosystems, stand out among other microorganisms with the greatest variety of degradable pollutants and participate in natural attenuation, are considered as active agents with high transforming and degrading impacts on pharmaceutical compounds. Many representatives of rhodococci are promising as unique sources of specific transforming enzymes, quorum quenching tools, natural products and novel antimicrobials, biosurfactants and nanostructures. The review presents the latest knowledge and current trends regarding the use of Rhodococcus spp. in the processes of pharmaceutical pollutants’ biodegradation, as well as in the fields of biocatalysis and biotechnology for the production of targeted pharmaceutical products. The current literature sources presented in the review can be helpful in future research programs aimed at promoting Rhodococcus spp. as potential biodegraders and biotransformers to control pharmaceutical pollution in the environment.

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