Chromatin changes related to dedifferentiation and differentiation in tobacco tissue culture (Nicotiana tabacum L.)

Guerri, José; Culiáñez, Francisco; Primo‐Millo, Eduardo; Primo‐Yúfera, Eduardo

doi:10.1007/bf00429451

Cited by 9 publications

(2 citation statements)

References 16 publications

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“…That only a few proteins behave differently during the rapid and rather synchronous development of a large number of buds underlines that only a few major proteins are involved in the start of this process. Analogous results have been reported for a variety of developmental processes (BATES & CLELAND 1980, GUERRI et al 1982, MEYER et al 1984.…”

Section: Discussionsupporting

confidence: 66%

Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

Croes

Hesen

Loo

et al. 1986

Acta Botanica Neerlandica

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Protein synthesis was studied in relation to in vitro flower bud formation in tissue strips of tobacco. The rate of leucine incorporation increased from the 3rd day on up to the 7th day. Concomittantiy an increment in protein content was observed. Protein synthesis was slow in control explants which did not produce buds. From the first day onwards the nature of the polypeptides synthesized in vitrodiffered from that of the protein already present in the original explants. However, in spite of the progression of development towards bud formation, there are only a few changes in the complement of the proteins being synthesized during this 6-day period.

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Section: Discussionsupporting

confidence: 66%

Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

Croes

Hesen

Loo

et al. 1986

Acta Botanica Neerlandica

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“…Elements most closely involved in transcriptional events, hence in protein synthesis, are nonhistone chromosomal proteins; in fact, during dedifferentiation (pith explants to callus) and differentiation in Nicotiana tabncum, some high molecular weight bands (in SDS-PAGE) increase during an induction period to later decrease as cell division declines, while the opposite is true for a number of low M, components [58]. In Hordeum, several proteins are associated with dedifferentiation into callus of both embryo and root tissues, 24 independent of, and 8 relative to genotype or species [59].…”

Section: Discussionmentioning

confidence: 99%

Monitoring by two‐dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis

Gianazza¹,

Ponti²,

Scienza³

et al. 1992

Electrophoresis

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A two-dimensional electrophoretic analysis of the total proteins was carried out in Vitis rupestris as model system in order to characterize the different developmental stages--from callus to plantlets--of somatic embryogenesis events in the grapevine. The patterns of callus, embryogenetic callus, somatic embryos and plantlets derived from leaf and petiole explants were compared. Each differentiation step was characterized by specific peptide spots.

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Analysis of Polypeptides Associated With Shoot Formation in Tobacco Callus Cultures

Bertrand-Garcia

Walling

Murashige

1992

American J of Botany

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Callus lines of Nicotiana tabacum were selected for competence and lack of competence in shoot formation. Changes in total and chromosomal polypeptides in these shoot‐forming and nonshoot‐forming tobacco cultures were examined by twodimensional polyacrylamide gel electrophoresis. Qualitative and quantitative differences in total, nonhistone chromosomal, and basic chromosomal polypeptides were evident throughout the 7‐d test period. The analysis of total proteins identified polypeptides specific to shoot‐forming and nonshoot‐forming tissue during the 7‐d sampling period. A small number of basic chromosomal proteins were found solely in shoot‐forming or nonshoot‐forming tissue. One basic chromosomal protein was detected in only nonshoot‐forming tissue at all sampling times. Two proteins, although present in shoot‐forming tissue, were present at elevated levels in the nonshoot‐forming cultures. No temporal changes in basic proteins over the 7‐d incubation period were observed. Qualitative differences in total nonhistone chromosomal polypeptides in the shoot‐forming and nonshoot‐forming tissue were also observed. Differences in chromosomal polypeptides were observed. In contrast to the basic chromosomal proteins, temporal variation in the nonhistone chromosomal polypeptides was demonstrated. Throughout the 7‐d sampling period, 29 and 12 nonhistone chromosomal polypeptides varied qualitatively in shoot‐forming and nonshoot‐forming callus cultures, respectively. In vitro labeling with 32P‐orthophosphate indicated that approximately 1.0% and 0.3% of the nonhistone chromosomal proteins were phosphorylated in the shoot‐forming and nonshoot‐forming cultures. Of these phosphorylated polypeptides, one was present in nonshoot‐forming tissue and three were detected only in the shoot‐forming tissue. Phosphorylation occurred at serine or threonine residues.

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Chromatin changes related to dedifferentiation and differentiation in tobacco tissue culture (Nicotiana tabacum L.)

Cited by 9 publications

References 16 publications

Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

Monitoring by two‐dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis

Analysis of Polypeptides Associated With Shoot Formation in Tobacco Callus Cultures

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