Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

Croes, A. F.; Hesen, P. L. G. M.; Loo, A. A. J. van de; Barendse, G. W. M.

doi:10.1111/j.1438-8677.1986.tb01277.x

Acta Botanica Neerlandica

1986

DOI: 10.1111/j.1438-8677.1986.tb01277.x

|View full text |Cite

Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

A. F. Croes

P. L. G. M. Hesen

A. A. J. van de Loo

et al.

Abstract: Protein synthesis was studied in relation to in vitro flower bud formation in tissue strips of tobacco. The rate of leucine incorporation increased from the 3rd day on up to the 7th day. Concomittantiy an increment in protein content was observed. Protein synthesis was slow in control explants which did not produce buds. From the first day onwards the nature of the polypeptides synthesized in vitrodiffered from that of the protein already present in the original explants. However, in spite of the progression o… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1988

1990

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 14 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings also correlate well with those of Croes et al (7), who found, between d 3 and 7, de novo synthesis of proteins in cultures of thin-layer tobacco explants which differed from those already present in the explants. It is possible that such proteins arise by transglutaminase-mediated post-translational modification of existing protein, and that they are somehow associated with floral initiation.…”

supporting

confidence: 93%

Binding of Spermidine to a Unique Protein in Thin-Layer Tobacco Tissue Culture

Apelbaum¹,

Canellakis²,

Applewhite³

et al. 1988

Plant Physiol.

View full text Add to dashboard Cite

The mechanism by which spermidine induces the appearance of floral buds in thin-layer tobacco (Nicotiana tabacum) tissue culture was studied by following the fate of the radioactive compound. VHlSpermidine was taken up rapidly by the tissue, and after a brief lag, a portion was bound to trichloroacetic acid precipitable macromolecules. Such binding increased to a maximum on day 4 of culture, coinciding with the onset of bud differentiation, and declined thereafter until shortly before flowering. About 82% of the label in the trichloroacetic acid precipitate remained as spermidine, 14% was metabolized to putrescine, 3% to spermine, and 1% to 'y-aminobutyric acid. Spermidine was covalently bound to a protein with a molecular size of about 18 kilodaltons. Hydrolysis of this protein and analysis of the labeled entities revealed 81% spermidine, 16% putrescine, and 3% spermine. This post-translational modification of a unique protein by attachment of spermidine may be causally connected to the appearance of flower buds in thin-layer tobacco cultures.Polyamines are biologically ubiquitous and have been implicated in many aspects of growth and development in a wide variety of organisms (21)(22)(23) (8,9,16) and Vigna (11), the abnormal flowering habits of polyamine mutants of tobacco (13) After an 8 h period of incubation the tissue was ground in 10% TCA and the pellet washed with 5% TCA until washes were free of radioactivity. The TCA precipitate was then extracted with 0.1 M Na-phosphate (pH 6.8) containing 0.05% SDS, and the volume of the resulting solution reduced by an Amicon Centricon-10 concentrator. Aliquots were applied to a Varian TSK-2000 SW column, 60 cm x 7.5 mm. Elution with 0.1 M Na-phosphate (pH 6.8) occurred at a flow rate of 0.8 mL/ mm.Polyamines were extracted, danslyated, separated by TLC ( 11) or HPLC (3) and quantified using a spectrophotofluorimeter. The labeled TCA-soluble and -insoluble fractions were hydrolyzed in 6 N HCI at 1 10°C for at least 18 h prior to separation. Protein was determined following the method of Bradford (2).SDS-PAGE gels were run on a 5% stacking gel and a 14% separating gel of 0.5 mm thickness at 600 V for 2 h (15).

show abstract

supporting

confidence: 93%

Binding of Spermidine to a Unique Protein in Thin-Layer Tobacco Tissue Culture

Apelbaum¹,

Canellakis²,

Applewhite³

et al. 1988

Plant Physiol.

View full text Add to dashboard Cite

show abstract

The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco explants at a large range of concentrations

Smulders

Visser

Croes

et al. 1990

Planta

View full text Add to dashboard Cite

Short-term applications of very high concentrations of 1-naphthaleneacetic acid (NAA) to expiants from flower stalks of tobacco (Nicotiana tabacum L. cv. Samsun) induced flower-bud regeneration to the same extent as longer or continuous incubation on lower concentrations. The maximum number of flower buds per explant after 15 d of culture was obtained not only by continuous culturing at 1 μmol·l(-1) NAA but also by 12 h of culturing at 22 μmol·l(-1) or 0.5 h at 220 μmol· l(-1), followed by incubation on medium without auxin for the remaining period. Continuous application of such high concentrations resulted in callus formation or caused the death of the explanted tissue. In all experiments in which auxin concentration and time of application were independently varied, the product of concentration and time determined the number of buds formed. Most, but not all, of the NAA taken up by the tissues was converted into conjugates. In expiants which had received a dose which was optimal for regeneration, the internal concentration of free NAA remaining beyond the pulse period was between 1.7 and 6.2 μmol·l(-1). Suboptimal applications led to lower values, supraoptimal treatments to much higher internal concentrations. The physiological effect, which depends on the internal hormone concentration, thus manifested itself as dose-dependent with regard to applied hormone.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Protein Synthesis During in Vitro Flower Bud Formation in Tobacco

Cited by 2 publications

References 14 publications

Binding of Spermidine to a Unique Protein in Thin-Layer Tobacco Tissue Culture

Binding of Spermidine to a Unique Protein in Thin-Layer Tobacco Tissue Culture

The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco explants at a large range of concentrations

Contact Info

Product

Resources

About