Chromatic confocal microscopy with a novel wavelength detection method using transmittance

Kim, Taejoong; Kim, Sang Hoon; Do, Dukho; Yoo, Hongki; Gweon, Dae−Gab

doi:10.1364/oe.21.006286

Cited by 52 publications

(24 citation statements)

References 18 publications

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“…The experiments were carried out in such a way that the parameter d in Figure 3, which corresponded to the defocus of Detector2 from the focal plane of the fiber coupling lens, was changed from 0 µm to 300 µm in a step of 20 µm, while the focused wavelength at each step was being observed. In the experiments, the deviation of the parameter d (∆d) corresponded to −dF in Equation (15). It should be noted that the positive direction of the parameter d was set to be the one from the fiber detector to the fiber coupling lens.…”

Section: Fiber Coupling Lensmentioning

confidence: 99%

“…From the experimental results, the sensitivity dλ/dF was evaluated to be 0.5686 nm/μm. From Equation (15), the contribution of F in P4 was therefore evaluated as dZ/dT = (0.383 μm/°C)•(0.5686 nm/μm)•(−255 nm/nm) = −55.5 nm/°C. Figure 9a.…”

Section: Fiber Coupling Lensmentioning

confidence: 99%

“…This characteristic allows the chromatic confocal probe to reduce the influences of motion errors of the mechanical scan of a target sample. Conventional chromatic confocal probes employing a white light source such as a halogen lamp or an LED (light-emitting diode) have been achieved a sub-micrometric vertical resolution [13][14][15][16]. Meanwhile, instability and low spectral power density of such a white light source are issues to be addressed to achieve better performances.…”

Section: Introductionmentioning

confidence: 99%

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Investigation and Improvement of Thermal Stability of a Chromatic Confocal Probe with a Mode-Locked Femtosecond Laser Source

et al. 2019

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An intentional investigation on the thermal stability of a mode-locked femtosecond laser chromatic confocal probe, which is a critical issue for the probe to be applied for long-term displacement measurement or surface profile measurement requiring long-time scanning, is carried out. At first, the thermal instability of the first prototype measurement setup is evaluated in experiments where the existence of a considerably large thermal instability is confirmed. Then the possible reasons for the thermal instability of the measurement setup are analyzed quantitatively, such as the thermal instability of the refractive index of the confocal lens and the thermal expansion of mechanical jigs employed in the probe. It is verified that most of the thermal instability of the measurement setup is caused by the thermal expansion of mechanical jigs in the probe. For the improvement of the thermal stability of the probe, it is necessary to employ a low thermal expansion material for the mechanical jigs in the measurement setup and to shorten the optical path length of the laser beam. Based on the analysis result, a second prototype probe is newly designed and constructed. The improved thermal stability of the second prototype probe is verified through theoretical calculations and experiments.

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Section: Fiber Coupling Lensmentioning

confidence: 99%

Section: Fiber Coupling Lensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation and Improvement of Thermal Stability of a Chromatic Confocal Probe with a Mode-Locked Femtosecond Laser Source

et al. 2019

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“…The demand for accurately measuring the 3-dimensional (3-D) shape of various objects has been existing in many industrial and medical fields. Especially, the fast measurement technology of large-area and micro-surface is required in the inspection process of semiconductors, flat panel or plasma displays, and microelectromechanical systems [1][2][3]. In addition, large depth scanning range is needed in dental CAD/CAM industry [4].…”

Section: Introductionmentioning

confidence: 99%

High-speed 3-D measurement with a large field of view based on direct-view confocal microscope with an electrically tunable lens

2016

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We propose a new structure of confocal imaging system based on a direct-view confocal microscope (DVCM) with an electrically tunable lens (ETL). Since it has no mechanical moving parts to scan both the lateral (x-y) and axial (z) directions, the DVCM with an ETL allows for high-speed 3-dimensional (3-D) imaging. Axial response and signal intensity of the DVCM were analyzed theoretically according to the pinhole characteristics. The system was designed to have an isotropic spatial resolution of 20 µm in both lateral and axial direction with a large field of view (FOV) of 10 × 10 mm. The FOV was maintained according to the various focal shifts as a result of an integrated design of an objective lens with the ETL. The developed system was calibrated to have linear focal shift over a range of 9 mm with an applied current to the ETL. The system performance of 3-D volume imaging was demonstrated using standard height specimens and a dental plaster.

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“…Chromatic confocal microscopy uses a broadband light source and an objective with longitudinal chromatic aberrations, which generate multiple, axially-distributed foci depending on wavelength [15][16][17]. Chromatic confocal microscopy has been most widely developed using reflectance imaging for surface profiling, while several studies have investigated fluorescence and nonlinear optical imaging [18][19][20][21]. Differential confocal microscopy, which uses two or more detectors, is another method to measure surface shape with 2-D scans [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Dual-detection confocal fluorescence microscopy: fluorescence axial imaging without axial scanning

Lee¹,

Kim²,

Gweon³

et al. 2013

Opt. Express

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We propose a new method for high-speed, three-dimensional (3-D) fluorescence imaging, which we refer to as dual-detection confocal fluorescence microscopy (DDCFM). In contrast to conventional beam-scanning confocal fluorescence microscopy, where the focal spot must be scanned either optically or mechanically over a sample volume to reconstruct a 3-D image, DDCFM can obtain the depth of a fluorescent emitter without depth scanning. DDCFM comprises two photodetectors, each with a pinhole of different size, in the confocal detection system. Axial information on fluorescent emitters can be measured by the axial response curve through the ratio of intensity signals. DDCFM can rapidly acquire a 3-D fluorescent image from a single two-dimensional scan with less phototoxicity and photobleaching than confocal fluorescence microscopy because no mechanical depth scans are needed. We demonstrated the feasibility of the proposed method by phantom studies.

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Chromatic confocal microscopy with a novel wavelength detection method using transmittance

Cited by 52 publications

References 18 publications

Investigation and Improvement of Thermal Stability of a Chromatic Confocal Probe with a Mode-Locked Femtosecond Laser Source

Investigation and Improvement of Thermal Stability of a Chromatic Confocal Probe with a Mode-Locked Femtosecond Laser Source

High-speed 3-D measurement with a large field of view based on direct-view confocal microscope with an electrically tunable lens

Dual-detection confocal fluorescence microscopy: fluorescence axial imaging without axial scanning

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