Chromate-induced human erythrocytes haemoglobin oxidation and peroxidation: influence of vitamin E, vitamin C, salicylate, deferoxamine, and N-ethylmaleimide

Abstract

In order to attenuate or to prevent chromate-induced human erythrocytes injury, the influence of vitamin E, vitamin C, salicylate, deferoxamine, and N-ethylmaleimide on chromate-induced human erythrocytes haemoglobin oxidation and peroxidation were investigated. It was observed that pretreatment of human erythrocytes with vitamin E (20 mM), vitamin C (1 mM), salicylate (3 mM), and deferoxamine (4 mM) significantly increased (P =0.0001) chromate-induced human erythrocytes haemoglobin oxidation in a time dependent manner, while it was significantly decreased (P= 0.0001) by pretreatment with N-ethylmaleimide (1 mM). In contrast, pretreatment of human erythrocytes with deferoxamine (4 mM) immediately inhibited (P =0.0001) chromate-induced human erythrocytes peroxidation, while it was significantly increased (P= 0.0001) by pretreatment with N-ethylmaleimide (1 mM) during the first 4 h of cells exposition to chromate. For time periods superior to 6 h pretreatment with N-ethylmaleimide (1 mM) significantly decreased (P=0.0001) chromate-induced human erythrocytes peroxidation. It was concluded that care must be taken as these drugs are used to prevent against toxicity induced by chromium(VI) compounds.

Cr(III) exerts stronger structural effects than Cr(VI) on the human erythrocyte membrane and molecular models

“…It results amazing that after so many studies on chromium toxicity, and particularly the knowledge that Cr(VI) anions enter the erythrocytes readily [9,[26][27][28][29], there are practically no reports on the structural effects induced by chromium compounds to the erythrocyte membrane. In order to understand these effects Cr(III) under the form of CrCl 3 , and Cr(VI) as K 2 CrO 4 were incubated with intact erythrocytes, their isolated unsealed membranes (IUM) and bilayers built-up of DMPC and DMPE, phospholipid classes present in the outer and inner monolayers of the erythrocyte membrane, respectively.…”

Effects of ascorbate fatty ester derivatives on erythrocyte membrane lipoperoxidation

“…Besides, the exposition of RBCs to this kind of oxidative free radicals can produce membrane lipoperoxidation [9,25,[28][29][30] since RBCs membrane is enriched in poly-unsaturated fatty acids which are highly sensitive to oxidative stress [8,12]. Table 1 Rigidity indexes (RI) and TBARS (MDA production) in the presence of ASC 8 , ASC 10 10 10.1 ± 0.1 b 4.23 ± 0.2 a (ASC 8 ) CH 50 25.3 ± 0.3 b 5.14 ± 0.1 a (ASC 10 ) CMC 7.9 ± 0.1 c 3.2 ± 0.3 c (ASC 10 ) CH 10 16.5 ± 0.3 b 3.98 ± 0.4 a (ASC 10 ) CH 50 30.2 ± 0.2 b 4.83 ± 0.2 a (ASC 12 ) CMC 7.8 ± 0.1 c 3.18 ± 0.2 c (ASC 12 ) CH 10 11.5 ± 0.4 b 3.78 ± 0.3 a (ASC 12 ) CH 50 15.6 ± 0.5 b 4.68 ± 0.5 a CMC (critical micellar concentration); CH 10 (concentration producing 10% hemolysis); CH 50 (concentration producing 50% hemolysis). Data: mean ± SD, n = 14 (differences vs control medium: a p < 0.05, b p < 0.01, c ns).…”

“…The mixture was incubated for 15 minutes in a boiling water bath and cooled at room temperature. Then, the solution absorbance was measured at 532 nm, using 1,1,3,3tetraetoxipropano as standard for calibration curve [10]. The results were informed as nmol of MDA/ml of packed RBC.…”

Anti-Aging: Mythen und Fakten zur Diagnose und Therapie

“…Um 1900 war die allgemeine Lebenserwartung bei 49 Jahren und erfuhr bis 1997 einen Zuwachs von insgesamt 27 auf nunmehr 76 Jahre [4]. Von diesem enormen Lebenserwartungszuwachs von 27 Jahren in nur einem Jahrhundert entfällt der grösste Teil von 21 Jahren auf die ersten 70 Jahre [3] [51,52]. Dem «Dinner Cancelling», bei dem zwei bis drei Mal pro Woche ab 16 Uhr keine Nahrung mehr zu sich genommen werden sollte, wodurch eine nächtliche Hypoglykämie bewirkt wird, sowie einer allgemeinen Kalorieneinschränkung werden (unter anderem) ähnliche Effekte zugeschrieben.…”