Choosing an appropriate glucose concentration according to different cell types and experimental purposes is very important

Huang, Yan; Xiong, Zhi‐Gang

doi:10.1007/s12192-014-0547-y

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…Adherent neuronal cells are usually grown in the Dulbecco's Modified Eagle Medium (DMEM) containing 25 mM glucose, which here and in most of the studies dealing with neuroblasts represents a consolidated basal condition, because of the high metabolic rates of neuroblasts. [33] In a previous work, Liu et al reported that the exposure of hippocampal neurons to 50 mM glucose was enough to provoke evident damages to cells. [7] They verified that the osmolality of glucose solutions between 25 and 150 mM being between 260 and 320 mOsm/kg, did not exert any effect on cell normal metabolism.…”

Section: Discussionmentioning

confidence: 98%

Time-Dependent Influence of High Glucose Environment on the Metabolism of Neuronal Immortalized Cells

Colombaioni¹,

Campanella

Nieri³

et al. 2021

Preprint

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In this work the effect of glucose concentration on the metabolome of living hippocampal HN9.10e neurons was studied. This cell line represents a reliable, <i>in vitro </i>model of one of the most vulnerable regions of central nervous system. Targeted metabolites were analyzed in the cell culture medium by two direct methods, namely liquid chromatography – diode array detection and headspace – solid phase micro extraction – gas chromatography – mass spectrometry. Twenty-two metabolites were simultaneously identified and quantified in the growth medium of the cells, treated with 25, 50 or 75 mM glucose, sampled along 8 days to mimic a prolonged hyperglycemia. The results of statistical analysis showed the clear impairment of neuronal metabolism already after 48 hours, represented by a significant reduction of the metabolic activity.

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Section: Discussionmentioning

confidence: 98%

Time-Dependent Influence of High Glucose Environment on the Metabolism of Neuronal Immortalized Cells

Colombaioni¹,

Campanella

Nieri³

et al. 2021

Preprint

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“…For example, concentrations of glucose up to 150 mM (2.7%) for 24 h did not impair the viability of Schwann cells [65] and 55 mM for 24 h did not impair ganglion nerve cell viability [66]. This differential effect of glucose on various cell types has also been recognized in the required energy component (D-glucose) in culture mediums [67]. Differences in D-glucose concentration requirements appear to differ according to the normal physiological state of the cells in question.…”

Section: Discussionmentioning

confidence: 99%

The Potential of Glucose Treatment to Reduce Reactive Oxygen Species Production and Apoptosis of Inflamed Neural Cells In Vitro

Cherng,

Chang,

Tsai

et al. 2023

Biomedicines

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Neuroinflammation is a key feature in the pathogenesis of entrapment neuropathies. Clinical trial evidence suggests that perineural injection of glucose in water at entrapment sites has therapeutic benefits beyond a mere mechanical effect. We previously demonstrated that 12.5–25 mM glucose restored normal metabolism in human SH-SYFY neuronal cells rendered metabolically inactive from TNF-α exposure, a common initiator of neuroinflammation, and reduced secondary elevation of inflammatory cytokines. In the present study, we measured the effects of glucose treatment on cell survival, ROS activity, gene-related inflammation, and cell cycle regulation in the presence of neurogenic inflammation. We exposed SH-SY5Y cells to 10 ng/mL of TNF-α for 24 h to generate an inflammatory environment, followed by 24 h of exposure to 3.125, 6.25, 12.5, and 25 mM glucose. Glucose exposure, particularly at 12.5 mM, preserved apoptotic SH-SY5Y cell survival following a neuroinflammatory insult. ROS production was substantially reduced, suggesting a ROS scavenging effect. Glucose treatment significantly increased levels of CREB, JNK, and p70S6K (p < 0.01), pointing to antioxidative and anti-inflammatory actions through components of the MAPK family and Akt pathways but appeared underpowered (n = 6) to reach significance for NF-κB, p38, ERK1/2, Akt, and STAT5 (p < 0.05). Cell regulation analysis indicated that glucose treatment recovered/restored function in cells arrested in the S or G2/M-phases. In summary, glucose exposure in vitro restores function in apoptotic nerves after TNF-α exposure via several mechanisms, including ROS scavenging and enhancement of MAPK family and Akt pathways. These findings suggest that glucose injection about entrapped peripheral nerves may have several favorable biochemical actions that enhance neuronal cell function.

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“…The comparative molecular analyses showed that most other genes including Na + /K + /2Cl − cotransporter (NKCC), Na + /Cl − /HCO 3 − cotransporter (NBC), Na + /H + exchanger 3 (NHE3), Na + /Ca +2 exchanger 1 (NCX1), Arginine kinase (AK), Sarcoplasmic Ca +2 -ATase (SERCA) and Calreticulin (CRT) were conserved across the three Cherax crayfish ( Tables 3 and 4 ). The underlying reason may be that the these genes encode a set of enzymes that are actively or passively associated with the fundamental and common physiological processes of ion-regulation in crustaceans ( Lucu, 1989 ; Onken, Graszynski & Zeiske, 1991 ; Onken, Tresguerres & Luquet, 2003 ; Ahearn, Mandal & Mandal, 2004 ; Serrano, Halanych & Henry, 2007 ; Mandal et al, 2009 ; Lv et al, 2015 ; Ren et al, 2015 ; Xu et al, 2015 ). The high level of amino acid conservation in these predicted proteins may be attributed to the fact that they are found in important biochemical pathways that influence systemic acid–base balance and/or ion transport ( Uda et al, 2006 ; Hwang, Lee & Lin, 2011 ; Hiroi & McCormick, 2012 ; McNamara & Faria, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…These genes have previously been demonstrated to function as important enzymes involved in osmoregulatory organs such as gills and epipodites in a number of crustaceans ( Towle & Weihrauch, 2001 ; Weihrauch et al, 2004 ; Freire, Onken & McNamara, 2008 ; Mandal et al, 2009 ). In fact, gene expression data for Na + /K + /2Cl − cotransporter ( Luquet et al, 2005 ; Havird, Henry & Wilson, 2013 ) and Calreticulin ( Luana et al, 2007 ; Lv et al, 2015 ; Xu et al, 2015 ) show that they are induced under different ionic conditions and arginine kinase has also been shown to be induced under different pH and salinity conditions ( Serrano, Halanych & Henry, 2007 ; Xie et al, 2014 ; Ali et al, 2015b ).…”

Section: Discussionmentioning

confidence: 99%

Comparative molecular analyses of select pH- and osmoregulatory genes in three freshwater crayfishCherax quadricarinatus,C. destructorandC. cainii

Ali

Pavasovic

Dammannagoda

et al. 2017

PeerJ

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Systemic acid-base balance and osmotic/ionic regulation in decapod crustaceans are in part maintained by a set of transport-related enzymes such as carbonic anhydrase (CA), Na+/K+-ATPase (NKA), H+-ATPase (HAT), Na+/K+/2Cl− cotransporter (NKCC), Na+/Cl−/HCO cotransporter (NBC), Na+/H+ exchanger (NHE), Arginine kinase (AK), Sarcoplasmic Ca+2-ATPase (SERCA) and Calreticulin (CRT). We carried out a comparative molecular analysis of these genes in three commercially important yet eco-physiologically distinct freshwater crayfish, Cherax quadricarinatus, C. destructor and C. cainii, with the aim to identify mutations in these genes and determine if observed patterns of mutations were consistent with the action of natural selection. We also conducted a tissue-specific expression analysis of these genes across seven different organs, including gills, hepatopancreas, heart, kidney, liver, nerve and testes using NGS transcriptome data. The molecular analysis of the candidate genes revealed a high level of sequence conservation across the three Cherax sp. Hyphy analysis revealed that all candidate genes showed patterns of molecular variation consistent with neutral evolution. The tissue-specific expression analysis showed that 46% of candidate genes were expressed in all tissue types examined, while approximately 10% of candidate genes were only expressed in a single tissue type. The largest number of genes was observed in nerve (84%) and gills (78%) and the lowest in testes (66%). The tissue-specific expression analysis also revealed that most of the master genes regulating pH and osmoregulation (CA, NKA, HAT, NKCC, NBC, NHE) were expressed in all tissue types indicating an important physiological role for these genes outside of osmoregulation in other tissue types. The high level of sequence conservation observed in the candidate genes may be explained by the important role of these genes as well as potentially having a number of other basic physiological functions in different tissue types.

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Choosing an appropriate glucose concentration according to different cell types and experimental purposes is very important

Cited by 7 publications

References 11 publications

Time-Dependent Influence of High Glucose Environment on the Metabolism of Neuronal Immortalized Cells

Time-Dependent Influence of High Glucose Environment on the Metabolism of Neuronal Immortalized Cells

The Potential of Glucose Treatment to Reduce Reactive Oxygen Species Production and Apoptosis of Inflamed Neural Cells In Vitro

Comparative molecular analyses of select pH- and osmoregulatory genes in three freshwater crayfishCherax quadricarinatus,C. destructorandC. cainii

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