Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study)

Sumarta, Ni Putu Mira; D, Coen Pramono; Hendrianto, Eryk; Susilowati, Helen; Karsari, Deya; Rantam, Fedik Abdul

doi:10.15562/bmj.v5i3.295

Cited by 6 publications

(8 citation statements)

References 10 publications

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“…PRF administration in osteogenic culture medium unexpectedly stimulates the Aggrecan expression during the early osteogenic differentiation of GMSCs. This result of study is consistent with the research of Sumarta et al [14] which showed that Aggrecan expression increases significantly with PRF administration in culture medium. Furthermore, the triad tissue engineering consists of 3 elements, they are MSCs, natural scaffold and niche.…”

Section: Discussionsupporting

confidence: 93%

“…Growth factor contained in PRF plays an important role to enhance MSCs differentiation capability and acts as advantageous bio-scaffold. Biodegradable polymerized fibrin matrix forms networks that support and stimulate the beneficial MSCs secretome [5,6,14] .…”

Section: Discussionmentioning

confidence: 99%

“…Platelets Rich Fibrin is abundant with growth factor such as Insulin Growth Factor (IGF-I), Transforming Growth Factor-β1 (TGFβ-1), Vascular Endothelial Growth Factor (VEGF), Insulin Growth Factor (IGF-I), and Platelet Derived Growth Factor-β (PDGF-β) [6,14] . IGF stimulates aggrecan proteoglycan synthesis and suppresses catabolism of proteoglycans.…”

Section: Discussionmentioning

confidence: 99%

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Erken Osteojenik Farklılaşma (In Vitro) Süresince Wistar Sıçanlarda (Rattus novergicus) Gingival Medicinal Signaling Hücrelere Post Trombositten Zengin Fibrin Uygulamasının Agrekan Ekspresyonuna Etkisi

Nugraha¹,

Narmada²,

Ernawati³

et al. 2019

Kafkas Univ Vet Fak Derg

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Platelet Rich Fibrin (PRF) is rich for growth factors which can improve the Gingival Medicinal Signaling Cells' (GMSCs) osteogenic differentiation. Aggrecan is chondrogenic differentiation marker which has a significant role in the early stage of GMSCs' osteogenic differentiation. This study aimed to analyze the expression of Aggrecan post PRF administration on the osteogenic differentiation in vitro. This research is a true experimental study using the post-test only control group design with a simple random sampling. GMSCs were isolated from the lower gingival tissue of healthy male Wistar rats (Rattus novergicus) (n=4), weighted around 250 g, a month old, then cultured for 2 weeks and passaged for 4-5 days. GMSCs in the passage 3-5 were cultured in five M24 plates (N=54; n=6/group) for 7 days, 14 days, and 21 days in three different culture mediums, they were negative control group which included α Modified Eagle Medium; positive control group which contained High Glucose-Dulbecco's Modified Eagle Medium (DMEM-HG) combined with osteogenic medium; and at last, treatment group which were DMEM-HG combined with both osteogenic medium and PRF. A one-way Analysis of Variance (ANOVA) test (P<0.05) was performed. The treatment group showed the highest Aggrecan expression of 16.15±2.15 on the 7 th day. The lowest Aggrecan expression with a value of 3.67±0.76 on the 21 th day occurred in the negative control group. There was a significant difference of Aggrecan expression between groups (P<0.05). PRF administration unexpectedly stimulates Aggrecan expression of GMSCs during the osteogenic differentiation that useful to accelerate the bone remodeling or neo-cartilage formation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Erken Osteojenik Farklılaşma (In Vitro) Süresince Wistar Sıçanlarda (Rattus novergicus) Gingival Medicinal Signaling Hücrelere Post Trombositten Zengin Fibrin Uygulamasının Agrekan Ekspresyonuna Etkisi

Nugraha¹,

Narmada²,

Ernawati³

et al. 2019

Kafkas Univ Vet Fak Derg

Self Cite

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“…When the tube is removed from the centrifuge, three layers will appear that are divided into three sections; the lower section consists of red blood cells, the middle section contains PRF and the upper section is formed of acellular plasma. The PRF was then isolated after which the PRF was cut into small pieces using sterile scissors and inserted into each culture plate of the treatment group 22 , 28 , 29 .…”

Section: Methodsmentioning

confidence: 99%

Osteogenic potential of gingival stromal progenitor cells cultured in platelet rich fibrin is predicted by core-binding factor subunit-α1/Sox9 expression ratio (in vitro)

et al. 2018

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Background: Alveolar bone defect regeneration has long been problematic in the field of dentistry. Gingival stromal progenitor cells (GSPCs) offer a promising solution for alveolar bone regeneration. In order to optimally differentiate and proliferate progenitor cells, growth factors (GFs) are required. Platelet rich fibrin (PRF) has many GFs and can be easily manufactured. Core-binding factor subunit-α1 (CBF-α1) constitutes a well-known osteogenic differentiation transcription factor in SPCs. Sox9, as a chondrogenic transcription factor, interacts and inhibits CBF-α1, but its precise role in direct in vitro osteogenesis remains unknown. GSPCs cultured in vitro in PRF to optimally stimulate osteogenic differentiation has been largely overlooked. The aim of this study was to analyze GSPCs cultured in PRF osteogenic differentiation predicted by CBF-α1/Sox9. Methods: This study used a true experimental with post-test only control group design and random sampling. GPSCs isolated from the lower gingiva of four healthy, 250-gram, 1-month old, male Wistar rats ( Rattus Novergicus) were cultured for two weeks, passaged every 4-5 days. GSPCs in passage 3-5 were cultured in five M24 plates (N=108; n=6/group) for Day 7, Day 14, and Day 21 in three different mediums (control negative group: αModified Eagle Medium; control positive group: High Glucose-Dulbecco’s Modified Eagle Medium (DMEM-HG) + osteogenic medium; Treatment group: DMEM-HG + osteogenic medium + PRF). CBF-α1 and Sox9 were examined with ICC monoclonal antibody. A one-way ANOVA continued with Tukey HSD test (p<0.05) based on Kolmogorov–Smirnov and Levene's tests (p>0.05) was performed. Results: The treatment group showed the highest CBF-α1/Sox9 ratio (16.00±3.000/14.33±2.517) on Day 7, while the lowest CBF-α1/Sox9 ratio (3.33±1.528/3.67±1.155) occurred in the control negative group on Day 21, with significant difference between the groups (p<0.05). Conclusion: GSPCs cultured in PRF had potential osteogenic differentiation ability predicted by the CBF-α1/sox9 ratio.

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“…About 3-6 ml of blood was extracted with a 10ml syringe before being placed in a plain blood tube and swing centrifuged at 3,000 rpm/min for ten minutes) (Thermofisher, US). The PRF was then placed in a M24 culture plate of the treatment group [7,14].…”

Section: Platelet-rich Fibrin Extractionmentioning

confidence: 99%

<i>In vitro</i> bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

Nugraha¹,

Narmada²,

Ernawati³

et al. 2019

Trop. J. Pharm Res

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Purpose:To analyze the expression of bone sialoprotein -I (BSP -I) after the addition of platelet rich fibrin (PRF) in gingival somatic cell (GSC) culture medium during osteogenic differentiation in vitro. Methods: GSCs were extracted from healthy, 1-month-old, male Wistar rats (Rattus Novergicus), weighing 250 -300 g, and which had been randomly selected (n=4). These cells were cultured for 14 days and passaged every 4 days. Five subcultures of GSCs were cultured in three plates (M24) (N = 54; n = 6) for 7, 14 and 21 days in three preconditioned culture media (group I: plain culture media; group II: preconditioned osteogenic culture media, and group III: preconditioned osteogenic culture media with platelet rich fibrin). The expression of BSP-I was immunocytochemically (ICC) examined with monoclonal antibodies. Homogeneity and normality tests (p > 0.05) were then performed followed by an analysis of variance (ANOVA, p < 0.05). Results: The highest expression of BSP-I was found in group III (Day 21,13.00 ± 2.000), while the lowest expression of BSP-I was found in group I (Day 7, 7.33 ± 1.155). There were significant differences between the groups (p = 0.000, p < 0.05). Conclusion: PRF stimulates and significantly enhances the expression of BSP-I in GSC culture during osteogenic differentiation. Thus, PRF can be used to accelerate regeneration of alveolar bone defects. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study)

Cited by 6 publications

References 10 publications

Erken Osteojenik Farklılaşma (In Vitro) Süresince Wistar Sıçanlarda (Rattus novergicus) Gingival Medicinal Signaling Hücrelere Post Trombositten Zengin Fibrin Uygulamasının Agrekan Ekspresyonuna Etkisi

Erken Osteojenik Farklılaşma (In Vitro) Süresince Wistar Sıçanlarda (Rattus novergicus) Gingival Medicinal Signaling Hücrelere Post Trombositten Zengin Fibrin Uygulamasının Agrekan Ekspresyonuna Etkisi

Osteogenic potential of gingival stromal progenitor cells cultured in platelet rich fibrin is predicted by core-binding factor subunit-α1/Sox9 expression ratio (in vitro)

<i>In vitro</i> bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

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