Cholinesterases in Biorecognition and Biosensors Construction: A Review

Pohanka, Miroslav

doi:10.1080/00032719.2013.780240

Cited by 61 publications

(31 citation statements)

References 99 publications

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“…Especially, inhibitors of acetylcholinesterase and agonist on N-methy-D-aspartate receptors are available (33)(34)(35)(36)(37). Development of effective drugs is limited by fact that the etiology of Alzheimer disease remains unknown and the known therapies ameliorates manifestation only (38)(39)(40).…”

Section: Discussionmentioning

confidence: 99%

Caffeine alters oxidative homeostasis in the body of BALB/c mice

Pohanka¹

2014

BLL

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Caffeine is a metabolite from coffee genus and some other plants. It has disparate molecular targets in the body. Beside the major action based on antagonism on adenosine receptor, it is involved in other neurotransmission pathways as well. The complete mechanism of caffeine action remains unrevealed including link to oxidative homeostasis. For the reason, the current paper solves how caffeine infl uences oxidative homeostasis in BALB/c mouse model. Caffeine was given intramuscularly in doses 1-64 mg/kg and the animals were sacrifi ced in time intervals from 4 hours up to 3 days. In the experiment, a signifi cant increase of thiobarbituric acid reactive substances (TBARS) levels in the kidney and decrease in the brain indicated up and down regulation of oxidative burden. Ferric reducing antioxidant power (FRAP) level in the kidney appoint at release of low molecular weight antioxidants in the organ. The remaining organs including heart, muscles and liver were intact. In the same way, caspase 3 level was steady and no apoptotic processes were proved (Fig. 3

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Section: Discussionmentioning

confidence: 99%

Caffeine alters oxidative homeostasis in the body of BALB/c mice

Pohanka¹

2014

BLL

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“…Today acetylcholinesterase (AChE) is the most frequently used enzyme, due to its sensitivity to a number of toxic substances-pesticides, glycoalkaloids, etc. [2].…”

Section: Introductionmentioning

confidence: 95%

Feasibility of application of conductometric biosensor based on acetylcholinesterase for the inhibitory analysis of toxic compounds of different nature

Степурська

Солдаткін

Кучеренко

et al. 2015

Analytica Chimica Acta

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“…Enzymatic interaction is used for specific analyte recognition (Pohanka, 2013). The enzyme based biosensors provide specific advantages such as ability to catalyze several reactions, can detect many analytes such as substrates, products, modulators and inhibitors.…”

Section: Enzymatic Interactions Based Nanodiagnosticsmentioning

confidence: 99%

Nanodiagnostics: a new frontier for veterinary and medical sciences

Lambe¹,

Minakshi²,

Brar³

et al. 2016

JEBAS

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Infectious diseases are one of the greatest threats to animal and human population living in the developing world. These diseases have capacity to instigate in a small area and then open out very fast to the rest of the world and causing a heavy pandemic situation, for example; avian influenza pandemic. Such diseases infect large masses of population and may lead to loss of lives and also incur huge economic losses. Therefore, the best way to control these diseases is by diagnosing it at a very primary level and taking necessary precautionary measures so as to avoid the spread. Since last few years, the diagnostic approach has changed from tedious molecular biological techniques, to easy and rapid diagnostic techniques. Nanotechnology has extended the molecular diagnostics limit to nanoscale. These developed techniques do not require sophisticated laboratories and expert personnel, and hence are a cheap diagnostic approach. These assays can also be performed at the field level where the patient is present and get the results there itself. Hence, they are also called as pen side test or lab on chip diagnostic assays. The biological tests using nanotechnology become quicker, more flexible and more sensitive. These techniques have greatly influenced the diagnostic approach in the veterinary as well as medical field. Especially in the developing countries such as India, where the laboratory services are not

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Cholinesterases in Biorecognition and Biosensors Construction: A Review

Cited by 61 publications

References 99 publications

Caffeine alters oxidative homeostasis in the body of BALB/c mice

Caffeine alters oxidative homeostasis in the body of BALB/c mice

Feasibility of application of conductometric biosensor based on acetylcholinesterase for the inhibitory analysis of toxic compounds of different nature

Nanodiagnostics: a new frontier for veterinary and medical sciences

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