Cholinesterases

History and New Developments of Assays for Cholinesterase Activity and Inhibition

“…In the early development stage of spectrophotometric assays, a “remaining substrate” method based on the colorimetric determination of iron(III) hydroxamate was described. − Hydroxylamine reacts with the remaining acetylcholine and is converted to acetylhydroxamic acid. The latter forms a red complex with Fe 3+ that can be photometrically monitored in order to assay ChE activity.…”

Investigations on the Binding Sites of the Basement Membrane Zone for Pemphigoid Antibodies in Vitro. I. Enzymatic Action on the Active Groups of Human Skin

“…Thus, lysine and Fig. 4. Intensive basement mem brane zone fluorescence displayed by the indirect immunofluoreseence method, using cryostat seetions of human skin as antigenie substrate whieh had beeo preincubated for 5 minutes with lrypsin sol ution.…”

“…Cryostat sections of healthy human skin from different persons were incubated with enzymes of varying speci ficity (Table I) and after several thorough washings with Coons' buffer, were cxamined by the indirect immuno fluorescence method against experimental preparations which had not previously been incubated (normal series), with regard to the intensity of fluorescence and leve) of titre. For the indirect immunotluorescence procedure (2,4,10), the cryostat sections were incubated with a serum containing BMZ antibodies (using guinea pig tongue as an1igenic substrate. positive up to a dilution of I : 256).…”