Cholinergic activity modulates the survival of retinal ganglion cells in culture: the role of M<sub>1</sub> muscarinic receptors

Pereira, S.P.F.; Medina, Simone Vidal; Araújo, Elizabeth Giestal de

doi:10.1016/s0736-5748(01)00043-0

Cited by 23 publications

(20 citation statements)

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“…* P < 0.05 vs. the vehicle-treated group. (26) retinal ganglion cell cultures. However, neither mecamylamine nor scopolamine blocked the neuroprotective effects of donepezil on the retinal ganglion cells in the present study, suggesting that activation of nicotinic acetylcholine receptors or muscarinic acetylcholine receptors is not related to the neuroprotective effects of donepezil in the ganglion cells of the ischemic-reperfused rat retina.…”

Section: Dicussionmentioning

confidence: 99%

Histological Protection by Donepezil Against Neurodegeneration Induced by Ischemia–Reperfusion in the Rat Retina

et al. 2010

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Abstract. Although a blockade of acetylcholine esterase has been reported to suppress neuronal cell death induced by exogenous glutamate and β -amyloid, information is still limited regarding the neuroprotective effects of the acetylcholine esterase inhibitor donepezil. We histologically examined the effects of donepezil on neuronal injury induced by ischemia-reperfusion. Intravenous and intravitreous treatment with donepezil 15 min prior to ischemia dramatically reduced the retinal damage. The protective effect of donepezil in the ganglion cell layer was not affected by mecamylamine, a nicotinic acetylcholine-receptor antagonist, nor scopolamine, a muscarinic acetylcholine-receptor antagonist. The protective effect of donepezil in the inner plexiform layer was reduced not by mecamylamine, but by scopolamine. Neostigmine, a choline-esterase inhibitor, and pilocarpine, a muscarinic acetylcholine-receptor agonist, have protective effects in the inner plexiform layer and the inner nuclear layer. These results suggest that not only the activation of acetylcholine receptors but also a mechanism unrelated to acetylcholine-esterase inhibition contribute to the protective effect of donepezil on the ganglion cells in the ischemic-reperfused rat retina. Donepezil may be useful as a therapeutic drug against retinal diseases that cause neuronal cell death such as glaucoma with high intraocular pressure.

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Section: Dicussionmentioning

confidence: 99%

Histological Protection by Donepezil Against Neurodegeneration Induced by Ischemia–Reperfusion in the Rat Retina

et al. 2010

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“…Retinal cell cultures were prepared using procedures previously described [15] . P0-P2 rats were killed by decapitation, and neural retinas were dissected at 4 ° C. The tissue was incubated in 0.125% trypsin and 0.2% alidase (Sigma, Munich, Germany) for 30 min at 37 ° C. Activity of exogenous proteases was eliminated by washing the tissue with DMEM containing 10% FBS.…”

Section: Retinal Neuron Culturesmentioning

confidence: 99%

In vitro Study of the Effects of Lens Extract on Rat Retinal Neuron Survival and Neurite Outgrowth

Wang

Wang²,

Wang³

et al. 2009

Ophthalmic Res

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Background: Optic nerve regeneration has previously been achieved by injuring the lens, which results in the release of lentogenic factors. However, these lentogenic factors are still unknown. Objectives: To investigate what were the lentogenic factors by examining the effects of lens extract and macrophage-conditioned medium (MCM) on the survival and the neurite outgrowth of rat retinal neurons in vitro. Methods: Retinal neurons were cultured in 4 groups: (1) Dulbecco’s modified Eagle’s medium (DMEM), (2) DMEMcontaining lens extract, (3) DMEM containing macrophage-conditioned medium (MCM-D), (4) DMEM and medium from macrophages grown with lens extract (MCM-L). Neurite outgrowth and neuron survival time were observed. The density of retinal neurons with neurites and the longest neurites of the cells were measured on days 1, 3 and 5. Results: Retinal neurons survive for 12–14 days in DMEM containing lens extract. However, the cells only survive for 6 days in DMEM and only 7 days in DMEM containing MCM-L or MCM-D. The present results indicate that lens extract may directly promote survival of rat retinal neurons and neurite outgrowth in vitro. The MCM also promoted cell survival and neurite outgrowth but its effects were weaker than that of the lens extract. We postulate that lens extract exerts its effect by direct neurotrophic effects and/or indirectly by activating macrophagesin vitro.

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“…Fatores solúveis secretados tanto por glias e neurônios como neurotransmissores (Pereira & Araújo, 2001), hormônios e fatores de crescimento tem sido implicados na formação arquitetônica do cérebro (Gomes et al, 2001). Observamos também uma diminuição do crescimento de rede axodendrítica que poderia ter acelerado o processo degenerativo em nossas culturas experimentais.…”

Section: Resultsunclassified

“…As culturas de células ganglionares da retina de ratos Lister Hooded (sobre as quais foram colocadas as diluições do óleo essencial de B. trimera) foram feitas de acordo com o protocolo experimental descrito por Pereira & Araújo (2001). As células foram semeadas numa concentração de 6,5x10 5 células/mL diluídas em meio 199, sobre lamínulas pré-tratadas com poli-L-ornitina, estas foram dispostas no fundo de uma placa de Petri de 35 mm.…”

Section: Extração Do óLeo Essencialunclassified

Atividade de Baccharis trimera (Less.) DC., (Asteraceae) sobre cultura de células in vitro

Losqui¹,

Rozete²,

Almeida³

et al. 2009

Rev. bras. farmacogn.

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RESUMO: O uso indiscriminado de plantas medicinais vem da crença de que produtos naturais não causam mal à saúde, porém assim como medicamentos sintéticos, as plantas possuem grupos de compostos farmacologicamente ativos que atuam nos organismos vivos, sendo necessário o conhecimento destes para avaliação de suas potencialidades terapêuticas e tóxicas. Estudos sobre propriedades químicas de plantas medicinais, associando o extrato destas às atividades toxicofarmacológicas, são pouco encontrados. O presente ensaio objetivou analisar a ação do óleo essencial de B. trimera (Less.) DC., espécie pertencente à família Asteraceae e popularmente conhecida como carqueja, sobre cultura de células do sistema nervoso central. Foram usadas células de retina de ratos neonatos tratadas pelo óleo de carqueja nas concentrações 1:500 e 1:1000, por um período de incubação de 4, 24 e 48 horas. Nossos resultados demonstram que a carqueja possui ação inibitória na diferenciação de células neuronais in vitro quando tratadas cronicamente. Observamos também uma redução na sobrevida de células neuronais e diminuição da proliferação de células gliais. Essa redução da proliferação de células da glia poderia estar interferindo na sobrevivência e diferenciação neuronal. Maiores estudos são necessários para se compreender os mecanismos envolvidos nesse efeito.Unitermos: Carqueja, óleo essencial, cultura de células, Baccharis trimera.ABSTRACT: "Activity of Baccharis trimera (Less.) DC. Asteraceae on culture of retinal ganglion cells in vitro". The indiscriminate use of medicinal plants comes from the belief that natural products don't cause harm to health, but as well as synthetic drugs, plants have groups of pharmacologically active compounds that act in living organisms, with the necessary knowledge of these groups to evaluate the therapeutic and toxic potential. Studies of chemical properties of medicinal plants, involving the toxicfarmacologics activities of these extracts are hardly found. This study aimed to analyze the action of essential oil of B. trimera (Less.) DC., a species belonging to the Asteraceae family and popularly known as carqueja, on cultured cells of central nervous system. We used retinal cells from newborn rats treated by carqueja's oil, with concentrations 1:1000 and 1:500, for an incubation period of 4, 24 and 48 hours. Our results showed that carqueja has inhibitory action on the differentiation of neuronal cells in vitro when treated chronically. We also observed a reduction in survival of neuronal cells and decreased proliferation of glial cells. This reduction of the proliferation of glial cells could be interfering with the survival and neuronal differentiation. Further studies are needed to understand the mechanisms involved in this effect.

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Cholinergic activity modulates the survival of retinal ganglion cells in culture: the role of M₁ muscarinic receptors

Cited by 23 publications

References 50 publications

Histological Protection by Donepezil Against Neurodegeneration Induced by Ischemia–Reperfusion in the Rat Retina

Histological Protection by Donepezil Against Neurodegeneration Induced by Ischemia–Reperfusion in the Rat Retina

In vitro Study of the Effects of Lens Extract on Rat Retinal Neuron Survival and Neurite Outgrowth

Atividade de Baccharis trimera (Less.) DC., (Asteraceae) sobre cultura de células in vitro

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Cholinergic activity modulates the survival of retinal ganglion cells in culture: the role of M1 muscarinic receptors

Cited by 23 publications

References 50 publications

Histological Protection by Donepezil Against Neurodegeneration Induced by Ischemia–Reperfusion in the Rat Retina

Histological Protection by Donepezil Against Neurodegeneration Induced by Ischemia–Reperfusion in the Rat Retina

In vitro Study of the Effects of Lens Extract on Rat Retinal Neuron Survival and Neurite Outgrowth

Atividade de Baccharis trimera (Less.) DC., (Asteraceae) sobre cultura de células in vitro

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Cholinergic activity modulates the survival of retinal ganglion cells in culture: the role of M₁ muscarinic receptors