Choline acetyltransferase immunohistochemistry in brains of alzheimer's disease patients and controls

Nagai, Toshisaburo; McGeer, Patrick L.; Peng, Jun; McGeer, Edith G.; Dolman, C. E.

doi:10.1016/0304-3940(83)90264-1

Cited by 199 publications

(43 citation statements)

References 9 publications

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“…The form and distribution of the ChoAcTase-positive cell bodies and nerve fibers in the striatum were similar to those described in lower primates (15,16) and in humans (12,17,18).…”

Section: Discussionsupporting

confidence: 56%

Selective loss of cholinergic neurons in the ventral striatum of patients with Alzheimer disease.

Lehericy

Hirsch

Cervera

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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Cholinergic neurons were studied by immunohistochemistry with an antiserum against human choline acetyltransferase in the caudate nucleus, putamen, and ventral striatum (including the nucleus accumbens) of three patients with Alzheimer disease and three control subjects. Immunoreactive cell bodies were mapped and counted. In the ventral striatum of patients with Alzheimer disease, a 60% decrease in the number of cholinergic neurons was observed, whereas in the caudate nucleus and putamen values for control subjects and patients were similar. To determine whether all neurons in the ventral striatum were affected, neuropeptide Y-containing neurons were also immunostained, mapped, and counted. The number of these neurons was the same in control subjects and patients with Alzheimer disease, indicating that neuronal loss is not generalized in the ventral striatum and may be specific to the cholinergic population.

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“…The form and distribution of the ChoAcTase-positive cell bodies and nerve fibers in the striatum were similar to those described in lower primates (15,16) and in humans (12,17,18).…”

Section: Discussionsupporting

confidence: 56%

Selective loss of cholinergic neurons in the ventral striatum of patients with Alzheimer disease.

Lehericy

Hirsch

Cervera

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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“…Part of it lies dorsal to the anterior perforated sub stance [3,4,7,8,10]. It is about 2 mm thick and has medial-lateral and anterior-posterior (A-P) diameters of roughly 10 mm.…”

Section: Introductionmentioning

confidence: 99%

“…The basal nucleus of Meynert contains large cholinergic cells that innervate the cerebral cortex but not the hippocampus [1,3,4,7,8]. Loss [1,7,8] or atrophy [9] of these large cells is a conspicuous feature of senile dementia of the Alzheimer's type (SDAT).…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of the Basal Nucleus of Meynert in Senile Dementia of Alzheimer’s Type

Turnbull¹,

McGeer²,

Beattie³

et al. 1985

Stereotact Funct Neurosurg

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The basal nuclei of Meynert are the principal sources of cholinerigic innervation of the cerebral cortex. It has been hypothesized that the depressed cortical glucose metabolic activity in senile dementia of Alzheimer’s type (SDAT) may result primarily from diminished activity and loss of these cells. The present study was designed to test the hypothesis that electrical stimulation of the basal nuclei would bring about clinical improvement and increase cortical glucose metabolic activity in SDAT. An electrode was implanted in September 1984 into the left basal nucleus of a 74-year-old man with SDAT. Repetitive cycles of stimulation for 9 months since have had no definite effect clinically but a follow-up positron emission tomography scan shows that cortical glucose metabolic activity was preserved in the ipsilateral temporal and parietal lobes while it declined elsewhere in the cortex.

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“…Therefore, the neuronal cell size in the Ch4 may vary, probably owing to neurotrophic factors such as NGF. However, morphometric studies on the cholinergic neurons using immunocytochemical procedures have evaluated only neuronal cell counts (Nagai et al, 1983), and precise data on the morphometrical differences between cholinergic and noncholinergic neurons are not yet available. We studied the size of the nbM neurons using two sorts of preparations including cresyl violet and the indirect im- 195 munoperoxidase method with a monoclonal antiacetylcholinesterase (AChE) antibody counterstained with cresyl violet, focusing on both cholinergic and noncholinergic neurons.…”

Section: Introductionmentioning

confidence: 99%

Morphometric study on the CH4 of the nucleus basalis of meynert in Alzheimer’s disease

Kobayashi

Miyazu

Fukutani

et al. 1991

Molecular and Chemical Neuropathology

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Ch4 neurons were studied morphometrically in three normal controls and three patients with Alzheimer's disease (AD). Two series of preparations stained with cresyl violet and with the indirect immunoperoxidase method using a monoclonal antibody to acetylcholinesterase (AChE) counterstained with cresyl violet were morphometrically analyzed. The cholinergic neurons were identified as positive reaction products of AChE in the perikarya. The cross-sectional area of all the Ch4 neurons with clearly visible nucleoli in one preparation was measured using a computer imaging system. Evaluation of these data provided a mean value of the cross-sectional neuronal area (CNA), number of neurons, standard deviation of the CNA and coefficient of variation of the cholinergic and noncholinergic neurons. The cholinergic neurons were decreased in number but showed hypertrophy in the early stage of AD, and they gradually became atrophic in the advanced stage. The noncholinergic neurons showed only *Author to whom all correspondence and reprint requests should be addressed. atrophy without definite neuronal cell depletion. These findings indicate that cholinergic neurons have a plasticity and remodeling property against the AD process and suggest that hypertrophy of the cholinergic neurons in the Ch4 is the result of the effect of neurotrophic factors.

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Choline acetyltransferase immunohistochemistry in brains of alzheimer's disease patients and controls

Cited by 199 publications

References 9 publications

Selective loss of cholinergic neurons in the ventral striatum of patients with Alzheimer disease.

Selective loss of cholinergic neurons in the ventral striatum of patients with Alzheimer disease.

Stimulation of the Basal Nucleus of Meynert in Senile Dementia of Alzheimer’s Type

Morphometric study on the CH4 of the nucleus basalis of meynert in Alzheimer’s disease

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