Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic Lactobacilli and Bifidobacteria

Miremadi, Fatemeh; Ayyash, Mutamed; Sherkat, Frank; Stojanovska, Lily

doi:10.1016/j.jff.2014.05.002

Cited by 146 publications

(114 citation statements)

References 42 publications

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“…Cholesterol removal by probiotic Lactobacillus strains was determined according to the spectrophotometer method which previously described by Miremadi et al [14]. 30 mg of water-soluble cholesterol (poly-oxyethanyl-cholesterol sebacate) (Sigma, France) were dissolved in 10 ml of milli-q water and filter-sterilized to obtain a stock solution of cholesterol.…”

Section: Screening Of Probiotic Lactobacillus Strains For Cholesterolmentioning

confidence: 99%

“…The concentration of cholic acid was determined using spectrophotometer method as previously described by Miremadi et al [14]. 10 ml of MRS broth were supplemented with 6 mM of sodium glycocholate, 6 mM of sodium taurocholate, and a mixture of 2.8 mM of sodium glycocholate and 1.2 mM of sodium taurocholate [27].…”

Section: Deconjugation Of Bile Salts: Ammount Of Free Cholic Acidmentioning

confidence: 99%

“…Since then, the hypocholesterolamic effect of fermented dairy products has been investigated in human nutrition [13]. These studies suggest that Lactobacillus strains could eliminate total cholesterol and reduce density lipoprotein (LDL) cholesterol, which is a promoter facteur on serum cholesterol levels [14].…”

Section: Introductionmentioning

confidence: 99%

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Symbiotic, Hypocholesterolemic and Antioxidant Effects of Potential Probiotic Lactobacilli Strains Isolated from Tunisian Camel Milk

Mahmoudi¹,

Moussa²,

Hassouna³

2017

AiM

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In the present study, 20 selected Lactobacillus strains already characterized in a previous research for their capability to grow in conditions simulating the intestinal environment, their resistance to antibiotics, their antibacterial activity and their adhesion capability to intestinal human Caco-2 TC7 and HT-29 MTX cell lines, were further investigated to explore more their probiotic properties. Growth behaviour in the presence of prebiotic (fructooligosaccharides (FOS) and lactulose) at a concentration of 2%, cholesterol removal by measuring the residual cholesterol in medium supplemented with cholesterol, ability to deconjugate bile salts using BSH enzyme and antioxidant activity of culture supernatant of Lactobacillus strains by ABTS .+ and DPPH methods were analyzed. All probiotic strains demonstrated important prebiotic assimilation (P > 0.05) even with OD 600 > 3 after 30 h of contact, cholesterol removal ability with maximum percentage of 57% after 24 h of contact and they were found to liberate significantly (P < 0.05) more cholic acid with maximum of 0.40 mM of sodium glycocholate, 0.33 mM of sodium taurocholate and 0.41 mM of their mixte and scavenge both radicals with 52% and 2.19% of ABTS .+ and DPPH respectively. This study confirmed the suitability of these probiotic strains for application in functional food formulations especially where cholesterol reduction and antioxidant activity in food are needed to assess possible in vivo human health benefits.

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Section: Screening Of Probiotic Lactobacillus Strains For Cholesterolmentioning

confidence: 99%

Section: Deconjugation Of Bile Salts: Ammount Of Free Cholic Acidmentioning

confidence: 99%

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Symbiotic, Hypocholesterolemic and Antioxidant Effects of Potential Probiotic Lactobacilli Strains Isolated from Tunisian Camel Milk

Mahmoudi¹,

Moussa²,

Hassouna³

2017

AiM

View full text Add to dashboard Cite

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“…Other properties of probiotic organisms include, reduced deportment of pathogenic microorganism, lowered risk factors for coronary artery disease and a dose dependent reduction in the symptoms of irritable bowel syndrome (Vries et al, 2006). Several probiotics bacteria are found to produce bile salt hydrolase (BSH) that helps to reduce serum cholesterol (Miremadi et al, 2014) and hence BSH activity is also considered as an additional criterion for the selection of probiotics.…”

Section: Issn: 2319-7706 Volume 6 Number 3 (2017) Pp 1655-1675mentioning

confidence: 99%

Identification and Characterization of Bile Salt Hydrolyzing Lactobacillus Isolates

Sharma¹,

Kumar²,

Dhingra³

2017

Int.J.Curr.Microbiol.App.Sci

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“…Other functional properties used to characterize probiotics are the hypocholesterolemic and antimicrobial activities (5). Many researchers demonstrated the role of bile salts hydrolases (BSH) of probiotics in the reduction of serum cholesterol (6). Hence, the research of probiotics with BSH activities is also considered as an additional criterion for the selection of probiotics.…”

Section: Introductionmentioning

confidence: 99%

Bile salt hydrolase and antimicrobial activities of three bile resistant probiotics Lactobacillus plantarum strains isolated from Cameroonian artisanal fermented milk

Landry¹,

Pierre-Marie²,

Hector³

et al. 2017

J Microbiol Biotech Res

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Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic Lactobacilli and Bifidobacteria

Cited by 146 publications

References 42 publications

Symbiotic, Hypocholesterolemic and Antioxidant Effects of Potential Probiotic Lactobacilli Strains Isolated from Tunisian Camel Milk

Symbiotic, Hypocholesterolemic and Antioxidant Effects of Potential Probiotic Lactobacilli Strains Isolated from Tunisian Camel Milk

Identification and Characterization of Bile Salt Hydrolyzing Lactobacillus Isolates

Bile salt hydrolase and antimicrobial activities of three bile resistant probiotics Lactobacillus plantarum strains isolated from Cameroonian artisanal fermented milk

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