Symbiotic, Hypocholesterolemic and Antioxidant Effects of Potential Probiotic Lactobacilli Strains Isolated from Tunisian Camel Milk

Abstract

In the present study, 20 selected Lactobacillus strains already characterized in a previous research for their capability to grow in conditions simulating the intestinal environment, their resistance to antibiotics, their antibacterial activity and their adhesion capability to intestinal human Caco-2 TC7 and HT-29 MTX cell lines, were further investigated to explore more their probiotic properties. Growth behaviour in the presence of prebiotic (fructooligosaccharides (FOS) and lactulose) at a concentration of 2%, cholesterol removal by measuring the residual cholesterol in medium supplemented with cholesterol, ability to deconjugate bile salts using BSH enzyme and antioxidant activity of culture supernatant of Lactobacillus strains by ABTS .+ and DPPH methods were analyzed. All probiotic strains demonstrated important prebiotic assimilation (P > 0.05) even with OD 600 > 3 after 30 h of contact, cholesterol removal ability with maximum percentage of 57% after 24 h of contact and they were found to liberate significantly (P < 0.05) more cholic acid with maximum of 0.40 mM of sodium glycocholate, 0.33 mM of sodium taurocholate and 0.41 mM of their mixte and scavenge both radicals with 52% and 2.19% of ABTS .+ and DPPH respectively. This study confirmed the suitability of these probiotic strains for application in functional food formulations especially where cholesterol reduction and antioxidant activity in food are needed to assess possible in vivo human health benefits.

Levilactobacillus brevis from carnivores can ameliorate hypercholesterolemia: In vitro and in vivo mechanistic evidence

“…The biological effects of probiotic strains of lactic acid bacteria (LAB) are considered source, strain and species specific (Brunser & Gotteland, 2010). Variable hypocholesterolaemic potential of probiotic strains of LAB has also been linked with the use of different strains and sources (Mahmoudi et al, 2017; Moumita et al, 2018; Pereira et al, 2003). The strain specificity of probiotics intensified the research on different sources.…”

“…The strain specificity of probiotics intensified the research on different sources. Apart from food products, hypocholesterolaemic LAB can be isolated from the gastric tract of humans (Shin et al, 2010) and animals (Mahmoudi et al, 2017). Carnivorans, including wild and captive felids, are believed to tolerate high purines and fat‐based diets due to gut microbiome or genomic adaptations (Zhu et al, 2018) and may be exploited as a potent source of hypocholesterolaemic LAB strains.…”

Beneficial Effects of Probiotic Lactobacillus plantarum Isolated from Cow, Goat and Sheep Raw Milks

“…Cholesterol removal by probiotic Lactobacillus strains was determined 6, 12 and 24h of contact according to the spectrophotometer method which previously described by Miremadi., et al [11] and Mahmoudi., et al [12]. The ability of Lactobacillus plantarum to assimilate cholesterol was expressed as follows:…”

“…The supernatants were subsequently incubated respectively at 37°C and 45°C for 2h. The The antioxidant capacity was determined using ABTS .+ (2,2 azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical cation method which previously reported by Pieniz., et al [6] and Mahmoudi., et al [12]. The percentage inhibition of ATBS .+ was determined using ascorbic acid standard curve.…”

“…Mahmoudi., et al [12]. The results were determined using standard curve and expressed as EC 50 (µg/mL), which is the minimum concentration to decrease 50% of the initial DPPH reaction.…”