1995
DOI: 10.1111/j.1570-7458.1995.tb01898.x
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Cholesterol oxidase: an oöstatic and larvicidal agent active against the cotton boll weevil, Anthonomus grandis

Abstract: The enzyme cholesterol oxidase (E.C. 1.1.3.6), purified from Streptomyces culture filtrate was previously found to have oral insecticidal activity on neonate larvae of the boll weevil (Anthonomus grandis grandis Boheman) from a laboratory population. In the present study, second instar larvae were also controlled by the enzyme at diet concentrations similar to those which control neonates (12 day LC50 = 2.4 μg.ml−1 in diet). Larvae from field‐collected adults were similarly susceptible to cholesterol oxidase i… Show more

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Cited by 39 publications
(23 citation statements)
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“…This enzyme is industrially important and useful for the clinical determination of total or free serum cholesterol by coupling with related enzymes (Allain et al, 1974) and also for biological control of pests (Corbin et al, 1994(Corbin et al, , 2001. Moreover, cho as a secreted bacterial enzyme has lethal effect on the boll weevil (Anthonomus grandis grandis Boheman) and on several species of lepidopteran cotton insect pests including tobacco budworm (Heliothis virescens), corn earworm (Helicoverpa zea), and pink bollworm (Pectinophora gossypiella) (Perlak et al, 1990;Greenplate et al, 1995Greenplate et al, , 1997Sharma et al, 2000).…”
Section: Introductionmentioning
confidence: 99%
“…This enzyme is industrially important and useful for the clinical determination of total or free serum cholesterol by coupling with related enzymes (Allain et al, 1974) and also for biological control of pests (Corbin et al, 1994(Corbin et al, , 2001. Moreover, cho as a secreted bacterial enzyme has lethal effect on the boll weevil (Anthonomus grandis grandis Boheman) and on several species of lepidopteran cotton insect pests including tobacco budworm (Heliothis virescens), corn earworm (Helicoverpa zea), and pink bollworm (Pectinophora gossypiella) (Perlak et al, 1990;Greenplate et al, 1995Greenplate et al, , 1997Sharma et al, 2000).…”
Section: Introductionmentioning
confidence: 99%
“…Several research centers have made efforts to search and evaluate new insecticidal proteins suitable for boll weevil control in particular lectin and amylase and proteinase inhibitors; however, according to Greenplate et al (1995), these molecules retard growth and increase the time of development of the parasite, but seem to afford little acute toxicity.…”
Section: Introductionmentioning
confidence: 99%
“…In neonate and 2 nd instar larvae fed on diets containing this enzyme, the epithelial cell layer of the midgut was disrupted at low doses and complete lysed at high ones. Greenplate et al (1995) showed the effect of cholesterol oxidase on fertility and egg viability using artificial bolls treated with a 50 µg/mL of enzyme solution, and concluded that cholesterol oxidase might represent an alternative to the insecticides currently used for boll weevil control.…”
Section: Introductionmentioning
confidence: 99%
“…Upon ingestion, this protein causes developmental arrest and death of boll weevil larvae (Purcell et al, 1993), and a marked decrease in fecundity of female adult boll weevils (Greenplate et al, 1995). The enzyme also exhibits more moderate insecticidal effects when ingested by several species of lepidopteran cotton insect pests including tobacco budworm (Heliothis virescens), corn earworm (Helicoverpa zea), and pink bollworm (Pectinophora gossypiella; Greenplate et al, 1997).…”
mentioning
confidence: 99%