Cholesterol metabolism. 6. The fate of ingested cholesterol in the intact rat

Abstract

The ether-extractable acids were separated into solid and liquid acid fractions, converted into methyl esters and fractionally distilled. The light petroleum-insoluble compounds were separated from the fractions. Analyses of the acids are given (Table 2): these show that the increases after cholesterol feeding occur in the solid, liquid and light petroleum-insoluble fractions. 4. The methyl esters of the light petroleuminsoluble esters were chromatographed and gave a number of fractions, some of which are probably ketonic bile acids (Table 4). We are indebted to the Medical Research Council for a personal grant (to D.C.E.) and a grant for expenses.

Cholesterol metabolism. 5. The excretion of acids after cholesterol ingestion in rats

“…The subject is being further investigated. The increase in the excretion of volatile acids is discussed in more detail in the succeeding communication (Cook, Edwards, Riddell & Thomson, 1955).…”

“…Some of the neutral and acidic products of the faecal excretion after the ingestion of cholesterol have been described Edwards & Cook, 1955). The object of this communication is to put the results of the several experiments on a comparable basis and to give a picture of the metabolism of cholesterol in the entire animal.…”