Cholesterol Loaded Cyclodextrin Supplementation Enhances the Cholesterol-to-Phospholipid Ratio and Diminishes Oxidative Stress in Jack Spermatozoa During Cryopreservation

Kumar, Praveen; Mehta, Jitendra; Ravi, S. K.; Dedar, R. K.; Purohit, G. N.; Legha, Ram Avtar; Tripathi, Bhupendra Nath

doi:10.1016/j.jevs.2020.103237

Cited by 2 publications

(3 citation statements)

References 51 publications

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“…The results of the sperm quality analysis in the present study agree with those of other studies on Poitou jackasses performed in the Northern Hemisphere (9,(18)(19)(20)(21)(22)30). These studies were done under similar conditions of animal number and age (26 animals and 4-7 years of age), but with higher repetitions than in the present study (24-150 ejaculates vs. 14 ejaculates).…”

Section: Discussionsupporting

confidence: 91%

“…The lower normal morphology observed in our study (77.3 vs. >90%) could be due to the criterion used to detect structural abnormalities despite the fact that other authors used the same dye technique (9,18,19). The progressive motility reported in our study (63.6%) was higher than that observed by Trimeche et al (20,21) (39-56%), but lower than that of the other studies (9,18,19,30), which obtained progressive motility values over 75%. These dissimilarities may be related to differences in the extender composition (21), evaluation objectivity (9, 18), and CASA system configuration (9,20,30) used in each study.…”

Section: Discussioncontrasting

confidence: 76%

“…The progressive motility reported in our study (63.6%) was higher than that observed by Trimeche et al (20,21) (39-56%), but lower than that of the other studies (9,18,19,30), which obtained progressive motility values over 75%. These dissimilarities may be related to differences in the extender composition (21), evaluation objectivity (9, 18), and CASA system configuration (9,20,30) used in each study. In addition to these results, this study reported new insights into the kinetic characteristics of Poitou donkey sperm.…”

Section: Discussioncontrasting

confidence: 76%

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Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

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The Baudet du Poitou is a vanishing donkey breed recognized for engendering robust working mules. In Chile, only two pure breed Poitou males exist, which belong to the Chilean army and are used for mule production. We performed an extensive sperm and seminal analysis of these two jackasses aged 3 and 6 years and investigated the use of a simple hypometabolic extender for sperm cryopreservation. Computer-assisted sperm analysis showed high motility, velocity, and linearity in sperm movement. The seminal plasma analysis revealed that sodium and chloride were the main electrolytes, and globulins were the main metabolites. Active and variable enzymatic activity was observed. New information is reported about gamma-glutamyltransferase, aspartate aminotransferase, zinc, and magnesium concentrations in seminal plasma of Poitou donkeys. Ejaculates among jackasses showed some variability due to individual variability and different stages in sexual maturation according to age. The freezability index analysis based in viability, total motility and progressive motility with Botucrio extender (57.1 ± 11.0%; 56.6 ± 20.0%; and 22.6 ± 10.3%, respectively) were significantly higher (p < 0.05, p < 0.0001, and p < 0.0001, respectively) than with HM-0 extender (42,6 ± 11.4%; 14.9 ± 5.1%; and 1.0 ± 2.5%, respectively). We report new information on Poitou donkey semen and cryopreservation in the Southern Hemisphere that could be useful in donkey breeding and conservation programs to develop strategies that improve the effectiveness of population management of this breed.

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