Cholesterol concentration in seminal plasma as a predictive tool for quality semen evaluation

Ljubić, Blanka Beer; Aladrović, Jasna; Marenjak, Terezija Silvija; Laškaj, Renata; Majić-Balić, Ivanka; Milinković-Tur, Suzana

doi:10.1016/j.theriogenology.2009.07.009

Cited by 43 publications

(29 citation statements)

References 22 publications

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“…Based on this relationship, we tried to evaluate HDL‐C concentrations in boar seminal plasma, but the concentrations were lower than the assay detection limits. Similar obstacles were reported in men (Marsillach et al, ) and bulls (Beer‐Ljubić et al, ), suggesting that HDL‐C levels in seminal plasma are insignificant. Instead, we measured total cholesterol levels, which include HDL‐C, LDL‐C, and VLDL‐C (very‐low‐density lipoprotein cholesterol) (Birtcher and Ballantyne, ), to provide an approximation of the levels of HDL‐C present in our samples.…”

Section: Discussionsupporting

confidence: 82%

Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Barranco

Roca

Tvarijonaviciute

et al. 2014

Molecular Reproduction Devel

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Abbreviations: BTS, Beltsville thawing solution; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; LPO, peroxidation of membrane lipids; OS, oxidative stress; PBS, EDTA-free phosphate-buffered saline; PON, paraoxonase; ROS, reactive oxygen species; RT, room temperature; SP, seminal plasma. 3 ABSTRACTThis study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON-1, extracellular) and type 2 (PON-2, intracellular) in boar semen. To evaluate PON-1, an entire ejaculate from each of ten boars was collected and the seminal plasma (SP) harvested after double centrifugation (1,500g; 10 min). SP was analysed for concentration as well as enzymatic activity of PON-1, and for total cholesterol levels.

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Section: Discussionsupporting

confidence: 82%

Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Barranco

Roca

Tvarijonaviciute

et al. 2014

Molecular Reproduction Devel

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“…The main results obtained with the functional enrichment analysis of differentially expressed proteins in seminal plasma from subjects presenting low sperm DNA fragmentation were the hyper‐representation of phospholipase activity and lipoprotein particle remodelling and level regulation processes. Studies have shown that these properties, associated with sperm lipid catabolism, are important for sperm energy and capacitation‐associated membrane remodelling to reach its fertilization potential . The proteins involved in these processes, phospholipase A1, phospholipase A2 and lipoprotein lipase were found exclusively in this group, suggesting an involvement of these proteins and proprieties with normal sperm function and their possible association with sperm DNA fragmentation.…”

Section: Discussionmentioning

confidence: 90%

Sperm nuclear DNA fragmentation rate is associated with differential protein expression and enriched functions in human seminal plasma

Intasqui¹,

Camargo²,

Giudice³

et al. 2013

BJU International

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Objective• To analyse the proteomic profile of seminal plasma with the aim of identifying the proteins and post-genomic pathways associated with sperm DNA fragmentation. Materials and Methods• A cross-sectional study including 89 subjects from a human reproduction service was carried out.• All semen samples were assessed for sperm DNA fragmentation using a comet assay.• Results from 60 sperm were analysed using Komet 6.0.1 software and the 'Olive tail moment' variable was used to stratify these into low and high sperm DNA fragmentation groups.• Seminal plasma proteins from the two groups were pooled and used for proteomic analysis.• Quantitative data were used for functional enrichment studies. Results• Seventy-two proteins were identified or quantified in seminal plasma. Of these, nine were differentially expressed in the low group and 21 in the high group. Forty-two proteins were conserved between these groups.• Functional enrichment analysis indicated that sperm DNA fragmentation was related to functions such as lipoprotein particle remodelling and regulation, fatty acid binding and immune response.• Proteins found exclusively in the low group may be involved in correcting spermatogenesis and/or improving sperm function. Proteins in the high group were associated with increased innate immune response, sperm motility and/or maturation and inhibition of mitochondrial apoptosis. Conclusion• Protein expression and post-genomic pathways of seminal plasma differ according to the rate of sperm DNA integrity.

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“…Our work was the first study that demonstrated the highest level of TG and CHO in PF of dogs with prostatomegaly and suggested their quantification in canine seminal plasma as biomarkers of prostate diseases in dogs. CHO in the seminal plasma has been used as a biomarker of sperm quality (Beer‐Ljubić et al., ). In dogs, a lower concentration of CHO was observed in the seminal plasma of dogs with bad quality after thawing (Schäfer‐Somi & Palme, ).…”

Section: Discussionmentioning

confidence: 99%

Serum testosterone, sperm quality, cytological, physicochemical and biochemical characteristics of the prostatic fraction of dogs with prostatomegaly

Aquino-Cortez

Pinheiro

Silva

et al. 2017

Reprod Domestic Animals

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Prostatomegaly is a common finding in older non-neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement. Sperm motility, vigour, concentration, viability, membrane functionality and morphology were analysed in sperm-rich fraction. Osmolality, pH, cell types, and albumin, haemoglobin, acid phosphatase, alkaline phosphatase, glucose, triglycerides, cholesterol, calcium, phosphorus, magnesium and chloride were analysed in prostatic fraction. Dogs with prostatomegaly have the lowest sperm motility, vigour, concentration and functional membrane. Dogs with prostatomegaly have the highest glucose, triglycerides and cholesterol. Glucose was the only constituent positively correlated with serum testosterone and prostate volume. It can be concluded that dogs with prostatomegaly have poorer sperm quality, and glucose, triglycerides and cholesterol in prostatic fraction can be used as prostatomegaly biomarkers.

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Cholesterol concentration in seminal plasma as a predictive tool for quality semen evaluation

Cited by 43 publications

References 22 publications

Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Sperm nuclear DNA fragmentation rate is associated with differential protein expression and enriched functions in human seminal plasma

Serum testosterone, sperm quality, cytological, physicochemical and biochemical characteristics of the prostatic fraction of dogs with prostatomegaly

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