Cholesterol biosensors prepared by layer-by-layer technique

Ram, Manoj K.; Bertoncello, Paolo; Ding, Hanming; Paddeu, Sergio; Nicolini, Claudio

doi:10.1016/s0956-5663(01)00208-1

Cited by 160 publications

(93 citation statements)

References 36 publications

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“…This indicates that ChOx still remains bioactive when it is immobilized on the MWNTs-modified gold electrode by LBL technique. Additionally, a response time of about 30 s can be observed from Figure 5, which is similar to the reported value for cholesterol biosensor (30 -40 s) [23]. …”

Section: Current Response Of the Enzyme Electrodesupporting

confidence: 86%

“…With the isoelectric point lying around 5.0, cholesterol oxidase (ChOx) can be used as polyanion at the desired pH (about 7.0), where it probably shows the maximum enzymatic activity. The deposition of ChOx on several substrates by LBL technique has been investigated [23]. According to our knowledge, LBL technique has never been used for the immobilization of enzyme on carbon nanotubes.…”

Section: Introductionmentioning

confidence: 99%

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Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

et al. 2004

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A carbon nanotubes-based amperometric cholesterol biosensor has been fabricated through layer-by-layer (LBL) deposition of a cationic polyelectrolyte (PDDA, poly(diallyldimethylammonium chloride)) and cholesterol oxidase (ChOx) on multi-walled carbon nanotubes (MWNTs)-modified gold electrode, followed by electrochemical generation of a nonconducting poly(o-phenylenediamine) (PPD) film as the protective coating. Electrochemical impedance measurements have shown that PDDA/ChOx multilayer film could be formed uniformly on MWNTsmodified gold electrode. Due to the strong electrocatalytic properties of MWNTs toward H 2 O 2 and the low permeability of PPD film for electroacitve species, such as ascorbic acid, uric acid and acetaminophen, the biosensor has shown high sensitivity and good anti-interferent ability in the detection of cholesterol. The effect of the pH value of the detection solution on the response of the biosensor was also investigated. A linear range up to 6.0 mM has been observed for the biosensor with a detection limit of 0.2 mM. The apparent Michaelis-Menten constant and the maximum response current density were calculated to be 7.17 mM and 7.32 mA cm À2, respectively.

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Section: Current Response Of the Enzyme Electrodesupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

et al. 2004

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“…Other examples for small molecule detection, in particular environmental contaminants include petroleum hydrocarbons (Fahnrich et al, 2002;Applebee et al, 2004), Cr 6þ , Cr 3þ , Cd 3þ , Cu 2þ and other ions (Howie and Hoagland, 2002;Mirmohseni and Oladegaragoze, 2002;Tani and Umezawa, 2003;Sung and Shih, 2005), estrogenic compounds (Murata et al, 2003;Luck et al, 2004), aliphatic amines (Mirmohseni and Oladegaragoze, 2003), phenol (Mirmohseni and Oladegaragoze, 2004), sulfur dioxide (Palenzuela et al, 2005), NO x (Seh et al, 2005), enantiomers , pesticides , doxorubicin (Serpe et al, 2005), cholesterol (Ram et al, 2001), limonene (Fietzek et al, 2001), alcohols (Loergen et al, 2005), dioxins Kurosawa et al, 2003) and cisplatinum drugs .…”

Section: Direct Receptor-small Molecule Interactionmentioning

confidence: 99%

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Cooper¹,

Singleton²

2007

J of Molecular Recognition

333

204

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The widespread exploitation of biosensors in the analysis of molecular recognition has its origins in the mid-1990s following the release of commercial systems based on surface plasmon resonance (SPR). More recently, platforms based on piezoelectric acoustic sensors (principally 'bulk acoustic wave' (BAW), 'thickness shear mode' (TSM) sensors or 'quartz crystal microbalances' (QCM)), have been released that are driving the publication of a large number of papers analysing binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights salient theoretical and practical aspects of the technologies that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and lipidic and polymeric interfaces. Key differentiators between optical and acoustic sensing modalities are also reviewed.

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“…It has been proved that LBL method is a promising way especially for the immobilization of biomolecules [15][16][17][18][19]. LBL method, which is based on electrostatic being forced between oppositely charged polyelectrolytes has been utilized on the immobilization of enzymes and construction of a biosensing system [20,21]. Various kinds of enzymes have been immobilized by LBL, and enzymes encapsulated in these integrated multilayer films showed superior chemical stability in long storage period and relative thermostability [22,23].…”

Section: Introductionmentioning

confidence: 99%

_L-Proline sensor based on layer-by-layer immobilization of thermostable dye-linked L-proline dehydrogenase and polymerized mediator

Zheng

Hirose

Kimura

et al. 2006

Science and Technology of Advanced Materials

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L-Proline sensor has been fabricated through multilayer assembly of recombinant dye-linked L-proline dehydrogenase (L-proDH) originally from hyperthermophilic archaeon (Therococcus profundus) and polymerized mediator, poly(allylamine) ferrocene (PAA-Fc), on a gold electrode by electrostatic layer-by-layer alternate adsorption method. The characteristic redox peaks of ferrocene groups were obvious exhibited in cyclic voltammograms, and both anodic and cathodic peaks increased with PAA-Fc/L-proDH bilayer number which confirmed the formation of a multilayer structure. Further experiments showed that an electrode fabricated in this way catalyzed the oxidation of L-proline. It suggested that polymerized mediator could transfer electrons between electrode surface and immobilized L-proDH. Ampeormetric experiments showed that the current response to L-proline increased with PAA-Fc/L-proDH bilayer number. The stability of the sensor was measured, and it kept a 40% relative response after 30 days storage in buffer under 4 8C. q

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Cholesterol biosensors prepared by layer-by-layer technique

Cited by 160 publications

References 36 publications

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

_L-Proline sensor based on layer-by-layer immobilization of thermostable dye-linked L-proline dehydrogenase and polymerized mediator

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