Cholesterol amperometric biosensor based on cytochrome P450scc

Shumyantseva, Victoria V.; Deluca, Gilda; Булко, Т. В.; Carrara, Sandro; Nicolini, Claudio; Усанов, С. А.; Archakov, Alexander I.

doi:10.1016/j.bios.2003.09.001

Cited by 88 publications

(64 citation statements)

References 19 publications

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“…Accordingly with our previous works [3,6,7], the screen-printed electrodes for P450 investigations were produced on flexible polyvinylchloride sheets from SKK (Denzlingen, Germany) using a DEK 249 screen printer (DEK Ltd., Weymouth, England) by using polyester screens from Steinmann GmbH (Stuttgart, Germany). Screen printing inks (Electrodag PF-410, Electrodag 6037 SS) were obtained from Acheson (Scheemda, Netherlands).…”

Section: Chemicalsmentioning

confidence: 99%

“…Similarly, different oxidases may be used to detect lactate, glutamate, cholesterol and other metabolic molecules which may be as relevant as diseases biomarkers [2]. The cholesterol may be also detected by using cytochromes P450 [3]. This is a protein family with more then 3000 different isoforms which may catalyze many different endogenous and exogenous compounds highly relevant for the human metabolism.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantum Dots and Wires to Improve Enzymes-Based Electrochemical Bio-sensing

Carrara

Boero

Micheli

2009

Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering

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Abstract. An investigation on nano-structured electrodes to detect different metabolites is proposed in this paper. Three different metabolites are considered: glucose, lactate, and cholesterol. The direct detection of hydrogen peroxide is also considered since it does not involve any enzyme. The metabolites and the peroxide were detected by using screen-printed electrodes modified by using multi-walled carbon nanotubes. In all cases, improvements of orders of magnitude were registered both on detection sensitivity and on detection limit. A close comparison with data recently published in literature has shown the existence of an inverse linear correlation between detection sensitivity and detection limit when sensor performances improve due to nanostructured materials. This inverse linear relationship seems to be a general law as it is here demonstrated for all the considered detections on glucose, lactate, chlesterol, and hydrogen peroxide.

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Section: Chemicalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantum Dots and Wires to Improve Enzymes-Based Electrochemical Bio-sensing

Carrara

Boero

Micheli

2009

Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering

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“…It has been shown in 2005 that P450 11A1 activity is highly enhanced when the cytochrome is immobilized onto screen-printed electrodes modified by gold nanoparticles [5]. 100 µM of cholesterol produced a current signal of a few µA when using riboflavin as molecular mediator between P450 11A1 and rhodium-graphite screenprinted electrodes with an area of 20 mm 2 [6]. On the other hand, a 10 µM concentration produced a current signal of more than 1 µA when using screen-printed electrodes of the same area but modified by gold nanoparticles [5].…”

Section: Introductionmentioning

confidence: 99%

Circuits design and nano-structured electrodes for drugs monitoring in personalized therapy

Carrara

Cavallini

Micheli

et al. 2008

2008 IEEE Biomedical Circuits and Systems Conference

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Abstract-Drug development and personalized therapy require accurate and frequent monitoring of the metabolic response by living tissues to treatments. In case of high risk side effects, e.g. therapies with interfering anti-cancer molecule cocktails, direct monitoring of the patient's drugs metabolism is essential as the metabolic pathways efficacy is highly variable on a patient-bypatient basis. Currently, there are no fully mature point-of-care bio-sensing systems for drugs metabolism monitoring directly in blood. The aim of the paper is to investigate solutions to develop point-of-care systems for drugs monitoring in personalized therapy. P450 enzymes are the considered probe molecules as they are key proteins directly involved in drugs metabolism of humans. Sensitivity improvement is ensured by means of enzyme integration onto electrodes structured with carbon nanotubes. Component Off-The-Shelf (COTS) based circuits design is investigated toward bio-chip development. Results show that the proposed circuitry is suitable for the aim and confirm that nanotubes are detection enhancers.

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“…Numerous methods based on enzyme immobilization for human cholesterol assays have been developed, including colorimetric, spectrometric and electrochemical methods (Crumbliss et al, 1993;Shumyantseva et al, 2004). The use of enzymes in the fabrication of sensors has advantages due to their rapid, selective, and sensitive nature.…”

Section: Enzyme-free Biosensor Based On Chemical Reactionmentioning

confidence: 99%