Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation

Abstract

To study the early pathogenesis of acute edematous pancreatitis in dogs, we examined the relationship of pancreatic hyperstimulation with cholecystokinin-8 (10 micrograms/kg/hr intravenously for 6 hr) to alterations in circulating pancreatic enzymes and pancreatic morphology with special reference to trypsinogen activation. Cholecystokinin-8 infusion was associated with increases in plasma amylase, lipase, trypsin-like immunoreactivity, and plasma and urine trypsinogen activation peptide. Pancreatic parenchymal swelling and interlobular and subcapsular fluid accumulations were detected ultrasonographically within 2 hr of cholecystokinin-8. Circulating trypsin-like immunoreactivity and trypsinogen activation peptide in urine reached a peak at 2 and 4 hr, respectively, then declined despite progressive increases in circulating amylase and lipase and intrapancreatic fluid. No significant changes were observed in dogs receiving a saline infusion. This study illustrates that cholecystokinin-8 induces edematous pancreatitis in dogs that is associated with a short-lived burst of trypsinogen activation.

Pancreatic Edema in Dogs with Hypoalbuminemia or Portal Hypertension

“…[1][2][3][4][5][6] Caerulein induces distortion of pancreatic capillary architecture and irregularity of the capillary endothelial lining, which seems to lead to edema as a result of altered capillary permeability. [4][5][6] Pancreatitis cannot be excluded as a contributing factor for pancreatic edema in most dogs this series because only 2 dogs had histologic examination of the pancreas; however, pancreatic edema probably occurred in these dogs as a result of the same factors that resulted in peritoneal effusion, namely hypoalbuminemia and portal hypertension.…”

“…[1][2][3][4][5][6] Edema fluid in the interlobular septa of the pancreas appears ultrasonographically as multiple linear anechoic areas that divide the gland, resulting in a distinctive ''tiger-stripe'' appearance. 1,3 The purpose of this report is to describe ultrasonographic signs of pancreatic edema in a series of dogs with naturally occurring diseases and to identify possible risk factors.…”

One-Step Immunochromatography Assay for Detection of High-Level Canine Serum Trypsin-Like Immunoreactivity

“…Serum cTLI levels determined by ELISA and RIA are significantly lower in dogs with exocrine pancreatic insufficiency (EPI) than in normal dogs [17,18]. We previously developed a simple bedside test using an immunochromatography system to determine low levels of cTLI in canine sera for diagnosis of EPI [15].On the other hand cTLI levels are apparently high in the early phase of experimentally induced pancreatitis [2,3,10,11,18]. Therefore, a simple method to assay cTLI levels at bedside will be helpful for evaluate not only EPI but also pancreatitis.…”

“…On the other hand cTLI levels are apparently high in the early phase of experimentally induced pancreatitis [2,3,10,11,18]. Therefore, a simple method to assay cTLI levels at bedside will be helpful for evaluate not only EPI but also pancreatitis.…”

“…Several papers demonstrated that cTLI levels are apparently high in the early phase of experimentally induced pancreatitis [2,3,10,11,18]. It was reported that the very low cTLI values observed in chronic pancreatitis due to the inadequate pancreatic function resulting from calcification of pancreatic tissues [16].…”