Cholecystokinin-58 is the major molecular form in man, dog and cat but not in pig, beef and rat intestine

Eberlein, Gert; Eysselein, Viktor E.; Goebell, H.

doi:10.1016/0196-9781(88)90079-4

Cited by 36 publications

(29 citation statements)

References 21 publications

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“…The enzymes causing this degradation could come from processing enzymes in the I cell (the cell that synthesizes intestinal cholecystokinin) or from pancreatic enzymes released into the intestinal lumen. However, we have shown a good relationship between the intestinal and blood forms of cholecystokinin in humans (2, 6) and dogs (1,5,16). Both tissues contain mainly CCK-58.…”

Section: Discussionmentioning

confidence: 99%

CCK-58 is the only detectable endocrine form of cholecystokinin in rat

J¹,

Green²,

Chew³

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

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CCK-58 differs from CCK-8 in patterns of expression of pancreatic secretion of fluid and amylase and gallbladder contraction. These differences have physiological relevance only if CCK-58 release is stimulated by nutrients entering the intestine and if CCK-58 circulates in sizeable amounts. In this study, we report that when radiolabeled CCK-58 is added to rat blood and plasma is formed, there is extensive loss and degradation of the radioactive peptide. Therefore, a new method was developed to minimize loss and degradation of this label. This method recovered >85% of the label with no detectable degradation. Furthermore, the optimized method recovered all unlabeled exogenous cholecystokinin molecular forms in >80% yields. Blood from fasted rats and rats in which cholecystokinin release was stimulated by the trypsin inhibitor camostat contained only CCK-58 (3.5 ± 0.5 and 17 ± 1.5 fmol/ml, respectively). Because CCK-58 predominates in the blood, this molecular form should be used in studies on the physiology and pathophysiology of cholecystokinin.

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Section: Discussionmentioning

confidence: 99%

CCK-58 is the only detectable endocrine form of cholecystokinin in rat

J¹,

Green²,

Chew³

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…The complexity of the differential processing of pro-CCK to various bioactive products is reflected by the large number of amidated forms that have been isolated from different tissues and species including CCK 8 (44,45), CCK 22 (46), CCK 33 (47), CCK 58 (48), and CCK 83 (48). Several different processing pathways have been proposed based on these CCK intermediates (49,50), which suggests a regulatory mechanism for the fate of pro-CCK during processing.…”

Section: Discussionmentioning

confidence: 99%

Prohormone Convertase 1 Is Necessary for the Formation of Cholecystokinin 8 in Rin5F and STC-1 Cells

Yoon

Beinfeld

1997

Journal of Biological Chemistry

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“…Thus, endogenous endocrine CCK-8 detected by others (23,35) is most likely a product of peptide degradation during plasma formation. Importantly, CCK-58 is the major intestinal and endocrine form in dogs (8,13), rats (42), and humans (8,9,14), suggesting its biological action should be evaluated and the influence of CCK-58 on feeding patterns should be studied. The importance of this suggestion is indicated by the fact that CCK-8 and CCK-58 show different actions on various physiological processes.…”

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confidence: 99%