Choice of Time for Urine Collection for Detecting Early Kidney Abnormalities in Hypertensives

Abstract

SUMMARY.The aim of this study was to determine the most appropriate urine collection for detecting differences in the excretion rates of albumin, -y glutamyl trans peptidase (GGT) and N-acetyl-(j-D-glucosaminidase (NAGA) between normotensive subjects and hypertensive patients on treatment. Twenty treated hypertensive patients, mean (SEM, standard error of mean) age; 52·2 (6'2) years and 20 normotensive subjects, mean age 49· 2 (4'2) years, were studied in a consecutive sampling design. Urinary excretion rates of albumin, GGT and NAGA were determined in consecutive timed urine samples collected overnight and during 3-5 h the next morning. Mean (SEM) overnight excretion rates for albumin, GGT and NAGA for normotensive subjects were 11·05 (1'18) ltg/min, 17·00 (2'20)mU/min and 6·55 (0'39)mU/min, respectively, which were significantly lower than those of hypertensive subjects which were 20· 77 (2' 14) ltg/min, 21· 84 (1'65) mU/min and 10·92 (0'87) mU/min, respectively (P<0·05). The mean (SEM) percentage increases in urinary albumin, GGT and NAGA in morning urine collections of normotensive subjects of 15· 22 (3' 88)0/0, 34' 04 (6' 45)% and 11· 54 (3' 63)%, respectively were significantly lower than 107·03 (15'04)%,121'96 (16'71)% and 72· 75 (7' 50)% found in hypertensive patients (P< 0,05). These data suggest that were urinary albumin and tubular enzyme excretion to be used as correlates of hypertensive renal damage, ambulatory urine collections may be more sensitive than overnight collections.

Preliminary Study for the Effect of Tramadol Addiction on Functional and Structural Integrity of Renal Proximal Tubules Among Addicted Egyptians

“…Morning urine sample was suggested as the best sample for detecting early kidney abnormalities (Zuppi, et al, 1995). Spot morning urine sample was collected by each participant who was instructed to void the urine sample directly into 100 ml sterilized plastic container and centrifuged at 4500 rpm for 5 minutes, and then the clear supernatant was distributed in polyethylene vials (1.5 ml capacity).…”

Effect of mercury vapour exposure on urinary excretion of calcium, zinc and copper: relationship to alterations in functional and structural integrity of the kidney

“…Morning urine was suggested as the best sample for detecting early kidney abnormalities (Zuppi et al, 1995). Spot morning urine sample was collected by each participant who was instructed to void the urine sample directly into 100 mL metal-free polyethylene bottle.…”

The influence of urinary flow rate in children on excretion of markers used for assessment of renal damage: albumin, γ-glutamyl transpeptidase, N-acetyl-β-D-glucosaminidase, and alpha1-microglobulin

“…Many additional studies have also found that γ-GT [16,17], albumin [16][17][18][19][20], and NAG [18,21,22] excretion rates are significantly higher during the day than overnight. Some studies cite urinary flow rate as a possible cause for this finding [20,21,23].…”