Choice of Oxygen-Conserving Treatment Regimen Determines the Inflammatory Response and Outcome of Photodynamic Therapy of Tumors

Henderson, Barbara W.; Gollnick, Sandra O.; Snyder, John W.; Busch, Theresa M.; Kousis, Philaretos C.; Cheney, Richard; Morgan, Janet

doi:10.1158/0008-5472.can-03-3513

Cited by 232 publications

(270 citation statements)

References 27 publications

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“…With decreasing oxygen concentration, the quantum yield of singlet oxygen is also decreasing (42). Therefore, the light excitation of photosensitizers in photodynamic therapy of cancer is fractionated by using irradiation breaks of several minutes to allow oxygen supply to tumor areas (24,(43)(44)(45). This procedure was also used with biofilms of Streptococcus mutans, which were efficiently inactivated by light dose fractionation (46).…”

Section: Singlet Oxygen Luminescence For Variable and High Bacterial mentioning

confidence: 99%

“…This time-resolved decay of the luminescence should provide an indication of the site of singlet oxygen in bacteria. In addition, the generation of singlet oxygen is very sensitive to oxygen concentration, which may play a role in the photodynamic inactivation of bacteria comparable to that in eukaryotic cells (24). The experiments were carried out with Photofrin, one of the most widely used and clinically approved photosensitizers in photodynamic therapy, which also demonstrates antimicrobial efficacy (25)(26)(27).…”

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confidence: 99%

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The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria

Maisch¹,

Baier²,

Franz³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

363

272

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New antibacterial strategies are required in view of the increasing resistance of bacteria to antibiotics. One promising technique involves the photodynamic inactivation of bacteria. Upon exposure to light, a photosensitizer in bacteria can generate singlet oxygen, which oxidizes proteins or lipids, leading to bacteria death. To elucidate the oxidative processes that occur during killing of bacteria, Staphylococcus aureus was incubated with a standard photosensitizer, and the generation and decay of singlet oxygen was detected directly by its luminescence at 1,270 nm. At low bacterial concentrations, the time-resolved luminescence of singlet oxygen showed a decay time of 6 ؎ 2 s, which is an intermediate time for singlet oxygen decay in phospholipids of membranes (14 ؎ 2 s) and in the surrounding water (3.5 ؎ 0.5 s). Obviously, at low bacterial concentrations, singlet oxygen had sufficient access to water outside of S. aureus by diffusion. Thus, singlet oxygen seems to be generated in the outer cell wall areas or in adjacent cytoplasmic membranes of S. aureus. In addition, the detection of singlet oxygen luminescence can be used as a sensor of intracellular oxygen concentration. When singlet oxygen luminescence was measured at higher bacterial concentrations, the decay time increased significantly, up to Ϸ40 s, because of oxygen depletion at these concentrations. This observation is an important indicator that oxygen supply is a crucial factor in the efficacy of photodynamic inactivation of bacteria, and will be of particular significance should this approach be used against multiresistant bacteria.luminescence ͉ oxygen depletion ͉ Staphylococcus aureus

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Section: Singlet Oxygen Luminescence For Variable and High Bacterial mentioning

confidence: 99%

mentioning

confidence: 99%

The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria

Maisch¹,

Baier²,

Franz³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

363

272

View full text Add to dashboard Cite

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“…For example, one indirect method is the use of light fractionation in which delivery of the red light is interrupted at a particular point for a short period of time [14]. It has been suggested that in the absence of light blood flow is restored [7] and using a lower fluence rate oxygen consumption is slowed [33,34] both allowing for tissue re-oxygenation [33,35]. However, it is widely accepted that the tumor environment is more hypoxic than the surrounding normal tissue, suggesting pre-existing hypoxic cells would be unaffected by reducing fluence rate or light fractionation [36] and thus more direct approaches of oxygenation have also been investigated.…”

Section: Discussionmentioning

confidence: 99%

Effect of an oxygen pressure injection (OPI) device on the oxygen saturation of patients during dermatological methyl aminolevulinate photodynamic therapy

et al. 2012

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Methyl aminolevulinate photodynamic therapy (MAL-PDT) (a topical treatment used for a number of pre-cancerous skin conditions) utilizes the combined interaction of a photosensitizer (protoporphyrin IX (PpIX)), light of the appropriate wavelength and molecular oxygen to produce singlet oxygen and other reactive oxygen species which induce cell death. During treatment localized oxygen depletion occurs and is thought to contribute to decreased efficacy.The aim of this study was to investigate whether an oxygen pressure injection (OPI) device had an effect on localized oxygen saturation levels and/or PpIX fluorescence of skin lesions during MAL-PDT. This study employed an OPI device to apply oxygen under pressure to the skin lesions of patients undergoing standard MAL-PDT. Optical reflectance spectrometry and fluorescence imaging were used to non-invasively monitor the localized oxygen saturation and PpIX fluorescence of the treatment area respectively. No significant changes in oxygen saturation were observed when these data were combined for the group with OPI and compared to the group that received standard MAL-PDT without OPI. Additionally no significant difference in PpIX photobleaching or clinical outcome at three months between the groups of patients was observed although the group that received standard MAL-PDT demonstrated a significant increase (p<0.05) in PpIX fluorescence initially and both groups produced a significant decrease (p<0.05) after light irradiation. In conclusion, with this sample size this OPI device was not found to be an effective method with which to improve tissue oxygenation during MAL-PDT. Further investigation is therefore required to find a more effective method of MAL-PDT enhancement.3

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“…Several factors such as PS dose, the light energy given, and the interval time between PS injection and light irradiation, are known to affect the outcome of PDT. 17,32,33) Since PDT with a high PS dose was revealed to cause severe damage to tumor vessels, 34) the treatment conditions have to be minutely determined to extract the desired action on the tumor vasculature. In addition, since the lifetime of singlet oxygen produced by light irradiation to PS is very short (dozens of nanoseconds), the area that the molecule can reach within its lifetime is small.…”

Section: Application Of Photodynamic Treatment To Enhance Tumor Vascumentioning

confidence: 99%

Nanoparticle-Based Photodynamic Therapy: Current Status and Future Application to Improve Outcomes of Cancer Treatment

Ogawara

Higaki

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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Photodynamic therapy (PDT) is an emerging cancer treatment that uses photosensitizers (PS), along with light to activate them, resulting in oxidation of various biological components in cancer tissues. However, since most potential PS are solubilized and given as aqueous solution, PS is non-specifically distributed in the body, leading to the induction of various side effects in normal tissues that are exposed to daylight such as skin and eyes. To overcome the problem associated with non-specific in vivo disposition of PS, various approaches have been applied to develop safer dosage forms for PS with more efficient tumor delivery and lower disposition to normal tissues. Passive drug targeting to tumors with nanoparticulate formulations has been recognized as one of the potentially useful approaches to improve the poor tissue specificity of conventional cancer chemotherapy and this approach should also be applicable for more efficient tumor delivery of PS. In this review article, several issues concerning the efficacy of PDT using nanoparticle-based formulations are discussed and our recent attempts to temporally enhance the vascular permeability within tumors with photodynamic treatment for the better therapeutic outcome of nanoparticle-based therapy are introduced.

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Choice of Oxygen-Conserving Treatment Regimen Determines the Inflammatory Response and Outcome of Photodynamic Therapy of Tumors

Cited by 232 publications

References 27 publications

The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria

The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria

Effect of an oxygen pressure injection (OPI) device on the oxygen saturation of patients during dermatological methyl aminolevulinate photodynamic therapy

Nanoparticle-Based Photodynamic Therapy: Current Status and Future Application to Improve Outcomes of Cancer Treatment

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