Choice of Medium for Cultivation and 24-Hour Incubation of Chorionic Villi: Selective Effects in Vitro

Therkelsen, A. J.; Jensen, Peter K.A.; Hansen, Jørgen Trier; Smidt‐Jensen, Steen; Hahnemann, Niels

doi:10.1007/978-3-642-70707-0_24

Cited by 6 publications

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Direct chromosome preparation and culture using chorionic villi: An evaluation of the two techniques

1990

View full text Add to dashboard Cite

We present a comparison between direct chromosome preparation and cell culture for first trimester fetal chromosome study using chorionic villi. The 2 techniques have advantages and disadvantages and are demonstrated to be appropriate for routine diagnostic work. The combined use of both methods may optimize the quality of the chromosome study and minimize the possibility of false-positive and false-negative findings.

show abstract

Direct chromosome preparation and culture using chorionic villi: An evaluation of the two techniques

1990

View full text Add to dashboard Cite

show abstract

Transabdominal chorionic villus sampling for fetal genetic diagnosis. Technical and obstetrical evaluation of 100 cases

Smidt‐Jensen

Hahnemann

1988

Prenatal Diagnosis

View full text Add to dashboard Cite

First trimester fetal diagnosis was established in 100 pregnancies at risk by transabdominal chorionic villus sampling (TA-CVS). Forty-eight per cent of the women were 35 years or more at the time of sampling. Using the double needle technique, both the aspiration and the diagnostic success rate were 100 per cent. The mean amount of villi aspirated was 28.2 mg (10-50 mg). The mean needle time was 3 min. Vaginal spotting appeared in 2 per cent of the women. Four women had therapeutic abortion due to abnormal findings and one for social reasons. Three fetuses with normal karyotypes were lost. Excluding the therapeutic abortions, the fetal loss rate was 3.2 per cent. The fetal loss rate in the amniocentesis control group (n = 200) was 3.6 per cent. The cytogenetic diagnosis was carried out by the direct preparation technique as well as by chorion villus cultivation. All karyotypes were confirmed by lymphocyte cultures from umbilical cord blood or heel blood from the newborn or from aborted fetal tissue. Transabdominal CVS is deemed a safe and easy tool for achieving chorionic villi in the first trimester.

show abstract

Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester

et al. 1988

Self Cite

View full text Add to dashboard Cite

First trimester prenatal cytogenetic diagnosis was attempted in 350 pregnancies after transabdominal chorionic villus sampling. The cytogenetic investigation was performed using both a short-term method (24 h incubation) and cell culture. Adequate samples were obtained in 99.1 per cent and in all these cases the fetal karyotype was established. A chromosome abnormality was found in 2.0 per cent of cases. A discrepancy between the karyotype obtained after 24 h incubation and the karyotype in cell culture was observed in 2.3 per cent. Maternal cell contamination in the cultures was confirmed in 13 of 181 cases where the 24 h incubation revealed a male karyotype. Studies of culture morphology showed that colonies of convoluted cells may serve as a marker for contamination with maternal cells in culture. For the present, we recommend using a short-term method as well as cell culture for cytogenetic investigation until the problems with karyotype discrepancy and maternal cell contamination have been further clarified.

show abstract

Choice of Medium for Cultivation and 24-Hour Incubation of Chorionic Villi: Selective Effects in Vitro

Cited by 6 publications

References 2 publications

Direct chromosome preparation and culture using chorionic villi: An evaluation of the two techniques

Direct chromosome preparation and culture using chorionic villi: An evaluation of the two techniques

Transabdominal chorionic villus sampling for fetal genetic diagnosis. Technical and obstetrical evaluation of 100 cases

Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester

Contact Info

Product

Resources

About