Chlorpyrifos levels within permitted limits induce nuclear abnormalities and DNA damage in the erythrocytes of the common carp

Mitkovska, Vesela; Chassovnikarova, Tsenka

doi:10.1007/s11356-019-07408-9

Cited by 28 publications

(9 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The toxic influences of CPF are progressively threatening humans and the life of aquatic animals showing several physiological and immunological impacts [10]. CPF toxicity can induce neurobehavioral development by inhibiting the acetylcholinesterase once metabolically activated within the organism [29]. However, the application of functional feed additives can protect fish from the impacts of pesticides, insecticides, and xenobiotics via stimulating the immune and antioxidative responses [30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Spirulina platensis Reduced Oxidative Damage Induced by Chlorpyrifos Toxicity in Nile Tilapia (Oreochromis niloticus)

Abdel-Daim

Dawood

Elbadawy

et al. 2020

Animals

View full text Add to dashboard Cite

Due to the numerous pharmacological impacts of Spirulina platensis (SP), the effects of SP on the oxidative status of Nile tilapia farmed under chlorpyrifos (CPF) ambient toxicity were considered in this study. Fish (60 ± 6.1 g) was randomly stocked in five groups where the SP free diet was fed to the control group while the second one was fed 1% SP without CPF exposure. Additionally, CPF (15 μg/L) was added in water and fish were fed with SP at 0, 0.5, and 1% (third, fourth, and fifth groups, respectively). Samples of blood and gills, kidneys, and liver tissues were assayed for biochemical measurements. Fish exposed to CPF exhibited significant (p ≤ 0.05) increments of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cholesterol, urea, creatinine, and malondialdehyde (MDA), while significantly decreased total protein, albumin, and antioxidative enzyme activities (superoxide dismutase (SOD) and catalase (CAT) were observed in tilapia exposed to CPF (p ≤ 0.05). In contrast, SP feeding resulted in decreased levels of ALT, AST, ALP, cholesterol, urea, and creatinine as well as increased total protein, albumin, SOD, and CAT activities. Based on the obtained results, it can be suggested that SP is efficient in protecting Nile tilapia from CPF toxicity by increasing the antioxidative response.

show abstract

Section: Discussionmentioning

confidence: 99%

Spirulina platensis Reduced Oxidative Damage Induced by Chlorpyrifos Toxicity in Nile Tilapia (Oreochromis niloticus)

Abdel-Daim

Dawood

Elbadawy

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…In addition, four phytosanitary substances (aclonifene, cypermethrine, chlorpyrifos, bifenox) were measured, which are classified as priorities by the WFD. Notably, some of these molecules are potentially genotoxic substances, according to the literature [ 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 ]. Courrières , with high fish mortality, seemed to be the most degraded.…”

Section: Discussionmentioning

confidence: 99%

Integration of Genotoxic Biomarkers in Environmental Biomonitoring Analysis Using a Multi-Biomarker Approach in Three-Spined Stickleback (Gasterosteus aculeatus Linnaeus, 1758)

Cant

Bonnard

Porcher

et al. 2022

Toxics

View full text Add to dashboard Cite

Water is impacted by a variety of increasing pressures, such as contaminants, including genotoxic pollutants. The proposed multi-biomarker approach at a sub-individual level gives a complementary indicator to the chemical and ecological parameters of the Water Framework Directive (WFD, 2000/60/EC). By integrating biomarkers of genotoxicity and erythrocyte necrosis in the sentinel fish species the three-spined stickleback (Gasterosteus aculeatus) through active biomonitoring of six stations of the Artois-Picardie watershed, north France, our work aimed to improve the already existing biomarker approach. Even if fish in all stations had high levels of DNA strand breaks, the multivariate analysis (PCA), followed by hierarchical agglomerative clustering (HAC), improved discrimination among stations by detecting an increase of nuclear DNA content variation (Etaing, St Rémy du Nord, Artres and Biache-St-Vaast) and erythrocyte necrosis (Etaing, St Rémy du Nord). The present work highlighted that the integration of these biomarkers of genotoxicity in a multi-biomarker approach is appropriate to expand physiological parameters which allow the targeting of new potential effects of contaminants.

show abstract

“…Mitkovska and Chassovnikarova [29] stated that CPF causes DNA and nuclear damage in fish, even below the concentrations considered as acceptable by the European legislation. 8-OHdG is a biomarker of oxidative damage caused by free radicals on nuclear and mitochondrial DNA [30].…”

Section: Discussionmentioning

confidence: 99%

Effects of chlorpyrifos on primary gill cell culture of Lake Van fish (Alburnus tarichiGüldenstaadt 1814)

Oğuz

Özok

2020

Toxicology Research

View full text Add to dashboard Cite

Lake Van fish (Alburnus tarichi Guldenstadt 1814) is the only fish that can adapt to the extreme conditions (pH 9.8 salinity 0.2% and alkalinity 151.2 meq/L) of Lake Van. In this study, it was aimed to determine the cytotoxic and genotoxic effects of chlorpyrifos (CPF) on Lake Van fish primary gill cell culture. Gill epithelium from Lake Van fish was isolated enzymatically and grown in primary culture on Leibovitz’s L-15 medium. After different doses (0.01, 0.1, 1, and 10 μM) of CPF were applied to the gill cells, the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), and DNA damage levels (8-hydroxyguanine (8-OHdG)) were examined at the end of 24 and 48 h. It was determined that the TOS, MDA, and 8-OHdG levels increased in the cells exposed to high doses (1 and 10 μM) of CPF and the TAS was decreased (P < 0.05). It was revealed from this study that CPF administered at a dose higher than 1 μM can cause oxidative stress and DNA damage in the primary gill cell culture of Lake Van fish. In addition, the findings showed that Lake Van fish primary gill cell culture was useful in determining the effects of toxic substances likely to be the contaminants of a lake.

show abstract

Chlorpyrifos levels within permitted limits induce nuclear abnormalities and DNA damage in the erythrocytes of the common carp

Cited by 28 publications

References 59 publications

Spirulina platensis Reduced Oxidative Damage Induced by Chlorpyrifos Toxicity in Nile Tilapia (Oreochromis niloticus)

Spirulina platensis Reduced Oxidative Damage Induced by Chlorpyrifos Toxicity in Nile Tilapia (Oreochromis niloticus)

Integration of Genotoxic Biomarkers in Environmental Biomonitoring Analysis Using a Multi-Biomarker Approach in Three-Spined Stickleback (Gasterosteus aculeatus Linnaeus, 1758)

Effects of chlorpyrifos on primary gill cell culture of Lake Van fish (Alburnus tarichiGüldenstaadt 1814)

Contact Info

Product

Resources

About