Chlorpromazine-Impregnated Catheters as a Potential Strategy to Control Biofilm-Associated Urinary Tract Infections

Sidrim, J. J. C.; Amando, Bruno Rocha; Gomes, Francisco I. R.; Amaral, Marilia Smg do; Sousa, Paulo Cp de; Ocadaque, Crister José; Brilhante, Raimunda Sn; Cordeiro, Rossana de Aguiar; Rocha, Marcos F.G.; Castelo-Branco, Débora de Souza Collares Maia

doi:10.2217/fmb-2019-0092

Cited by 18 publications

(11 citation statements)

References 35 publications

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“…K. pneumoniae is a major nosocomial infection, found worldwide, that shows resistance to many commonly used antibiotics and has the ability to form biofilms on different physiological surfaces and medical devices [27][28][29][30][31]. Hence, there is a need for inhibition of the bacterial biofilm formation to control the infection, while, biologically synthesized NPs are new agents with antibiofilm activity [32].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

et al. 2020

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The aim of the present work was to investigate the antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles (AgNPs) fabricated from Mespilus germanica extract against multidrug‐resistant (MDR) Klebsiella pneumoniae strains. Fifty strains of K. pneumoniae were isolated from various clinical specimens. Biofilm‐forming strains were identified using Congo red agar and polymerase chain reaction (PCR) techniques. Subsequently, the antibacterial activity of phytosynthesized AgNPs on MDR K. pneumoniae strains was investigated by broth microdilution assay and agar well‐diffusion method. Finally (in the last step), the antibiofilm activity of phytosynthesized AgNPs was determined using microtiter plate assay and real‐time PCR (RT‐PCR) methods for the analysis of type 3 fimbriae (mrkA) and quorum‐sensing system (luxS) gene expression. The results of this study showed that the phytosynthesized AgNPs had a spherical nanostructure with the mean size of 17.60 nm. The AgNPs exhibited dose‐dependent antibacterial activity. The results of the microtiter plate and RT‐PCR methods show that AgNPs inhibited the biofilm formation in MDR K. pneumoniae strains, and the expressions of mrkA and luxS genes were downregulated significantly in MDR strains after treatment with a subminimum inhibitory concentration of AgNPs. In conclusion, AgNPs effectively prevent the formation of biofilms and kill bacteria in established biofilms, which suggests that AgNPs might be a promising candidate for the prevention and treatment of biofilm‐related infections caused by MDR K. pneumoniae strains.

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Section: Discussionmentioning

confidence: 99%

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

et al. 2020

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“…Furthermore, docking studies demonstrated that the enhanced inhibitory action of the irradiated compound was a result of the overall effect of the photoproducts on the biological target. The capability of chlorpromazine to impede the biofilm formation on urinary catheter was also demonstrated by Sidrim et al [ 59 ] in a previous work, in which this drug significantly suppressed the growth of E. coli , Proteus mirabilis , and Klebsiella. Tozar et al in 2020 examined the anti-staphylococcal activity and mode of action of thioridazine photoproducts after different periods of laser irradiation.…”

Section: Antidepressants and Antipsychoticsmentioning

confidence: 56%

Non-Antibiotic Drug Repositioning as an Alternative Antimicrobial Approach

et al. 2022

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The worldwide scenario of antibiotic resistance and the falling number of funds for the development of novel antibiotics have led research efforts toward the study of specific cost-effective strategies aimed at discovering drugs against microbial infections. Among the potential options, drug repositioning, which has already exhibited satisfactory results in other medical fields, came out as the most promising. It consists of finding new uses for previously approved medicines and, over the years, many “repurposed drugs” displayed some encouraging in vitro and in vivo results beyond their initial application. The principal theoretical justification for reusing already existing drugs is that they have known mechanisms of action and manageable side effects. Reuse of old drugs is now considered an interesting approach to overcome the drawbacks of conventional antibiotics. The purpose of this review is to offer the reader a panoramic view of the updated studies concerning the repositioning process of different classes of non-antibiotic drugs in the antimicrobial field. Several research works reported the ability of some non-steroidal anti-inflammatory drugs (NSAIDs), antidepressants, antipsychotics, and statins to counteract the growth of harmful microorganisms, demonstrating an interesting winning mode to fight infectious diseases caused by antimicrobial resistant bacteria.

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“…Killing effects of antibiotics are known to be dramatically reduced in the presence of foreign bodies such as sutures and implants because bacteria form biofilms on the foreign bodies [21]. For example, catheter associated urinary tract infections are of serious concern [22]. Cells in biofilms are slow growing and thus are more resistant to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic in Myrrh From Commiphora Molmol Preferentially Kills Nongrowing Bacteria

Bhattacharjee

Alenezi

2020

Future Sci. OA

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Aim: To demonstrate that myrrh oil preferentially kills nongrowing bacteria and causes no resistance development. Method: Growth inhibition was determined on regular plates or plates without nutrients, which were later overlaid with soft agar containing nutrients to continue growth. Killing experiments were done in broth and in buffer without nutrients. Results: Bacterial cells were inhibited preferentially in the absence of nutrients or when growth was halted by a bacteriostatic antibiotic. After five passages in myrrh oil, surviving colonies showed no resistance to the antibiotic. Conclusion: Myrrh oil has the potential to be a commercially viable antibiotic that kills persister cells and causes no resistance development. This is a rare example of an antibiotic that can preferentially kill nongrowing bacteria.

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Chlorpromazine-Impregnated Catheters as a Potential Strategy to Control Biofilm-Associated Urinary Tract Infections

Cited by 18 publications

References 35 publications

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

Non-Antibiotic Drug Repositioning as an Alternative Antimicrobial Approach

Antibiotic in Myrrh From Commiphora Molmol Preferentially Kills Nongrowing Bacteria

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