Chloroplast and nuclear DNA studies in a few members of the Brassica oleracea L. group using PCR-RFLP and ISSR-PCR markers: a population genetic analysis

Abstract

A population genetic analysis of chloroplast and nuclear DNA was performed covering nine wild populations of Brassica oleracea. Three members of the n = 9 group, all close to B. oleracea, Brassica alboglabra Bailey, Brassica bourgeaui (Webb) O. Kuntze and Brassica montana Pourret, were also studied to better understand their relationship with B. oleracea. Chloroplast DNA was analysed using the PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) method. The ISSR-PCR (inter-simple sequence repeat - polymerase chain reaction) technique was adopted to study nuclear DNA. Twelve primer pairs of chloroplast DNA showed very good amplification. The amplified product of each primer pair, digested by three restriction enzymes, revealed no variation of cpDNA among the taxa studied. This indicates they may have the same chloroplast genotype. Seven selected ISSR primers have detected genetic variation, both within and among the populations/taxa surveyed. The information obtained on the intra- and inter-populational genetic diversity of wild populations of B. oleracea neatly defined the individual plants. It could provide important guidelines for backing management and conservation strategies in this species. The study confirms a close relationship between B. alboglabra, B. bourgeaui and B. montana, which is parallel to their morphological similitude.

Genetic Relationship of Sago Palm (Metroxylon sagu Rottb.) in Indonesia Based on RAPD Markers

“…In our observation showed that the different genetic markers used did not change grouping pattern of sago palm. Some cases in the molecular analysis, the dissimilarities grouping pattern, by using the same markers or different markers, were found frequently in the studied of genetic relationships (Ishikawa et al, 1992;Viard et al, 2001;Panda et al, 2003). …”

“…Therefore, RAPD markers are molecular markers which it have no longer conservative periods time rather than cpDNA markers. In the previous studies at different plants showed that higher variation were found by using nuclear genome markers (RAPD, AFLP, ISSR, and nuclear SSR), then using chloroplast genome markers such as cpDNA markers (Hultquist, 1996;Viard et al, 2001;Cronn et al, 2002;Panda et al, 2003). …”

Relationships among Avena species as revealed by consensus chloroplast simple sequence repeat (ccSSR) markers

“…As a result, chloroplast-specific markers are considered good indicators of historical bottlenecks, founder effects and genetic drift. Chloroplasts evolve slowly and exhibit little variation at the intraspecific level (Clegg et al 1994;Provan et al 2001;Panda et al 2003). The chloroplast DNA (cpDNA) markers, have been widely used for phylogenetic inference (Olmstead and Palmer 1994) and to some extent, for within-species genetic studies (Soltis et al 1992;Ennos et al 1999).…”

DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs

“…ISSR markers have been used for cultivar identification in maize (Kantety et al, 1995;Pejic et al, 1998), trifolioate orange (Fang & Roese, 1997), wheat (Nagaoka & Ogihara, 1997), potatoes (Prevost & Wilkinson, 1999), Oryza (Blair et al, 1999;Joshi et al, 2000;Qian et al, 2001), Diplotaxis (Martín & Sánchez-Yélamo, 2000) bean (Métais et al, 2000), rye (Matos et al, 2001), barley (Fernández et al, 2002), chickpea (Iruela et al, 2002), Brassica (Panda et al, 2003), cucurbita (Paris et al, 2003) and almonds (Martins et al, 2003).…”