Chlorophyllase Activity and Chlorophyll Content in Wild and Mutant Plants of Arabidopsis thaliana

Todorov, D.; Karanov, E.; Smith, A. R.; Hall, Michael

doi:10.1023/a:1022355525907

Cited by 28 publications

(19 citation statements)

References 14 publications

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“…The detrimental effects of heat on chlorophyll and the photosynthetic apparatus are also associated with the production of injurious reactive oxygen species (ROSs; Camejo et al, 2006; Guo et al, 2007). By increasing chlorophyllase activity and decreasing the amount of photosynthetic pigments, heat stress ultimately reduces the plant photosynthetic and respiratory activity (Todorov et al, 2003; Sharkey and Zhang, 2010). With respect to reproductive success, a decline of photosynthesis will eventually result in limited resource availability for reproduction in parental and gametophytic tissues due to a reduction in energy reserves leading to plant starvation (Young et al, 2004; Sumesh et al, 2008).…”

Section: Molecular Cellular and Physiological Impact Of Heat Stressmentioning

confidence: 99%

Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops

Bita¹,

Geräts²

2013

Front. Plant Sci.

1,358

924

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Global warming is predicted to have a general negative effect on plant growth due to the damaging effect of high temperatures on plant development. The increasing threat of climatological extremes including very high temperatures might lead to catastrophic loss of crop productivity and result in wide spread famine. In this review, we assess the impact of global climate change on the agricultural crop production. There is a differential effect of climate change both in terms of geographic location and the crops that will likely show the most extreme reductions in yield as a result of expected extreme fluctuations in temperature and global warming in general. High temperature stress has a wide range of effects on plants in terms of physiology, biochemistry and gene regulation pathways. However, strategies exist to crop improvement for heat stress tolerance. In this review, we present recent advances of research on all these levels of investigation and focus on potential leads that may help to understand more fully the mechanisms that make plants tolerant or susceptible to heat stress. Finally, we review possible procedures and methods which could lead to the generation of new varieties with sustainable yield production, in a world likely to be challenged both by increasing population, higher average temperatures and larger temperature fluctuations.

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Section: Molecular Cellular and Physiological Impact Of Heat Stressmentioning

confidence: 99%

Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops

Bita¹,

Geräts²

2013

Front. Plant Sci.

1,358

924

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“…Enzyme extraction. The procedure of Chlase isolation was carried out using a modified method described by Todorov et al (2003a). Frozen leaf tissues (30 g) were homogenized at 4ºC for 5 min.…”

Section: Plant Materials Leaves Of Three Representatives Ofmentioning

confidence: 99%

“…It has been elucidated that young leaves of tea and tobacco possess higher levels of Chlase activity when compared to their respective mature organs (Kuroki et al, 1981;Todorov et al, 2003b). Additionally, Todorov et al (2003a) revealed that upper rosette leaves of Arabidopsis thaliana are characterised by lower activity of Chlase and higher amounts of chlorophylls in relation to the inferior ones. It should be underlined that many authors reported an elevated activity of Chlase in plants subjected to a wide spectrum of environmental stressors such as water or manganese deficiency (Majumdar et al, 1991;Saidi et al, 2012), heavy metal exposure (Mihailovic et al, 2008), low and high temperature stress (Johnson-Flanagan & McLachlan, 1990;Todorov et al, 2003b), aphid infestation (Ciepiela et al, 2005), and phytopathogenic infections and wounding (Stangarlin & Pascholati, 2000;Kariola et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…It should be underlined that many authors reported an elevated activity of Chlase in plants subjected to a wide spectrum of environmental stressors such as water or manganese deficiency (Majumdar et al, 1991;Saidi et al, 2012), heavy metal exposure (Mihailovic et al, 2008), low and high temperature stress (Johnson-Flanagan & McLachlan, 1990;Todorov et al, 2003b), aphid infestation (Ciepiela et al, 2005), and phytopathogenic infections and wounding (Stangarlin & Pascholati, 2000;Kariola et al, 2005). Interestingly, Todorov et al (2003a) demonstrated higher Chlase activity in leaves of the ethylene-insensitive mutant (eti5) of A. thaliana when compared to the wild-type plants. Furthermore, changes in the enzyme activity in response to low and high temperature stress were more significant in leaves of eti5 mutants in relation to the wild-type ones (Todorov et al, 2003b).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study.

Sytykiewicz

Sprawka²,

Czerniewicz³

et al. 2013

Acta Biochim Pol

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Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolase, EC 3.1.1.14) from leaves of three Prunus species: bird cherry (Prunus padus L.), European plum (Prunus domestica L.), and sour cherry (Prunus cerasus L.). Secondarily, this report was aimed at comparing seasonal dynamics of Chlase activity and chlorophyll a (Chl a) content within investigated plant systems. Molecular weight of native Chlase F1 has been estimated at 90 kDa (bird cherry) and approximately 100 kDa (European plum and sour cherry), whereas molecular mass of Chlase F2 varied from 35 kDa (European plum) to 60 kDa (sour cherry). Furthermore, enzyme fractions possessed similar optimal pH values ranging from 7.6 to 8.0. It was found that among a broad panel of tested metal ions, Hg(+2), Fe(+2), and Cu(+2) cations showed the most pronounced inhibitory effect on the activity of Chlase. In contrast, the presence of Mg(+2) ions influenced a subtle stimulation of the enzymatic activity. Importantly, although Chlase activity was negatively correlated with the amount of Chl a in leaves of examined Prunus species, detailed comparative analyses revealed an incidental decrement of enzymatic activity in early or moderately senescing leaves. It provides evidence that foliar Chlase is not the only enzyme involved in autumnal chlorophyll breakdown and further in-depth studies elucidating this catabolic process are required.

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“…With the aim of detecting the potential benefits of plant inoculation with two different AMF species in a heat tolerant grapevine variety exposed to elevated temperatures, in the present work, several methods were compared. Leaf chlorophyll content and electrolyte leakage have traditionally been used as indirect measures of heat stress tolerance in diverse plant species [33,[82][83][84][85][86], as high stressful temperatures commonly lead to a decrease in the amount of photosynthetic pigments [85,87,88] and also affect the integrity and functions of biological membranes, enhancing their permeability and leading to cell electrolyte leakage [9]. However, exposure of 'Touriga Nacional' grapevine plants to high temperatures for five days led to a general increase in ChlC and a decrease in EL in mature leaves (Tables 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Response of Mycorrhizal ’Touriga Nacional‘ Variety Grapevines to High Temperatures Measured by Calorespirometry and Near-Infrared Spectroscopy

Nogales

Ribeiro

Nogales-Bueno

et al. 2020

Plants

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Heat stress negatively affects several physiological and biochemical processes in grapevine plants. In this work, two new methods, calorespirometry, which has been used to determine temperature adaptation in plants, and near-infrared (NIR) spectroscopy, which has been used to determine several grapevine-related traits and to discriminate among varieties, were tested to evaluate grapevine response to high temperatures. ‘Touriga Nacional’ variety grapevines, inoculated or not with Rhizoglomus irregulare or Funneliformis mosseae, were used in this study. Calorespirometric parameters and NIR spectra, as well as other parameters commonly used to assess heat injury in plants, were measured before and after high temperature exposure. Growth rate and substrate carbon conversion efficiency, calculated from calorespirometric measurements, and stomatal conductance, were the most sensitive parameters for discriminating among high temperature responses of control and inoculated grapevines. The results revealed that, although this vine variety can adapt its physiology to temperatures up to 40 °C, inoculation with R. irregulare could additionally help to sustain its growth, especially after heat shocks. Therefore, the combination of calorespirometry together with gas exchange measurements is a promising strategy for screening grapevine heat tolerance under controlled conditions and has high potential to be implemented in initial phases of plant breeding programs.

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Chlorophyllase Activity and Chlorophyll Content in Wild and Mutant Plants of Arabidopsis thaliana

Cited by 28 publications

References 14 publications

Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops

Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study.

Response of Mycorrhizal ’Touriga Nacional‘ Variety Grapevines to High Temperatures Measured by Calorespirometry and Near-Infrared Spectroscopy

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