Chlorophenol tolerant and degradative bacteria isolated from a river receiving pulp mill discharges

Martı́nez, Miguel Ángel; Baeza, Jaime; Freer, Juanita; Rodrı́guez, Jaime

doi:10.1080/02772240009358947

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…The environmental fate of chlorophenols may include degradation by naturally occurring microorganisms (Aranda et al 1999;Martinez et al 2000). Anaerobic microorganisms are able to degrade chlorophenolic compounds but in anaerobic conditions, multichlorophenols are degraded by reductive dehalogenation into mono-or dichlorophenols which remain in the environment because of low degradation rates (Mohn & Kennedy 1992;Fahmy et al 1994;Chang et al 1999).…”

Section: Introductionmentioning

confidence: 98%

“…2,4,6-TCP is a common constituent of wood preservative formulations (Chudoba et al 1989;Aranda et al 1999;Martinez et al 2000) and is often found as a contaminant in soil (Dominguez et al 2002). 2,4,6-TCP has a low Henry's law constant and as such undergoes minimal air stripping in forced aeration systems.…”

Section: Introductionmentioning

confidence: 99%

“…Aquatic environments may contain chlorophenols resulting from the Kraft bleaching process (Alexander & Scow 1989;Andreoni et al 1998;Martinez et al 2000) or chlorination of water supplies for the purpose of disinfection (Ahlborg & Thunberg 1980).…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation kinetics of 2,4,6-Trichlorophenol by an acclimated mixed microbial culture under aerobic conditions

et al. 2006

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The objective of this study was to achieve a better quantitative understanding of the kinetics of 2,4,6-trichlorophenol (TCP) biodegradation by an acclimated mixed microbial culture. An aerobic mixed microbial culture, obtained from the aeration basin of the wastewater treatment plant, was acclimated in shake flasks utilizing various combinations of 2,4,6-TCP (25-100 mg l(-1)), phenol (300 mg l(-1)) and glycerol (2.5 mg l(-1)) as substrates. Complete primary TCP degradation and a corresponding stoichiometric release of chloride ion were observed by HPLC and IEC analytical techniques, respectively. The acclimated cultures were then used as an inoculum for bench scale experiments in a 4 l stirred-tank reactor (STR) with 2,4,6-TCP as the sole carbon/energy (C/E) source. The phenol acclimated mixed microbial culture consisted of primarily Gram positive and negative rods and was capable of degrading 2,4,6-TCP completely. None of the predicted intermediate compounds were detected by gas chromatography in the cell cytoplasm or supernatant. Based on the disappearance of 2,4,6-TCP, degradation was well modelled by zero-order kinetics which was also consistent with the observed oxygen consumption. Biodegradation rates were compared for four operating conditions including two different initial 2,4,6-TCP concentrations and two different initial biomass concentrations. While the specific rate constant was not dependent on the initial 2,4,6-TCP concentration, it did depend on the initial biomass concentration (X (init)). A lower biomass concentration gave a much higher zero-order specific degradation rate. This behaviour was attributed to a lower average biomass age or cell retention time (theta(x)) for these cultures. The implications of this investigation are important for determining and predicting the potential risks associated with TCP, its degradation in the natural environment or the engineering implications for ex situ treatment of contaminated ground water or soil.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Biodegradation kinetics of 2,4,6-Trichlorophenol by an acclimated mixed microbial culture under aerobic conditions

et al. 2006

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“…The environmental fate of chlorophenols may include degradation by naturally occurring microorganisms (Aranda et al, 1999;Martinez et al, 2000). Several different models have been proposed for the biodegradation of different chlorophenolics (Armenante et al, 1995;Chen et al, 2003;Crosby, 1981;Dominguez et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Their toxicity and persistence (Armenante et al, 1995) have led to their detection in soils, sediments and natural water (Gardin et al, 2001) as well as in the food chain. Aquatic environments may contain chlorophenols resulting from the kraft bleaching process (Martinez et al, 2000) or chlorination of water supplies for the purpose of disinfection (Ahlborg and Thunberg, 1980). The pulp and paper mill effl uent contains diverse kinds of pollutants.…”

Section: Introductionmentioning

confidence: 99%

Degradation of 2,4,6-trichlorophenol by bacteria isolated from secondary sludge of a pulp and paper mill

Karn

Reddy

2012

J. Gen. Appl. Microbiol.

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Bacterial strains capable of degrading trichlorophenol (2,4,6-TCP) were isolated from the secondary sludge of a pulp and paper mill and were characterized. These isolates were identifi ed as Planococcus rifi etoensis (CL4) and Bacillus pumilus (CL5), based on their 16S rRNA sequence analysis. These isolates were able to grow and utilize 2,4,6-TCP as their source of carbon as well as energy. HPLC analysis and stoichometric release of chloride in the medium confi rmed the degradation ability of these isolates. Removal effi ciency of 2,4,6-TCP by these isolates was discovered to be high. They were able to remove 90% of 2,4,6-TCP when grown at a concentration of 600 mg L 1 . Inoculation of these bacteria completely removed 2,4,6-TCP within 2 weeks from the sludge of the pulp and paper mill when supplemented at the rate of 100 mg L 1 . Absorbable Organic Halogen (AOX) and Extractable Organic Halogen (EOX) were signifi cantly reduced by 63% and 70% respectively from the sludge due to inoculation of these bacteria. These isolates have high potential to remove 2,4,6-TCP and may be used for removal of 2,4,6-TCP from pulp paper mill waste.

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Degradative ability of 2,4,6-tribromophenol by saprophytic fungi Trametes versicolor and Agaricus augustus isolated from chilean forestry

Donoso

Becerra

Martı́nez

et al. 2007

World J Microbiol Biotechnol

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Trametes versicolor and Agaricus augustus, with a maximum tolerable concentration (MTC) of 80 lg ml -1 tribromophenol (TBP), were selected to evaluate TBP biodegradation capacity. These fungi were capable of decreased TBP concentrations and A. augustus was also capable of biotransforming TBP to tribromoanisole (TBA). Peroxidase and laccase activities were observed in the T. versicolor supernatant but not in that of A. augustus. These tolerance levels could be due to either lignolytic enzymes that degrade TBP or the ability of the fungi to biotransform TBP to tribromoanisole, respectively. The sustained ability of T. versicolor to degrade TBP (total of 40 lg ml -1 ) in the presence of an additional carbon source suggests that it may have potential applications in the degradation of forestry industry waste.

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Chlorophenol tolerant and degradative bacteria isolated from a river receiving pulp mill discharges

Cited by 7 publications

References 23 publications

Biodegradation kinetics of 2,4,6-Trichlorophenol by an acclimated mixed microbial culture under aerobic conditions

Biodegradation kinetics of 2,4,6-Trichlorophenol by an acclimated mixed microbial culture under aerobic conditions

Degradation of 2,4,6-trichlorophenol by bacteria isolated from secondary sludge of a pulp and paper mill

Degradative ability of 2,4,6-tribromophenol by saprophytic fungi Trametes versicolor and Agaricus augustus isolated from chilean forestry

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