Chlorogenic Acid Stimulates the Thermogenesis of Brown Adipocytes by Promoting the Uptake of Glucose and the Function of Mitochondria

Han, Xue; Zhang, Yuxin; Guo, Jielong; You, Yilin; Zhan, Jicheng; Huang, Weidong

doi:10.1111/1750-3841.14838

Cited by 30 publications

(23 citation statements)

References 40 publications

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“…The culture and induction protocol of the C3H10T1/2 were the same as reported previously. [ 19 ] The cells were cultured in growth medium consisting of high‐glucose DMEM containing 10% fetal bovine serum and antibiotics. The cells were cultured in a 12‐well plate, and the medium was replaced with induction medium when they reached 80% (day 0).…”

Section: Methodsmentioning

confidence: 99%

Grape Extract Activates Brown Adipose Tissue Through Pathway Involving the Regulation of Gut Microbiota and Bile Acid

Han

Guo

Yin

et al. 2020

Molecular Nutrition Food Res

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Scope: Although the physiological function of grape extract (GE) has long been recognized, the precise mechanism remains obscure. This study is designed to investigate the effects of GE on metabolism and the association between GE activation of brown adipose tissue (BAT) and the restoration of gut microbiota (GM). Methods and results: Diet-induced obese mice are used to investigate the function of GE. GE administration increases energy metabolism and prevents obesity. Also, GE restores the dysbiosis of GM by augmenting the observed species, enhancing the Firmicutes-to-Bacteroidetes ratio and increasing the abundance of the Bifidobacteria, Akkermansia, and Clostridia genera. This restoration of GM alters the bile acid (BA) pool in the serum. The abundance of Akkermansia, Clostridium, and Bifidobacterium is negatively correlated with the concentrations of T MCA, T MCA, and TCA but is positively correlated with DCA. The changes in BA promoted TGR5 in BAT, which contributed to thermogenesis. The metabolites of GE in blood do not stimulate TGR5 in vitro. Conclusion: GE stimulates the thermogenesis of BAT through a pathway involving the regulation of GM and BA in diet-induced obese mice. This study reveals the mechanism by which dietary polyphenols promote thermogenesis by regulating BA, which is altered by GM. ments; X.H. wrote the paper.

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Section: Methodsmentioning

confidence: 99%

Grape Extract Activates Brown Adipose Tissue Through Pathway Involving the Regulation of Gut Microbiota and Bile Acid

Han

Guo

Yin

et al. 2020

Molecular Nutrition Food Res

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“…The cytotoxicity of CA on C3H10T1/2 after a period of 48 hours was detected using the Cell Counting Kit‐8 (CCK8, Beyotime Biotechnology, Shanghai, China), according to the manufacturer's instructions. The oil red O staining of mature brown adipocytes on Day 6 was performed as we described before 25 …”

Section: Methodsmentioning

confidence: 99%

“…Oxygen consumption rate (OCR) measurement was performed on the C3H10T1/2 with the Agilent Seahorse XF24 cellular respirometer, as previously described 25 . Briefly, mitochondrial stress tests were performed on 6000 cells/well in V24 microplates.…”

Section: Methodsmentioning

confidence: 99%

p‐Coumaric acid prevents obesity via activating thermogenesis in brown adipose tissue mediated by mTORC1‐RPS6

et al. 2020

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Brown adipose tissue (BAT) has long been recognized as an energy‐consuming organ and a possible target for combating metabolism disorder. Although numerous studies have demonstrated the ability of phytochemical phenolic acids to improve obesity by activating BAT, the underlying mechanism or mechanism therein remain obscure. In this study, diet‐induced obese mice, genetically obese mice, and C3H10T1/2 cells were used to examine the effects of p‐Coumaric acid (CA) on metabolism profiles. The results showed that CA prevented metabolic syndromes in the two mice models through the activation of BAT. This phenomenon was closely linked to the upregulation of uncoupling protein 1 (UCP1) and the accelerated burning of fatty acids and glucose, which consequently enhanced the energy expenditure and thermogenesis. Similar results were also obtained in vitro. Importantly, these effects were mediated by the mammalian target of rapamycin complex 1 (mTORC1)‐RPS6 pathway. These findings reveal, to the best of our knowledge for the first time, the close correlation between mTORC1‐RPS6 and BAT‐mediated thermogenesis, and, in addition, the key role played by mTORC1‐RPS6 in mediating phenolic acids‐induced activation of BAT, thus preventing obesity.

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“…From a medicinal point of view, chlorogenic acid (CGA) and other related compounds such as isomers, derivatives and/or other quinic acid conjugates are potent antioxidants. Moreover, there is some evidence that CGAs have antihypertensive [ 4 ], anti-obesity, and anti-diabetic activity through positive effects on glucose and lipid metabolism and through the thermogenesis stimulation of brown adipocytes [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Helichrysum italicum ssp. italicum Infusion Promotes Fat Oxidation in Hepatocytes and Stimulates Energy Expenditure and Fat Oxidation after Acute Ingestion in Humans: A Pilot Study

Kenig

Kramberger

Petelin

et al. 2021

Plants

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Helichrysum italicum is an aromatic plant with promising pharmacological activities. Bioactive compounds found in plants represent an important alternative treatment for weight loss and an infusion of H. italicum contains compounds which could have such effect. Our aim was, therefore, to investigate its acute effects on resting energy expenditure (REE) and possible differences in substrate oxidation in a pilot study. A dried, ground plant material of H. italicum ssp. italicum was infused with hot water and chemically characterized with HPLC-MS analysis. Sensory evaluation of herbal tea was performed. A randomized, crossover, controlled pilot study was then conducted on eleven healthy male subjects. The REE and substrate oxidation were measured by indirect calorimetry at baseline and 30 and 120 min after ingestion of infusion or hot water. The expression of genes involved in lipid metabolism was examined in H. italicum infusion-treated hepatocytes. Several phenolic classes were identified in the infusion, caffeoylquinic acids were the most abundant, followed by pyrones and flavonols. A single ingestion of H. italicum infusion significantly increased REE by 4% and fat oxidation by 12% compared to hot water ingestion. A significant 2-fold up-regulation of β-oxidation-related genes in HepG2 cells, exposed to H. italicum infusion, was detected. This pilot study suggests that H. italicum infusion possesses bioactive substances with potential application in obesity prevention, which could, with additional studies, become an economically interesting novel application of the plant. Clinical trial registration number: NCT04818905

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Chlorogenic Acid Stimulates the Thermogenesis of Brown Adipocytes by Promoting the Uptake of Glucose and the Function of Mitochondria

Cited by 30 publications

References 40 publications

Grape Extract Activates Brown Adipose Tissue Through Pathway Involving the Regulation of Gut Microbiota and Bile Acid

Grape Extract Activates Brown Adipose Tissue Through Pathway Involving the Regulation of Gut Microbiota and Bile Acid

p‐Coumaric acid prevents obesity via activating thermogenesis in brown adipose tissue mediated by mTORC1‐RPS6

Helichrysum italicum ssp. italicum Infusion Promotes Fat Oxidation in Hepatocytes and Stimulates Energy Expenditure and Fat Oxidation after Acute Ingestion in Humans: A Pilot Study

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