Chlorhexidine-encapsulated mesoporous silica-modified dentin adhesive

Yan, Hui-yi; Wang, Shilei; Han, Lin; Peng, Wenan; Yi, Luyao; Guo, Rui; Liu, Siying; Yang, Hongye; Huang, Cui

doi:10.1016/j.jdent.2018.08.012

Cited by 34 publications

(31 citation statements)

References 44 publications

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“…When MSN were examined under SEM and TEM/EDX (Figure 2), the nanoparticles appeared smooth with regular mesoporosity. The mesoporosity became irregular and less clearly de ned after loading of CHX signifying the adsorption of the drug onto the external surface of MSN and inside the mesoporous cavities [9]. EDS elemental mapping indicated strong intra-particulate silicon, carbon, and chlorine in CHX-loaded/MSN-PLGA nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…Preparation of MSN-PLGA and chlorhexidine loading MSN-PBLG of 0.1 g was dispersed in 10 mL tri uoroacetic acid (TFA) in an ice bath and HBr (1 mL, 33 wt% in acetic acid) was added dropwise within 10 min and stirred for 2 h. The reaction mixture was poured into 40 mL ice-cold dry ether, followed by centrifugation, washing with distilled water, and drying over-night under vacuum at 45 °C for 12 h, to form white powder of MSN-PLGA. The incorporation of CHX into MSN and MSN-PLGA was adopted and modi ed from previous protocols [9,13]. In brief, 25 and 50 mg of CHX was dissolved in 5 mL of dichloromethane added with 50 mg of MSN or MSN-PLGA, respectively, and sonicated for 12 min and slowly shaken at room temperature for 24 h, followed by centrifugation and vacuum drying.…”

Section: Synthesis Of Poly(γ-benzyl-l -Glutamate) Grafted Over Msn (Mmentioning

confidence: 99%

“…To test micro-tensile bond strength (µ-TBS), 5 wt.% of all the nanoparticles were added separately in the commercial adhesive (Scotchbond TM bond, 3M ESPE, USA) [9]. The restored teeth were sectioned using a low-speed diamond saw (Buehler, Lake Bluff, IL, USA) under water coolant into resin-dentin beams (0.9 x 0.9 mm) and stored in arti cial saliva (pH 7.4) for one week.…”

Section: Micro-tensile Bond Strengthmentioning

confidence: 99%

“…In the recent years, porous materials have offered a promising therapeutic solution to a wide variety of elds [8]. Of these, mesoporous silica nanoparticles (MSNs) are a category of porous materials with exceptional surface area and signi cant number of pores that load various quantities of drugs within for improved e cacy [9,10]. Moreover, these synthesized structures can undergo surface modi cations, possess excellent biocompatibility and high pore volume.…”

Section: Introductionmentioning

confidence: 99%

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pH Dependent Delivery of Chlorhexidine From PLGA Grafted Mesoporous Silica Nanoparticles at Resin-Dentin Interface

Akram

Aati

Ngo

et al. 2020

Preprint

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Background: A low pH environment is created due to the production of acids by oral biofilms that further leads to the dissolution of hydroxyapatite crystal in the tooth structure significantly altering the equilibrium. Although the overall bacterial counts may not be eradicated completely from the oral cavity, however, synthesis of engineered anti-bacterial materials are warranted in order to reduce the pathogenic impact of the oral biofilms. The purpose of this study was to synthesize and characterize chlorhexidine-loaded mesoporous silica nanoaprticles (MSN) grafted with poly-L-glycolic acid (PLGA) and to test the in vitro drug release in various pH environments, cytotoxicity, and antimicrobial capacity. In addition, to investigate the delivery of CHX-loaded/MSN-PLGA through dentin tubules of demineralized dentin substrates and the interaction of these nanoparticles with commercial dentin adhesive after applying it on demineralized substrates for potential clinical application.Results: Characterization using SEM/TEM and EDX confirmed the synthesis of CHX-loaded/MSN-PLGA. An increase in the percentage of drug encapsulation efficiency from 81% to 85% in CHX loaded/MSN and 92% to 95% in CHX loaded/MSN-PLGA proportionately increased with increasing the amount of CHX during the fabrication of nanoparticles. For both time-periods (24 h or 30 Days), the relative microbial viability significantly decreased by increasing the CHX content (P<0.001). Generally, the cell viability percentage of DPSCs exposed to MSN-PLGA/Blank, CHX-loaded/MSN, and CHX-loaded/MSN-PLGA, respectively was >80% indicating low cytotoxicity profiles of experimental nanoparticles. After 9 months in artificial saliva (pH 7.4), significantly highest micro-tensile bond strength value was recorded for 25:50 CHX/MSN and 25:50:50 CHX/MSN-PLGA. A homogenous and widely distributed 50:50:50 CHX-loaded/MSN-PLGA nanoparticles exhibited excellent bonding with the application of commercially available dentin adhesive.Conclusions: A pH sensitive CHX release response was noted when loaded in MSN grafted PLGA nanoparticles. The formulated drug loaded nanocarrier demonstrated excellent physicochemical, spectral, and biological characteristics. Showing considerable capacity to penetrate effectively inside dentinal tubules and having high antibacterial efficacy, this system could be potentially used in adhesive and restorative dentistry.

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Section: Discussionmentioning

confidence: 99%

Section: Synthesis Of Poly(γ-benzyl-l -Glutamate) Grafted Over Msn (Mmentioning

confidence: 99%

Section: Micro-tensile Bond Strengthmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

pH Dependent Delivery of Chlorhexidine From PLGA Grafted Mesoporous Silica Nanoparticles at Resin-Dentin Interface

Akram

Aati

Ngo

et al. 2020

Preprint

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“…For enzyme-mediated aging, another 10 bonded teeth were used. Sectioned bonding specimens were immersed in 2 mL 2 mg/mL matrix metalloproteinase 8 (MMP-8) solution for 72 h before evaluating the microtensile bond strength (Yan et al 2018).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Collagen-Reactive Monomer with Advanced Bonding Durability

Fan

Luo

et al. 2020

J Dent Res

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This study evaluated the use of a new collagen-reactive monomer (CRM), isocyanate-terminated urethane methacrylate precursor, which has covalent affinity to dental collagen, in the formation of dentin-resin bonds and compared it with 2 other dental adhesives. Dentin specimens were bonded with either the CRM-based adhesive (CBA), One-Step (OS; Bisco, Inc.), or a negative adhesive (NA) control and subjected to 24-h storage in water, thermocycling to simulate 1-y clinical function, or a matrix metalloproteinase–mediated aging process. We tested the microtensile bond strength (µTBS), characterized the bonding interface with an atomic force microscope, conducted micro-Raman analysis, and performed leakage tests and in situ zymography. CBA and OS exhibited comparable bonding strength after 24 h ( P > 0.05); however, there was a sharp decrease in µTBS after aging for all except CBA ( P < 0.001). Raman spectra results indicated increased collagen crosslinking and chemical reaction between the adhesive and collagen in the CBA group. CBA achieved high-quality hybridization with collagen, improving mechanical properties and integrity, and decreased the enzyme-mediated degradation of the bonding interface by inhibiting collagenolytic activity. With the promising bonding durability of coapplied CBA, CRM may be the first dental adhesive to provide strong and long-lasting resin-dental collagen bonding without the additional conditioning step. The use of CBA results in high-quality hybrid layers that protect the resin-dentin interface from harmful biological and chemical activities commonly occurring in the oral environment.

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Ultrastructural and physicochemical characterization of pH receptive chlorhexidine‐loaded poly‐L‐glycolic acid‐modified orthodontic adhesive

Kamran

Alshahrani

Alnazeh

et al. 2021

Microscopy Res & Technique

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This study aims to evaluate the feasibility of chlorhexidine (CHX)-loaded poly-Lglycolic acid (PLGA) nanoparticles as a modifier of a commercial orthodontic adhesive via the assessment of physicochemical, biological, and mechanical properties at tooth-bracket interface. CHX-loaded PLGA nanoparticles were synthesized using double emulsion-solvent evaporation method and characterized using transmission electron microscopy and Raman analysis. CHX-loaded PLGA nanoparticles in Transbond XT orthodontic adhesive were prepared using two different concentrations of the CHX (25 and 50%) and characterized for degree of conversion (DC), antimicrobial, and cytotoxicity testing. Bonded specimens were tested for shear bond strength (SBS) and adhesive remnant index (ARI) at tooth-bracket interface. The synthesized PLGA nanoparticles averaged between 60 and 80 nm in size. After loading CHX inside PLGA nanoparticles, the morphology of the PLGA nanoparticles was considerably changed. Orthodontic bracket bonded with 25% CHX-loaded PLGA-modified adhesive demonstrated DC scores similar to control group. Both 25 and 50% CHXloaded PLGA-modified adhesive specimens showed higher antibacterial activity against S. mutans compared to control group. The least mean SBS values were exhibited by 50% CHX-loaded PLGA-modified adhesive samples, while a statistically significant difference was observed in the mean ARI values among all study groups at all-time points (p = .018). This study indicates that the addition of CHX-loaded PLGA nanoparticles in Transbond XT achieved stable bonds with enhanced antimicrobial and mechanical properties.

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Chlorhexidine-encapsulated mesoporous silica-modified dentin adhesive

Cited by 34 publications

References 44 publications

pH Dependent Delivery of Chlorhexidine From PLGA Grafted Mesoporous Silica Nanoparticles at Resin-Dentin Interface

pH Dependent Delivery of Chlorhexidine From PLGA Grafted Mesoporous Silica Nanoparticles at Resin-Dentin Interface

Evaluation of a Collagen-Reactive Monomer with Advanced Bonding Durability

Ultrastructural and physicochemical characterization of pH receptive chlorhexidine‐loaded poly‐L‐glycolic acid‐modified orthodontic adhesive

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